2024
Quantitative profiling of human translation initiation reveals elements that potently regulate endogenous and therapeutically modified mRNAs
Lewis C, Xie L, Bhandarkar S, Jin D, Abdallah K, Draycott A, Chen Y, Thoreen C, Gilbert W. Quantitative profiling of human translation initiation reveals elements that potently regulate endogenous and therapeutically modified mRNAs. Molecular Cell 2024, 85: 445-459.e5. PMID: 39706187, PMCID: PMC11780321, DOI: 10.1016/j.molcel.2024.11.030.Peer-Reviewed Original ResearchConceptsTranslation initiationUntranslated regionHuman 5'-untranslated regionChemically modified nucleotidesHigh-throughput methodRibosome recruitmentAlternative isoformsRegulatory elementsEnhanced translationDissecting mechanismsTherapeutic mRNANucleotideTherapeutic proteinsMRNADelivery of therapeutic proteinsSequenceMRNA vaccinesEndogenous RNAQuantitative profilingWidespread effectsTranslation outputRNATherapeuticsIsoformsCellular immunogenicityCellular translational enhancer elements that recruit eukaryotic initiation factor 3.
Koubek J, Kaur J, Bhandarkar S, Lewis C, Niederer R, Stanciu A, Aitken C, Gilbert W. Cellular translational enhancer elements that recruit eukaryotic initiation factor 3. RNA 2024, 31: 193-207. PMID: 39626887, PMCID: PMC11789482, DOI: 10.1261/rna.080310.124.Peer-Reviewed Original ResearchEukaryotic initiation factor 3Initiation factor 3Eukaryotic gene expressionTranslational enhancer elementMRNA-specific translationNative 5'-UTRFactor 3Initiation factor activityHigh-throughput approachRibosome recruitmentTranslation initiationRibosome densityBind mRNACellular mRNAsTranslational enhancerEnhancer elementsGrowing cellsGene expressionCentral playerFunctional relevanceRibosomeStress conditionsFactor activityAlternative pathwayMRNA
2019
Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation
Hosmillo M, Lu J, McAllaster MR, Eaglesham JB, Wang X, Emmott E, Domingues P, Chaudhry Y, Fitzmaurice TJ, Tung MK, Panas MD, McInerney G, Locker N, Wilen CB, Goodfellow IG. Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. ELife 2019, 8: e46681. PMID: 31403400, PMCID: PMC6739877, DOI: 10.7554/elife.46681.Peer-Reviewed Original ResearchConceptsViral positive-sense RNAFirst host factorHost factorsPositive-sense RNAPro-viral activityPositive-sense RNA virusesSense RNA virusesG3BP1 functionsRibosome recruitmentTranslation complexesTranslation initiationCRISPR screensProteomic analysisMurine norovirus infectionReplication complexSense RNANovel functionViral translationRNA virusesG3BP1Data uncoversNorovirus replicationLife cycleVPgGenus
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