2020
Identification of a novel splice variant for mouse and human interleukin-5
Shilovskiy I, Andreev S, Mazurov D, Barvinskaia E, Bolotova S, Nikolskii A, Sergeev I, Maerle A, Kudlay D, Khaitov M. Identification of a novel splice variant for mouse and human interleukin-5. Heliyon 2020, 6: e03586. PMID: 32211550, PMCID: PMC7082524, DOI: 10.1016/j.heliyon.2020.e03586.Peer-Reviewed Original ResearchSplice variantsMIL-5Exon 2Human 293T cell linePairs of primersAnalysis of PCR productsSplice-specific primersMRNA splicing isoformsAlternative splice variantsMouse IL-5IL-5Fluorescence microscopy examinationRT-PCRAlternative isoformsHuman interleukin-5Human IL-5Coding sequenceAlternative splicingSequence analysisSpliced isoformsSplice formsEndoplasmic reticulumPCR productsPeripheral blood of healthy personsExpression plasmid
2008
A novel missense mutation in SLC34A3 that causes hereditary hypophosphatemic rickets with hypercalciuria in humans identifies threonine 137 as an important determinant of sodium-phosphate cotransport in NaPi-IIc
Jaureguiberry G, Carpenter TO, Forman S, Jüppner H, Bergwitz C. A novel missense mutation in SLC34A3 that causes hereditary hypophosphatemic rickets with hypercalciuria in humans identifies threonine 137 as an important determinant of sodium-phosphate cotransport in NaPi-IIc. American Journal Of Physiology. Renal Physiology 2008, 295: f371-f379. PMID: 18480181, PMCID: PMC2519180, DOI: 10.1152/ajprenal.00090.2008.Peer-Reviewed Original ResearchMeSH KeywordsAdultAllelesAnimalsBase SequenceExocytosisFamilial Hypophosphatemic RicketsFemaleHaplotypesHumansHypercalciuriaKidneyMaleMolecular Sequence DataMutation, MissenseOocytesOpossumsPhosphatesPolymorphism, Single NucleotideSodiumSodium-Phosphate Cotransporter ProteinsSodium-Phosphate Cotransporter Proteins, Type IIcThreonineXenopus laevisConceptsEncoding enhanced green fluorescent proteinHereditary hypophosphatemic ricketsNaPi-IIcSodium-phosphate cotransporterLoss of expressionAmino acid residuesSodium-phosphate cotransportGreen fluorescence proteinImportant functional roleComplete lossOpossum kidneyHypophosphatemic ricketsXenopus laevis oocytesNovel missense mutationPaternal alleleWild-typeFunctional analysisFluorescence proteinNH2 terminusAcid residuesApical patchesCompound heterozygous mutationsExpression plasmidFunctional roleRecurrent kidney stones
2000
Introduction of the human growth hormone gene into the guinea pig mammary gland by in vivo transfection promotes sustained expression of human growth hormone in the milk throughout lactation
Hens J, Amstutz M, Schanbacher F, Mather I. Introduction of the human growth hormone gene into the guinea pig mammary gland by in vivo transfection promotes sustained expression of human growth hormone in the milk throughout lactation. Biochimica Et Biophysica Acta 2000, 1523: 161-171. PMID: 11042380, DOI: 10.1016/s0304-4165(00)00117-3.Peer-Reviewed Original ResearchConceptsHuman growth hormone geneGrowth hormone geneGuinea-pig mammary glandPig mammary glandHormone geneBovine mammary cellsTransfection of cellsMammary glandGene promoterBiological roleRecombinant proteinsTransgenic animalsExpression plasmidMammary cellsHuman growth hormoneCytomegalovirus promoterTransfectionSustained expressionGenesPromoterGrowth hormonePlasmid DNAMammary tissueExpressionTransfection complexesMacaque blood‐derived antigen‐presenting cells elicit SIV‐specific immune responses
Zhu Y, Koo K, Bradshaw J, Sutton W, Kuller L, Bucala R, Anderson D, Mossman S, Villinger F, Haigwood N. Macaque blood‐derived antigen‐presenting cells elicit SIV‐specific immune responses. Journal Of Medical Primatology 2000, 29: 182-192. PMID: 11085581, DOI: 10.1034/j.1600-0684.2000.290312.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationAntigen-Presenting CellsAntigens, CDAntigens, CD34Antigens, ViralCells, CulturedDendritic CellsGenes, ReporterGreen Fluorescent ProteinsHumansImmunity, CellularImmunoglobulinsLuminescent ProteinsMacaca fascicularisMembrane GlycoproteinsMonocytesSAIDS VaccinesSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusTransfectionTransplantation, AutologousConceptsMonocyte-derived dendritic cellsDendritic cellsAntigen-presenting cellsInterleukin-4Autologous monocyte-derived dendritic cellsSIV-specific immune responsesAutologous dendritic cellsDC marker CD83Macaque dendritic cellsGranulocyte-macrophage colonyMurine fibrocytesSIV vaccinesSIV antigensHumoral responseAntigen presentationImmune responseCharacteristic cellular morphologyDNA expression vectorsFibrocytesMacaca fascicularisSIVProtein expression plasmidsMacaquesExpression plasmidCells
1999
TNF recruits TRADD to the plasma membrane but not the trans-Golgi network, the principal subcellular location of TNF-R1.
