2019
Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells.
Ventura A, Vassall A, Yurter A, Robinson E, Filler R, Hanlon D, Meeth K, Ezaldein H, Girardi M, Sobolev O, Bosenberg MW, Edelson RL. Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells. Journal Of Visualized Experiments 2019 PMID: 31157760, DOI: 10.3791/59370.Peer-Reviewed Original ResearchConceptsCutaneous T-cell lymphomaDendritic cellsCellular vaccinesClinical efficacyAnti-tumor T cell immunityVivo anti-tumor responsesMonocyte-derived dendritic cellsTumor cellsSyngeneic mouse tumor modelsImmunogenic dendritic cellsAnti-cancer immunityT cell immunityAnti-tumor responseHuman dendritic cellsT-cell lymphomaAnti-tumor effectsKey mechanistic driversApoptotic tumor cellsMouse tumor modelsCell immunitySafety profileCancer immunotherapyCell lymphomaMouse modelBlood samples
2016
Type 1 Interferon Regulates Inflammation Associated RBC Alloimmunization By Promoting Monocyte-Derived Dendritic Cell Erythrophagocytosis in Mice
Gibb D, Natarajan P, Liu J, Santhanakrishnan M, Iwasaki A, Hendrickson J. Type 1 Interferon Regulates Inflammation Associated RBC Alloimmunization By Promoting Monocyte-Derived Dendritic Cell Erythrophagocytosis in Mice. Blood 2016, 128: 19. DOI: 10.1182/blood.v128.22.19.19.Peer-Reviewed Original ResearchMonocyte-derived dendritic cellsReactivity of seraPeak antibody responseWT miceIfnar1-/- miceType 1 interferonDendritic cellsRBC transfusionPeripheral bloodAntibody responseRBC alloimmunizationAlloimmune responseSpecific IgGIgG antibodiesInflammatory diseasesMouse modelRBC antigensIndividual miceCCR2-/- miceFrequency of alloimmunizationHuman KEL glycoproteinFlow cytometric crossmatchActivation marker expressionDendritic cell activationRole of inflammationSingle cell variability in pro-inflammatory and antiviral gene responses in dendritic cells
Fribourg Casajuana M, Hartmann B, Tabbaa O, Ramos I, Zaslavsky E, Nudelman G, Albrecht R, Merad M, Hayot F, Jayaprakash C, Kleinstein S, Garcia-Sastre A, Sealfon S. Single cell variability in pro-inflammatory and antiviral gene responses in dendritic cells. The Journal Of Immunology 2016, 196: 202.29-202.29. DOI: 10.4049/jimmunol.196.supp.202.29.Peer-Reviewed Original ResearchDendritic cellsI IFNsI interferonImmune responsePeripheral Blood Plasmacytoid Dendritic CellsBlood plasmacytoid dendritic cellsMonocyte-derived dendritic cellsType I IFNsDendritic cell populationsPlasmacytoid dendritic cellsAppropriate immune responseHuman peripheral bloodType I interferonAntiviral gene responsesRIG-I inductionH1N1 virusAutoimmune diseasesPeripheral bloodChronic infectionNoxious stimuliViral infectionImmune signalsAntiviral ISGsCell responsesISG group
2015
Lymphocyte phosphatase‐associated phosphoprotein proteoforms analyzed using monoclonal antibodies
Filatov A, Kruglova N, Meshkova T, Mazurov D. Lymphocyte phosphatase‐associated phosphoprotein proteoforms analyzed using monoclonal antibodies. Clinical & Translational Immunology 2015, 4: e44. PMID: 26682052, PMCID: PMC4673442, DOI: 10.1038/cti.2015.22.Peer-Reviewed Original ResearchLymphocyte phosphatase-associated phosphoproteinActivation of lymphocytesMonocyte-derived dendritic cellsPan-lymphocyte markerLPAP phosphorylationExpression of CD45Levels of receptorFunction of LPAPNK cellsDendritic cellsRed blood cellsB cellsTwo-dimensional protein gel electrophoresisLck kinaseSignaling molecule phosphorylationCD45LymphocytesCD45 moleculesMonoclonal antibodiesSites of phosphorylationBlood cellsProtein gel electrophoresisActivity of CD45Cell typesPhosphatase CD45
2014
Blood monocytes expressing CCR6 and monocyte-derived dendritic cells in dermis and epidermis are critical for IL-23-induced psoriasis-like inflammation in mice (CAM5P.238)
