2025
Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2
Hasan S, Ghani N, Zhao X, Good J, Liu C. Exogenous pyruvate is therapeutic against colitis by targeting cytosolic phospholipase A2. Genes & Diseases 2025, 12: 101571. DOI: 10.1016/j.gendis.2025.101571.Peer-Reviewed Original ResearchInflammatory bowel diseaseUlcerative colitisBowel diseaseDextran sodium sulfate-induced colitisChronic inflammatory bowel diseaseUlcerative colitis pathogenesisTight junction proteinsPhase of inflammationCytosolic phospholipase A2Immune dysregulationColitis pathogenesisReal-time PCRNFkB translocationCellular metabolitesColon histologyJunction proteinsInflammatory diseasesColitis modelQuantitative real-time PCRTherapeutic effectUlcerative colitis modelAction of pyruvateImmunofluorescence stainingColitisRNA sequencing analysis
2024
Efficient and selective kidney targeting by chemically modified carbohydrate conjugates
Kumar V, Wahane A, Tham M, Somlo S, Gupta A, Bahal R. Efficient and selective kidney targeting by chemically modified carbohydrate conjugates. Molecular Therapy 2024, 32: 4383-4400. PMID: 39532098, PMCID: PMC11638880, DOI: 10.1016/j.ymthe.2024.10.020.Peer-Reviewed Original ResearchProximal convoluted tubulesPeptide nucleic acidConvoluted tubulesNucleic acid analogsDecreased collagen depositionAntisense peptide nucleic acidFibrosis mouse modelCarbohydrate conjugatesKidney disease modelsSystemic deliveryFibrosis progressionSmall moleculesKidney cell lineAdverse reactionsMiR-33Mouse modelBiodistribution studiesImmunofluorescence stainingCollagen depositionKidney targetingTargeted deliveryLigandCell linesEndocytic uptakeAcid analogsLiver Sinusoidal Endothelial Cells Contribute to Portal Hypertension Through Collagen Type IV–Driven Sinusoidal Remodeling
Gan C, Yaqoob U, Lu J, Xie M, Anwar A, Jalan-Sakrikar N, Jerez S, Sehrawat T, Navarro-Corcuera A, Kostallari E, Habash N, Cao S, Shah V. Liver Sinusoidal Endothelial Cells Contribute to Portal Hypertension Through Collagen Type IV–Driven Sinusoidal Remodeling. JCI Insight 2024, 9: e174775. PMID: 38713515, PMCID: PMC11382879, DOI: 10.1172/jci.insight.174775.Peer-Reviewed Original ResearchLiver sinusoidal endothelial cellsPortal hypertensionSinusoidal remodelingSinusoidal endothelial cellsSinusoidal resistanceComplication of liver cirrhosisEndothelial cellsSinusoidal endothelial cells in vitroEnhancer-promoter interactionsEpigenome editing approachesEndothelial cells in vitroChronic liver injuryCells in vitroMouse fibrotic liversCollagen type IVLiver cirrhosisGene mutationsExpression regulationLiver fibrosisLiver injuryEpigenetic repressionLiver diseaseCellular sourceCol4 expressionImmunofluorescence staining
2021
Shotgun Immunoproteomics for Identification of Nonhuman Leukocyte Antigens Associated With Cellular Dysfunction in Heart Transplant Rejection
Gates K, Panicker A, Biendarra-Tiegs S, Vetr N, Higuita M, Nelson T, Pereira N, Griffiths L. Shotgun Immunoproteomics for Identification of Nonhuman Leukocyte Antigens Associated With Cellular Dysfunction in Heart Transplant Rejection. Transplantation 2021, 106: 1376-1389. PMID: 34923540, DOI: 10.1097/tp.0000000000004012.Peer-Reviewed Original ResearchConceptsAntibody-mediated rejectionHeart transplant recipientsAntigen-specific responsesNon-HLAPrevalence antigensAcute rejectionTransplant recipientsAbsence of donor-specific anti-HLA antibodiesDonor-specific anti-HLA antibodiesNon-HLA antibody responsesAntibody-mediated rejection patientsNon-AMR patientsAnti-HLA antibodiesHeart transplant rejectionPatient immunoglobulin GHigh-prevalence antigenEndothelial cell functionEndomyocardial biopsyAllograft rejectionGraft rejectionHeart biopsiesTransplant rejectionAntibody responseImmunofluorescence stainingPatientsCD209L/L-SIGN and CD209/DC-SIGN Act as Receptors for SARS-CoV‑2
Amraei R, Yin W, Napoleon M, Suder E, Berrigan J, Zhao Q, Olejnik J, Chandler K, Xia C, Feldman J, Hauser B, Caradonna T, Schmidt A, Gummuluru S, Mühlberger E, Chitalia V, Costello C, Rahimi N. CD209L/L-SIGN and CD209/DC-SIGN Act as Receptors for SARS-CoV‑2. ACS Central Science 2021, 7: 1156-1165. PMID: 34341769, PMCID: PMC8265543, DOI: 10.1021/acscentsci.0c01537.Peer-Reviewed Original ResearchSARS-CoV-2 spike receptor-binding domainSARS-CoV-2SARS-CoV-2 entryL-SIGNSARS-CoV-2 infectionCD209/DC-SIGNSpike receptor-binding domainAntiviral drug developmentReceptor-binding domainCell typesHuman endothelial cellsDC-SIGNAlternative receptorLow expressionEndothelial cellsImmunofluorescence stainingCD209Virus entryACE2Disease-relevant cell typesProminent expressionCD209LKidney epitheliumReceptorsDrug development
2020
Identification of Podocyte Cargo Proteins by Proteomic Analysis of Clathrin-Coated Vesicles.