Jones S, Ledgerwood E, Prins J, Galbraith J, Johnson D, Pober J, Bradley J. TNF recruits TRADD to the plasma membrane but not the trans-Golgi network, the principal subcellular location of TNF-R1. The Journal Of Immunology 1999, 162: 1042-8. PMID: 9916731, DOI: 10.4049/jimmunol.162.2.1042.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDAortaBrefeldin ACattleCell CompartmentationCell Line, TransformedCell MembraneEndothelium, VascularGolgi ApparatusHumansMicroscopy, ConfocalProteinsReceptors, Tumor Necrosis FactorReceptors, Tumor Necrosis Factor, Type ISubcellular FractionsTNF Receptor-Associated Factor 1TransfectionTumor Necrosis Factor-alphaU937 CellsConceptsTrans-Golgi networkPlasma membraneTNF-R1Golgi regionConfocal immunofluorescence microscopyHuman endothelial cell line ECV304Endothelial cell line ECV304Receptor-mediated endocytosisAdaptor proteinSubcellular localizationSubcellular locationCell fractionationBovine aortic endothelial cellsCoimmunoprecipitation studiesEndothelial cellsTRADDCell line U937Golgi apparatusSubcellular interactionsWestern blot analysisCell extractsMonocyte cell line U937Expression plasmidGolgiImmunofluorescence microscopy
1996
Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma.
Kuang Y, Wu Y, Smrcka A, Jiang H, Wu D. Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 2964-2968. PMID: 8610151, PMCID: PMC39743, DOI: 10.1073/pnas.93.7.2964.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDBlotting, WesternCell LineChlorocebus aethiopsDNA, ComplementaryGlutamic AcidGTP-Binding ProteinsHumansIsoenzymesKidneyMacromolecular SubstancesMammalsMutagenesis, Site-DirectedPoint MutationPolymerase Chain ReactionReceptor, Anaphylatoxin C5aReceptors, ComplementRecombinant Fusion ProteinsRecombinant ProteinsTransfectionType C PhospholipasesConceptsCOS-7 cellsGlutathione S-transferaseGbeta gammaPLC-beta2Fusion proteinBeta gamma subunitsLigand-induced activationAlpha1B-adrenergic receptorMammalian expression plasmidX boxG proteinsY boxExpression plasmidAmino acidsS-transferaseProteinSequenceResiduesCellsActivationBeta2GalphaGbetaC5a receptorCDNA
1995
The hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease.
Jwang B, Dewing P, Fikrig E, Flavell RA. The hook protein of Borrelia burgdorferi, encoded by the flgE gene, is serologically recognized in Lyme disease. MSphere 1995, 2: 609-15. PMID: 8548542, PMCID: PMC170207, DOI: 10.1128/cdli.2.5.609-615.1995.Peer-Reviewed Original ResearchConceptsFlgE proteinFlgE geneEscherichia coli expression plasmidPrimary protein sequenceB. burgdorferi N40Hook geneWhole cell lysatesHook proteinLinear chromosomesPeriplasmic flagellaProtein sequencesFlgERecombinant proteinsB. burgdorferiBasal bodiesFlagellar filamentsWestern blot analysisBorrelia burgdorferiGenesExpression plasmidProteinBlot analysisNegative bacteriaMurine antiseraLyme disease
1994
Restoration of aryl hydrocarbon (Ah) responsiveness in MDA-MB-231 human breast cancer cells by transient expression of the estrogen receptor
Thomsen J, Wang X, Hines R, Safe S. Restoration of aryl hydrocarbon (Ah) responsiveness in MDA-MB-231 human breast cancer cells by transient expression of the estrogen receptor. Carcinogenesis 1994, 15: 933-937. PMID: 8200098, DOI: 10.1093/carcin/15.5.933.Peer-Reviewed Original ResearchConceptsMDA-MB-231 cellsHuman breast cancer cellsMDA-MB-231 human breast cancer cellsAh-responsivenessBreast cancer cellsNM TCDDHuman estrogen receptor expression plasmidAryl hydrocarbon responsivenessCancer cellsReceptor expression plasmidAryl hydrocarbon receptorProgesterone receptorEstrogen receptorResponsive plasmidCYP1A1 gene expressionHydrocarbon receptorReceptorsCell linesCYP1A1 geneCAT activityExpression of nuclearParallel studyHuman CYP1A1 geneCellsExpression plasmid
1986
Sorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells.
Gottlieb T, Gonzalez A, Rizzolo L, Rindler M, Adesnik M, Sabatini D. Sorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells. Journal Of Cell Biology 1986, 102: 1242-1255. PMID: 3007530, PMCID: PMC2114186, DOI: 10.1083/jcb.102.4.1242.Peer-Reviewed Original ResearchConceptsG proteinsCell surfaceVesicular stomatitis virusApical plasma membrane domainPlasma membrane domainsPlasma membrane proteinsCell surface domainsApical cell surfaceMadin-Darby canine kidney cellsG protein moleculesViral glycoproteinsLysosome-like vesiclesCanine kidney cellsProtein synthesis inhibitorMembrane domainsMembrane proteinsSurface domainsBasolateral domainEukaryotic expression plasmidTransfection experimentsExpression plasmidProteinPresence of chloroquineApical surfaceProtein molecules
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