Singh T, Zhang H, Farber J. Blood monocytes expressing CCR6 and monocyte-derived dendritic cells in dermis and epidermis are critical for IL-23-induced psoriasis-like inflammation in mice (CAM5P.238). The Journal Of Immunology 2014, 192: 180.9-180.9. DOI: 10.4049/jimmunol.192.supp.180.9.Peer-Reviewed Original ResearchCCR6-/- miceIL-23 injectionDendritic cellsPsoriasis-like inflammationIL-23Blood monocytesAccumulation of dendritic cellsMonocyte-derived dendritic cellsHuman studiesLangerin-DTR micePsoriasis-like pathologyExpression of CCR6Monocyte-derived cellsLangerin+ cellsInflamed skinMyeloid cellsNon-monocyticIL-22Injected i.Psoriatic skinCCR6InflammationMiceMouse skinMonocytesPattern Recognition Receptor Signaling in Human Dendritic Cells is Enhanced by ICOS Ligand and Modulated by the Crohn’s Disease ICOSLG Risk Allele
Hedl M, Lahiri A, Ning K, Cho JH, Abraham C. Pattern Recognition Receptor Signaling in Human Dendritic Cells is Enhanced by ICOS Ligand and Modulated by the Crohn’s Disease ICOSLG Risk Allele. Immunity 2014, 40: 734-746. PMID: 24837102, PMCID: PMC4157904, DOI: 10.1016/j.immuni.2014.04.011.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedCrohn DiseaseDendritic CellsEnzyme ActivationGTP-Binding ProteinsHL-60 CellsHumansInducible T-Cell Co-Stimulator LigandInducible T-Cell Co-Stimulator ProteinJNK Mitogen-Activated Protein KinasesMacrophagesNeoplasm ProteinsNF-kappa BNod2 Signaling Adaptor ProteinPhosphorylationPolymorphism, Single NucleotideProtein Kinase CReceptors for Activated C KinaseReceptors, Cell SurfaceReceptors, Pattern RecognitionRNA InterferenceRNA, Small InterferingSignal TransductionConceptsMonocyte-derived dendritic cellsInflammatory bowel diseaseCytokine secretionDendritic cellsImmune homeostasisICOS ligandHuman monocyte-derived dendritic cellsPattern recognition receptor signalingRisk allelesIntestinal immune homeostasisCrohn's disease phenotypeHuman dendritic cellsCostimulatory molecule ICOSOligomerization domain 2NF-κB activationDisease phenotypePattern recognition receptorsICOSL expressionBowel diseaseReceptor signalingRisk carriersSecretionHomeostasisKinases PKCSignaling
2013
Mammaglobin B (SCGB2A1) is a novel tumour antigen highly differentially expressed in all major histological types of ovarian cancer: implications for ovarian cancer immunotherapy
Bellone S, Tassi R, Betti M, English D, Cocco E, Gasparrini S, Bortolomai I, Black JD, Todeschini P, Romani C, Ravaggi A, Bignotti E, Bandiera E, Zanotti L, Pecorelli S, Ardighieri L, Falchetti M, Donzelli C, Siegel ER, Azodi M, Silasi DA, Ratner E, Schwartz PE, Rutherford TJ, Santin AD. Mammaglobin B (SCGB2A1) is a novel tumour antigen highly differentially expressed in all major histological types of ovarian cancer: implications for ovarian cancer immunotherapy. British Journal Of Cancer 2013, 109: 462-471. PMID: 23807163, PMCID: PMC3721400, DOI: 10.1038/bjc.2013.315.Peer-Reviewed Original ResearchConceptsCytotoxic T lymphocytesMajor histological typesOvarian cancerDendritic cellsHistological typeMammaglobin BType 1 cytokine profileMonocyte-derived dendritic cellsClass I monoclonal antibodiesTumor cellsMetastatic/recurrentOvarian cancer immunotherapyAutologous tumor cellsImmunotherapy of patientsIntracellular cytokine expressionNovel tumor antigensOvarian cancer typesTumor rejection antigensRecurrent diseaseCytokine profileCytokine expressionOvarian tumorsCancer immunotherapyCTL populationsOvarian carcinoma
2012
Clinical regressions and broad immune activation following combination therapy targeting human NKT cells in myeloma
Richter J, Neparidze N, Zhang L, Nair S, Monesmith T, Sundaram R, Miesowicz F, Dhodapkar KM, Dhodapkar MV. Clinical regressions and broad immune activation following combination therapy targeting human NKT cells in myeloma. Blood 2012, 121: 423-430. PMID: 23100308, PMCID: PMC3548165, DOI: 10.1182/blood-2012-06-435503.Peer-Reviewed Original ResearchConceptsHuman iNKT cellsINKT cellsCombination therapyAntitumor T-cell immunitySerum soluble IL2 receptorMonocyte-derived dendritic cellsNatural killer T cellsBroad immune activationLow-dose lenalidomideSoluble IL2 receptorCycles of therapyHuman NKT cellsT cell immunityKiller T cellsInnate immune cellsInduction of eosinophiliaActivation of monocytesPrevention of cancerCycles of combinationAsymptomatic myelomaClinical myelomaMeasurable diseaseClinical regressionClinical responseNKT cells
2009
Hepatitis C virus modulates human monocyte‐derived dendritic cells