Groener M, Wang Y, Cross E, Tian X, Ebenezer K, Baik E, Pedigo C, Schiffer M, Inoue K, Ishibe S. Identification of Podocyte Cargo Proteins by Proteomic Analysis of Clathrin-Coated Vesicles. Kidney360 2020, 1: 480-490. PMID: 35368594, PMCID: PMC8809311, DOI: 10.34067/kid.0000212020.Peer-Reviewed Original ResearchConceptsClathrin-coated vesiclesCargo proteinsProteomic analysisPodocyte healthPrimary podocyte cultureTransferrin receptor protein 1Endocytic traffickingSignificant proteinsLiquid chromatography-mass spectrometryPodocyte culturesHigh abundanceGenetic ablationSmall vesiclesCCV fractionsProteinProtein 1Protein markersClathrinVesiclesMultiple protein markersDiphtheria toxinFundamental roleMouse kidneyImmunofluorescence stainingCargo
2016
Differential expression pattern of Twist1 in mouse preimplantation embryos suggests its multiple roles during early development
Sozen B, Pehlivanoglu S, Demir N. Differential expression pattern of Twist1 in mouse preimplantation embryos suggests its multiple roles during early development. Journal Of Assisted Reproduction And Genetics 2016, 33: 1533-1540. PMID: 27544279, PMCID: PMC5125151, DOI: 10.1007/s10815-016-0794-1.Peer-Reviewed Original ResearchConceptsMouse preimplantation embryosInner cell massDouble immunofluorescence stainingCulture of embryosEarly embryo developmentExpression of Twist1Blastocyst developmentPreimplantation developmentPreimplantation embryosPresence of high glucoseEarly stages of embryosCells of embryosBlastocystImmunofluorescence stainingTwist-related protein 1Stages of embryosRegulation of TwistPurposeThe purposeUniform stainingLineage segregationTwist1 expressionProtein 1Protein levelsEmbryo developmentTwist1
2014
β-catenin and its alteration in an experimental model of diabetic nephropathy.
Park S, Ahn E, Ha T, Shin J. β-catenin and its alteration in an experimental model of diabetic nephropathy. Iranian Journal Of Kidney Diseases 2014, 8: 299-309. PMID: 25001136.Peer-Reviewed Original ResearchConceptsAdvanced glycation endproductsGlomerular epithelial cellsBeta-CateninPresence of advanced glycation endproductsExperimental models of diabetic nephropathyDevelopment of kidney dysfunctionEpithelial cellsRat glomerular epithelial cellsModel of diabetic nephropathyRedistribution of beta-cateninBeta-catenin stainingBeta-catenin proteinBeta-catenin mRNAReverse transcription-polymerase chain reactionCadherin protein complexPodocytes in vitroTranscription-polymerase chain reactionNormal glucose conditionsWestern blot analysisNo significant changesKidney dysfunctionBeta-catenin productionDiabetic nephropathyB-cateninImmunofluorescence staining
2013
Infections by Leptospira interrogans, Seoul Virus, and Bartonella spp. Among Norway Rats (Rattus norvegicus) from the Urban Slum Environment in Brazil
Costa F, Porter FH, Rodrigues G, Farias H, de Faria MT, Wunder EA, Osikowicz LM, Kosoy MY, Reis MG, Ko AI, Childs JE. Infections by Leptospira interrogans, Seoul Virus, and Bartonella spp. Among Norway Rats (Rattus norvegicus) from the Urban Slum Environment in Brazil. Vector-Borne And Zoonotic Diseases 2013, 14: 33-40. PMID: 24359425, PMCID: PMC3880909, DOI: 10.1089/vbz.2013.1378.Peer-Reviewed Original ResearchConceptsSeoul virusEnzyme-linked immunosorbent assayLeptospiral carriageBartonella sppLeptospira sppZoonotic pathogensLeptospira interrogansNorway ratsReservoir hostsSignificant morbidityHigh prevalenceCommon pathogensRatsWestern blottingImmunofluorescence stainingImmunosorbent assayPrevalenceInfectionPrevalence informationTransmission dynamicsSpecies of BartonellaMultiple pathogensNorway rat populationsUrban slum environmentsCarriageIsolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology
Wu Y, Deighan C, Miller B, Balasubramanian P, Lustberg M, Zborowski M, Chalmers J. Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology. Methods 2013, 64: 169-182. PMID: 24056212, PMCID: PMC3874448, DOI: 10.1016/j.ymeth.2013.09.006.Peer-Reviewed Original ResearchConceptsCancer patientsMetastatic breast cancer patientsBreast cancer patientsPeripheral blood samplesRed blood cell lysisRare cellsNon-hematopoietic cellsBlood cell lysisImmunomagnetic depletionCD45 antibodyBlood samplesLog depletionPatientsImmunofluorescence stainingNucleated cellsWhole bloodBloodCD45RBC lysisCellsCell lysisLysisAntibodies
2005
HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane
Mazurov D, Heidecker G, Derse D. HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane. Virology 2005, 346: 194-204. PMID: 16325219, DOI: 10.1016/j.virol.2005.10.033.Peer-Reviewed Original ResearchConceptsHTLV-1 GagHTLV-1T cellsPresence of bacterial superantigensPlasma membraneGag proteinHIV-1 GagMembrane of T cellsAssociation of GagViral envelope glycoproteinsPlasma membrane of T cellsRaji B cellsCell extractsBacterial superantigensAntibody-mediated cross-linkingB cellsHIV-1Confocal image analysisEnvelope glycoproteinTetraspanin-enriched microdomainsJurkat T cellsImmunofluorescence stainingImmune synapseGag multimerizationIntercellular adhesion
1997
Identification and characterization of homologues of the Exocyst component Sec10p
Guo W, Roth D, Gatti E, De Camilli P, Novick P. Identification and characterization of homologues of the Exocyst component Sec10p. FEBS Letters 1997, 404: 135-139. PMID: 9119050, DOI: 10.1016/s0014-5793(97)00109-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBrainCloning, MolecularCOS CellsExocytosisFungal ProteinsGolgi ApparatusHumansMammalsMolecular Sequence DataPolymerase Chain ReactionRecombinant ProteinsRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidTranscription, GeneticTransfectionVesicular Transport ProteinsConceptsC. elegans proteinsCharacterization of homologuesAmino acid identityBroad tissue distributionGolgi trafficMammalian counterpartsYeast SaccharomycesAcid identityGene productsCOS cellsWestern blot analysisSec10pPeripheral cytoplasmExocystBlot analysisProteinTissue distributionImmunofluorescence stainingSec8pCellsSaccharomycesCloningHomologuesExocytosisCytoplasm
1982
Localization of Collagen Type IV in Basement Membranes by Monoclonal Antibodies
FOELLMER H, MADRI J, WYATT R, FURTHMAYR H. Localization of Collagen Type IV in Basement Membranes by Monoclonal Antibodies. Protides Of The Biological Fluids 1982, 29: 773-776. DOI: 10.1016/b978-0-08-027988-6.50180-4.Peer-Reviewed Original ResearchMonoclonal antibodiesType IV collagenBasement membraneType IVFrozen sectionsNormal human kidneyIV collagenSheep anti-rabbit IgGRabbit anti-mouse IgGBowman's capsuleCollagen type IVThick frozen sectionsHuman kidneyHuman placentaImmunofluorescence stainingAntibodiesKidneySpecific stainingSimilar labeling patternIgGMouse IgGAnti-rabbit IgGMouse EHS tumorStainingCollagen
1980
Ultrastructural localization of fibronectin and laminin in the basement membranes of the murine kidney.
Madri J, Roll F, Furthmayr H, Foidart J. Ultrastructural localization of fibronectin and laminin in the basement membranes of the murine kidney. Journal Of Cell Biology 1980, 86: 682-687. PMID: 6995470, PMCID: PMC2111490, DOI: 10.1083/jcb.86.2.682.Peer-Reviewed Original ResearchConceptsTubular basement membraneMesangial areaBowman's capsuleMesangial matrixBasement membraneFrozen sectionsNormal murine kidneyMurine kidneyAffinity-purified rabbit antibodiesConventional frozen sectionsGlomerular basement membraneSheep anti-rabbit IgGLaminin labellingThick frozen sectionsImmunofluorescence stainingSpecific antibodiesRabbit antibodiesLamina rara internaMesangial cell processesKidneyUltrastructural levelCapsuleAntibodiesAnti-rabbit IgGLaminin
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