2011
A Viral Nuclear Noncoding RNA Binds Re-localized Poly(A) Binding Protein and Is Required for Late KSHV Gene Expression
Borah S, Darricarrère N, Darnell A, Myoung J, Steitz JA. A Viral Nuclear Noncoding RNA Binds Re-localized Poly(A) Binding Protein and Is Required for Late KSHV Gene Expression. PLOS Pathogens 2011, 7: e1002300. PMID: 22022268, PMCID: PMC3192849, DOI: 10.1371/journal.ppat.1002300.Peer-Reviewed Original ResearchConceptsPAN RNAKaposi's sarcoma-associated herpesvirusNuclear noncoding RNANuclear noncoding RNAsShutoff effectLytic phaseKSHV gene expressionRepertoire of functionsTail of mRNATransient transfection experimentsConsequence of expressionLate viral proteinsNoncoding RNAsExonuclease proteinNuclear RNAProtein C1Translation efficiencyHost mRNAsMRNA stabilityGene expressionUnknown functionTransfection experimentsViral mRNAsPABPC1Binding protein
2005
Multiple isoforms of the KC1 cotransporter are expressed in sickle and normal erythroid cells
Crable SC, Hammond SM, Papes R, Rettig RK, Zhou GP, Gallagher PG, Joiner CH, Anderson KP. Multiple isoforms of the KC1 cotransporter are expressed in sickle and normal erythroid cells. Experimental Hematology 2005, 33: 624-631. PMID: 15911086, DOI: 10.1016/j.exphem.2005.02.006.Peer-Reviewed Original ResearchConceptsErythroid cellsKCC isoformsMultiple isoformsSplicing variantsStructure/function studiesKCl cotransporterWild-type cellsHuman erythroid cellsDifferentiated erythroid precursorsCl-dependent K fluxTransient transfection experimentsNormal erythroid cellsIsoform expression patternCotransporter activityRed cellsKCC genesExpression patternsReticulocyte maturationHuman red cellsKCC1 geneTransfection experimentsReverse transcriptase-PCRSplice variantsRelative abundanceGenes
2000
In vitro selection of an RNA sequence that interacts with high affinity with thymidylate synthase
Lin X, Mizunuma N, Chen T, Copur SM, Maley GF, Liu J, Maley F, Chu E. In vitro selection of an RNA sequence that interacts with high affinity with thymidylate synthase. Nucleic Acids Research 2000, 28: 4266-4274. PMID: 11058126, PMCID: PMC113123, DOI: 10.1093/nar/28.21.4266.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBlotting, WesternColonic NeoplasmsGenes, ReporterHumansMutationNuclease Protection AssaysNucleic Acid ConformationPlasmidsPrecipitin TestsProtein BindingRecombinant Fusion ProteinsReverse Transcriptase Polymerase Chain ReactionRNARNA, MessengerRNA-Binding ProteinsSubstrate SpecificityThymidylate SynthaseTransfectionTumor Cells, CulturedConceptsH630 cellsTS proteinLuciferase reporter constructsRNA sequencesRT-PCRSignificant increaseInduced expressionThymidylate synthaseVivo transfection experimentsCellsSingle RNA moleculesCis-acting elementsHigh-affinity RNAStem-loop structureSecondary structure analysisNovel RNA sequencesDirect bindingHigh affinityReporter constructsTransfection experimentsImmunoprecipitation methodRepressive effectRNA libraries
1998
Cyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain
Kito H, Yokoyama C, Inoue H, Tanabe T, Nakajima N, Sumpio B. Cyclooxygenase Expression in Bovine Aortic Endothelial Cells Exposed to Cyclic Strain. Endothelium 1998, 6: 107-112. PMID: 9930644, DOI: 10.3109/10623329809072197.Peer-Reviewed Original ResearchConceptsAortic endothelial cellsBovine aortic endothelial cellsEndothelial cellsInduction of COXCOX-1 expressionCOX-2 promoter activityExposure of ECsPromoter activityCycles/minCOX-2Cyclooxygenase expressionCOX-1Weak inducerMRNA expressionExtent of inductionNorthern blot analysisBlot analysisTransient transfection experimentsLipopolysaccharideMinimal effectExpressionInductionTransfection experimentsCyclic strainCells
1996
STEP61: A Member of a Family of Brain-Enriched PTPs Is Localized to the Endoplasmic Reticulum
Bult A, Zhao F, Dirkx R, Sharma E, Lukacsi E, Solimena M, Naegele J, Lombroso P. STEP61: A Member of a Family of Brain-Enriched PTPs Is Localized to the Endoplasmic Reticulum. Journal Of Neuroscience 1996, 16: 7821-7831. PMID: 8987810, PMCID: PMC6579237, DOI: 10.1523/jneurosci.16-24-07821.1996.Peer-Reviewed Original ResearchConceptsNovel N-terminal regionEnzymatic activityEndoplasmic reticulumPutative transmembrane domainsIntrinsic membrane proteinsExtended N-terminusAmino acid sequenceN-terminal regionDistinctive intracellular distributionsProtein tyrosineCytosolic variantTransmembrane domainMembrane compartmentsMembrane proteinsAcid sequenceSubcellular fractionationN-terminusRecombinant proteinsTransfection experimentsSTEP61Intracellular distributionProteinReticulumImmunocytochemical labelingFamily
1986
Sorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells.
Gottlieb T, Gonzalez A, Rizzolo L, Rindler M, Adesnik M, Sabatini D. Sorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells. Journal Of Cell Biology 1986, 102: 1242-1255. PMID: 3007530, PMCID: PMC2114186, DOI: 10.1083/jcb.102.4.1242.Peer-Reviewed Original ResearchConceptsG proteinsCell surfaceVesicular stomatitis virusApical plasma membrane domainPlasma membrane domainsPlasma membrane proteinsCell surface domainsApical cell surfaceMadin-Darby canine kidney cellsG protein moleculesViral glycoproteinsLysosome-like vesiclesCanine kidney cellsProtein synthesis inhibitorMembrane domainsMembrane proteinsSurface domainsBasolateral domainEukaryotic expression plasmidTransfection experimentsExpression plasmidProteinPresence of chloroquineApical surfaceProtein molecules
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