Eksioglu E, Bess J, Zhu H, Xu Y, Dong H, Elyar J, Nelson D, Liu C. Hepatitis C virus modulates human monocyte‐derived dendritic cells. Journal Of Viral Hepatitis 2009, 17: 757-769. PMID: 20051006, PMCID: PMC3731759, DOI: 10.1111/j.1365-2893.2009.01231.x.Peer-Reviewed Original ResearchConceptsHepatitis C virusNewcastle disease virusHCV infectionC virusHuman monocyte-derived dendritic cellsViral titresMonocyte-derived dendritic cellsType 1 IFN responseFlow cytometryType 1 interferon responseMaturation of DCsDendritic cell responsesReal-time reverse transcriptase-polymerase chain reactionReverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionHuh-7.5 cellsDC numbersDendritic cellsImmature DCsIFNβ expressionJFH-1Control monocytesHost immunityViral evasionIFN response
2008
Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naïve Donor Immune Response Towards a Type‐1 Cytokine Profile
Reis E, Carmo T, Athanazio R, Reis M, Harn D. Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naïve Donor Immune Response Towards a Type‐1 Cytokine Profile. Scandinavian Journal Of Immunology 2008, 68: 169-176. PMID: 18565118, DOI: 10.1111/j.1365-3083.2008.02131.x.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsSchistosoma mansoni soluble egg antigenDendritic cellsCytokine productionAutologous peripheral blood mononuclear cellsType 1 cytokine profileMonocyte-derived dendritic cellsDonor dendritic cellsDonor immune responsesTh1-type responseT cell subsetsBlood mononuclear cellsIFN-gamma productionT cell epitopesMultiple antigen peptideMajor cell populationRecall stimulationCytokine profileIL-10Egg antigenMononuclear cellsT cellsImmune responseIFN-gammaMouse model
2005
HPV16/18 E7-pulsed dendritic cell vaccination in cervical cancer patients with recurrent disease refractory to standard treatment modalities
Santin AD, Bellone S, Palmieri M, Ravaggi A, Romani C, Tassi R, Roman JJ, Burnett A, Pecorelli S, Cannon MJ. HPV16/18 E7-pulsed dendritic cell vaccination in cervical cancer patients with recurrent disease refractory to standard treatment modalities. Gynecologic Oncology 2005, 100: 469-478. PMID: 16249018, DOI: 10.1016/j.ygyno.2005.09.040.Peer-Reviewed Original ResearchConceptsCervical cancer patientsStandard treatment modalityAutologous dendritic cellsT cell responsesDendritic cellsCancer patientsTreatment modalitiesHPV16/18 E7Clinical responseIFN-gammaAutologous monocyte-derived dendritic cellsE7 oncoproteinsLate-stage cervical cancer patientsCell responsesMonocyte-derived dendritic cellsHuman recombinant interleukin-2Active vaccination strategiesHPV18 E7 oncoproteinLimited tumor burdenTreatment-induced immunosuppressionAutologous tumor cellsDendritic cell vaccinationObjective clinical responsesEarly-stage diseaseType hypersensitivity reaction
2000
Macaque blood‐derived antigen‐presenting cells elicit SIV‐specific immune responses
Zhu Y, Koo K, Bradshaw J, Sutton W, Kuller L, Bucala R, Anderson D, Mossman S, Villinger F, Haigwood N. Macaque blood‐derived antigen‐presenting cells elicit SIV‐specific immune responses. Journal Of Medical Primatology 2000, 29: 182-192. PMID: 11085581, DOI: 10.1034/j.1600-0684.2000.290312.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationAntigen-Presenting CellsAntigens, CDAntigens, CD34Antigens, ViralCells, CulturedDendritic CellsGenes, ReporterGreen Fluorescent ProteinsHumansImmunity, CellularImmunoglobulinsLuminescent ProteinsMacaca fascicularisMembrane GlycoproteinsMonocytesSAIDS VaccinesSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusTransfectionTransplantation, AutologousConceptsMonocyte-derived dendritic cellsDendritic cellsAntigen-presenting cellsInterleukin-4Autologous monocyte-derived dendritic cellsSIV-specific immune responsesAutologous dendritic cellsDC marker CD83Macaque dendritic cellsGranulocyte-macrophage colonyMurine fibrocytesSIV vaccinesSIV antigensHumoral responseAntigen presentationImmune responseCharacteristic cellular morphologyDNA expression vectorsFibrocytesMacaca fascicularisSIVProtein expression plasmidsMacaquesExpression plasmidCells
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply