2019
A polymorphism in intron I of the human angiotensinogen gene (hAGT) affects binding by HNF3 and hAGT expression and increases blood pressure in mice
Mopidevi B, Kaw MK, Sivankutty I, Jain S, Perla SK, Kumar A. A polymorphism in intron I of the human angiotensinogen gene (hAGT) affects binding by HNF3 and hAGT expression and increases blood pressure in mice. Journal Of Biological Chemistry 2019, 294: 11829-11839. PMID: 31201268, PMCID: PMC6682742, DOI: 10.1074/jbc.ra119.007715.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensinogenAnimalsBinding SitesBlood PressureCCAAT-Enhancer-Binding Protein-betaChromatinHep G2 CellsHepatocyte Nuclear Factor 3-betaHumansIntronsLinkage DisequilibriumLiverMiceMice, Inbred C57BLMice, TransgenicPolymorphism, Single NucleotideProtein BindingReceptors, GlucocorticoidReninRNA, MessengerConceptsIntron IHap-IITransgenic animalsGenome-wide association studiesHepatocyte nuclear factor 3Reporter gene constructsBlood pressureCCAAT enhancer-binding protein βEnhancer-binding protein βHuman angiotensinogen geneStrong homologyHypoxanthine-guanine phosphoribosyltransferase locusNucleotide sequenceGene constructsTranscription rateHuman renin genePromoter activityAssociation studiesHAGT expressionMain haplotypesGenesProtein βElevated blood pressureTransgenic mouse modelFactor 3
2017
Breast cancer-associated gene 3 interacts with Rac1 and augments NF-κB signaling in vitro, but has no effect on RANKL-induced bone resorption in vivo
Yao C, Yu KP, Philbrick W, Sun BH, Simpson C, Zhang C, Insogna K. Breast cancer-associated gene 3 interacts with Rac1 and augments NF-κB signaling in vitro, but has no effect on RANKL-induced bone resorption in vivo. International Journal Of Molecular Medicine 2017, 40: 1067-1077. PMID: 28791343, PMCID: PMC5593463, DOI: 10.3892/ijmm.2017.3091.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBone ResorptionCathepsin KCell LineFemaleFemurFibroblastsGene Expression RegulationHEK293 CellsHeLa CellsHumansMaleMiceMice, Inbred C57BLMice, TransgenicNeuropeptidesNF-kappa BOrgan SpecificityOsteoclastsPromoter Regions, Geneticrac1 GTP-Binding ProteinRANK LigandSignal TransductionTibiaConceptsNF-κB signalingCell type-dependent roleCritical downstream targetNF-κBCanonical NF-κB signalingNuclear factorReceptor activatorNuclear Rac1Adaptor proteinCancer-associated genesMature osteoclast formationSmall GTPaseDownstream targetsExogenous receptor activatorLow-dose RANKLNF-κB interactionTransgenic animalsImportant regulatorBreast cancer-associated genesWild-type littermatesCell typesRac1SignalingBCA3Dependent role
2015
AgRP Neurons Regulate Bone Mass
Kim JG, Sun BH, Dietrich MO, Koch M, Yao GQ, Diano S, Insogna K, Horvath TL. AgRP Neurons Regulate Bone Mass. Cell Reports 2015, 13: 8-14. PMID: 26411686, PMCID: PMC5868421, DOI: 10.1016/j.celrep.2015.08.070.Peer-Reviewed Original ResearchMeSH KeywordsAgouti-Related ProteinAnimalsArcuate Nucleus of HypothalamusBone DensityBone Diseases, MetabolicFemurGene Expression RegulationHomeostasisHypothalamusIon ChannelsLeptinMaleMiceMice, KnockoutMitochondrial ProteinsNeuronsNorepinephrinePhenotypePropranololReceptors, Adrenergic, betaReceptors, LeptinSignal TransductionSirtuin 1TibiaUncoupling Protein 2ConceptsAgRP neuronsCell-autonomous deletionSignificant regulatory roleAgRP neuronal functionBone massLeptin receptor deletionSkeletal bone metabolismTransgenic animalsRegulatory roleGene deletionBone homeostasisDeletionNeuronal functionPostnatal deletionSympathetic toneReceptor deletionArcuate nucleusLeptin actionBone metabolismSkeletal metabolismMultiple linesNeuronsMiceMetabolismCircuit integrity
2014
Hox Complex Analysis Through BAC Recombineering
Parrish M, Ahn Y, Nolte C, De Kumar B, Krumlauf R. Hox Complex Analysis Through BAC Recombineering. Methods In Molecular Biology 2014, 1196: 59-87. PMID: 25151158, DOI: 10.1007/978-1-4939-1242-1_5.Peer-Reviewed Original ResearchHighly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease
Flowers GP, Timberlake AT, Mclean KC, Monaghan JR, Crews CM. Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease. Development 2014, 141: 2165-2171. PMID: 24764077, PMCID: PMC4011087, DOI: 10.1242/dev.105072.Peer-Reviewed Original ResearchMeSH KeywordsAmbystoma mexicanumAnimalsBase SequenceClustered Regularly Interspaced Short Palindromic RepeatsDeoxyribonucleasesEmbryo, NonmammalianGene TargetingGreen Fluorescent ProteinsINDEL MutationMolecular Sequence DataMutagenesis, Site-DirectedRegenerationRNA, Small UntranslatedSequence Homology, Nucleic AcidConceptsUnique regenerative abilityReverse genetics approachUrodele salamandersAxolotl genomeMolecular toolkitCas9 RNADevelopmental phenotypesGenetic approachesNuclease systemLimb regenerationAxolotl Ambystoma mexicanumTransgenic animalsMolecular biologyAmbystoma mexicanumEGFP expressionRegenerative abilityAxolotlRNAMutation frequencySalamandersNucleaseTissue repairIndividual animalsInvaluable insightsVertebrates
2012
Cardiovascular dysregulation of miR‐17‐92 causes a lethal hypertrophic cardiomyopathy and arrhythmogenesis
Danielson LS, Park DS, Rotllan N, Chamorro‐Jorganes A, Guijarro MV, Fernandez‐Hernando C, Fishman GI, Phoon CK, Hernando E. Cardiovascular dysregulation of miR‐17‐92 causes a lethal hypertrophic cardiomyopathy and arrhythmogenesis. The FASEB Journal 2012, 27: 1460-1467. PMID: 23271053, PMCID: PMC3606524, DOI: 10.1096/fj.12-221994.Peer-Reviewed Original ResearchConceptsSmooth muscle tissueHypertrophic cardiomyopathyMiR-17Dose-dependent inductionMuscle tissueCardiovascular dysregulationArrhythmia inducibilityNovel direct targetMicroRNA cluster miR-17Lethal cardiomyopathyPremature mortalityTransgenic heartsMouse modelHeart sizeCluster miR-17CardiomyopathyPrecise mechanismLuciferase assayDirect targetExpression levelsPathological functionsExpression analysisConditional overexpressionTransgenic animalsHeartMouse Transient Global Ischemia Two-Vessel Occlusion Model.
Pontarelli F, Ofengeim D, Zukin RS, Jonas EA. Mouse Transient Global Ischemia Two-Vessel Occlusion Model. Bio-protocol 2012, 2 PMID: 27446974, PMCID: PMC4950949, DOI: 10.21769/bioprotoc.262.Peer-Reviewed Original ResearchMitochondrial channel activityTransient global ischemiaTwo-vessel occlusion modelBcl-2 family proteinsGlobal ischemiaOcclusion modelChannel activityCaspase-resistant formFamily proteinsFour-vessel occlusion modelCaspase activationHuman cardiac arrestNeocortical layers IIHippocampal CA1 neuronsHigh morbidity rateTransgenic animalsBcl-xLRegional strokeHilar neuronsAspiny neuronsFocal ischemiaMorbidity ratePyramidal neuronsCA1 neuronsCardiac arrest
2009
Microinjection of mRNA and morpholino antisense oligonucleotides in zebrafish embryos.
Yuan S, Sun Z. Microinjection of mRNA and morpholino antisense oligonucleotides in zebrafish embryos. Journal Of Visualized Experiments 2009 PMID: 19488022, PMCID: PMC2762915, DOI: 10.3791/1113.Peer-Reviewed Original ResearchConceptsZebrafish embryosOverexpression of EGFPMorpholino oligonucleotide injectionKnockdown of PKD2Microinjection of RNAGerm-line chimerasMicroinjection of mRNACell fate mappingMorpholino microinjectionAutosomal dominant polycystic kidney diseaseGene functionMisexpression studiesMorpholino antisenseMorpholino oligosGene knockdownTransgenic animalsGFP expressionOligonucleotide injectionEGFP mRNAPhenotype analysisEmbryosGene screeningMRNABroad applicationsPKD2Progressive aggregation despite chaperone associations of a mutant SOD1-YFP in transgenic mice that develop ALS
Wang J, Farr GW, Zeiss CJ, Rodriguez-Gil DJ, Wilson JH, Furtak K, Rutkowski DT, Kaufman RJ, Ruse CI, Yates JR, Perrin S, Feany MB, Horwich AL. Progressive aggregation despite chaperone associations of a mutant SOD1-YFP in transgenic mice that develop ALS. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 1392-1397. PMID: 19171884, PMCID: PMC2631083, DOI: 10.1073/pnas.0813045106.Peer-Reviewed Original ResearchConceptsNucleotide exchange factorsIntermediate filament proteinsAbundant cytosolic enzymeChaperone interactionsExchange factorMutant formsAmyotrophic lateral sclerosis resultsChaperone associationMotor neuronsRNA hybridizationTransgenic animalsPunctate aggregatesSuperoxide dismutase 1Filament proteinsCytosolic enzymeMisfolded monomersHuman SOD1Predominant expressionSoluble stateBiochemical analysisInsoluble inclusionsDismutase 1Immunoaffinity captureProteinTransgenic mice
2008
Genetic Models of Alzheimer’s Disease
Mineur Y. Genetic Models of Alzheimer’s Disease. 2008, 539-544. DOI: 10.1007/978-1-59745-285-4_55.Peer-Reviewed Original Research
2006
Stratum-Specific Expression of Human Transferrin Receptor Increases Iron in Mouse Epidermis
Milstone LM, Adams BD, Zhou J, Sanchez V, Shofner J. Stratum-Specific Expression of Human Transferrin Receptor Increases Iron in Mouse Epidermis. Journal Of Investigative Dermatology 2006, 126: 648-652. PMID: 16424878, PMCID: PMC2243218, DOI: 10.1038/sj.jid.5700127.Peer-Reviewed Original ResearchConceptsTransgenic miceStratum-specific expressionTransferrin receptorKeratin 14 promoterTransferrin receptor expressionBody iron lossesReceptor expressionHistological changesSite-dependent differencesLittermate controlsNormal epidermisReceptorsMiceMouse epidermisEpidermal desquamationFerritin expressionHuman transferrin receptorInvolucrin promoterKeratinocytesTransgenic animalsEpidermisAgeExtra ironExpressionAnimals
2005
The Cell-Surface Isoform of Colony Stimulating Factor 1 (CSF1) Restores but Does Not Completely Normalize Fecundity in CSF1-Deficient Mice1
Ovadia S, Insogna K, Yao GQ. The Cell-Surface Isoform of Colony Stimulating Factor 1 (CSF1) Restores but Does Not Completely Normalize Fecundity in CSF1-Deficient Mice1. Biology Of Reproduction 2005, 74: 331-336. PMID: 16237150, DOI: 10.1095/biolreprod.105.045047.Peer-Reviewed Original ResearchConceptsColony stimulating factor 1Viability of offspringReproductive defectsCell surfaceMembrane-bound isoformStimulating factor 1Cell surface isoformAlpha promoterMCSF1Transgenic animalsProteolytic sheddingCSF1 proteinMouse reproductionFactor 1ReproductionTransgenic male miceIsoformsTransgenic miceSperm numberOffspringTissue levelsMurine uterusGenetic absenceControl littermatesPromoterOtx2 Regulates Subtype Specification and Neurogenesis in the Midbrain
Vernay B, Koch M, Vaccarino F, Briscoe J, Simeone A, Kageyama R, Ang SL. Otx2 Regulates Subtype Specification and Neurogenesis in the Midbrain. Journal Of Neuroscience 2005, 25: 4856-4867. PMID: 15888661, PMCID: PMC6724764, DOI: 10.1523/jneurosci.5158-04.2005.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornBasic Helix-Loop-Helix Transcription FactorsBody PatterningBromodeoxyuridineCell CountCell DifferentiationDopamineEmbryo, MammalianEmbryonic InductionFibroblast Growth Factor 8Gene Expression Regulation, DevelopmentalHomeobox Protein Nkx-2.2Homeodomain ProteinsImmunohistochemistryIn Situ HybridizationIn Situ Nick-End LabelingIntermediate Filament ProteinsIntracellular Signaling Peptides and ProteinsKruppel-Like Transcription FactorsMembrane ProteinsMesencephalonMiceMice, TransgenicNerve Tissue ProteinsNestinNeuronsOrganizers, EmbryonicOtx Transcription FactorsPatched ReceptorsReceptors, Cell SurfaceSerotoninTranscription FactorsWnt1 ProteinZebrafish ProteinsZinc Finger Protein GLI1ConceptsMid-hindbrain organizerFunction of Otx2Neuronal subtype identityTranscription factor Otx2Rostral brain developmentEmbryonic day 10.5Cre/loxP systemRole of Otx2Neuronal progenitor cellsMutant embryosProgenitor identityConditional mutantsE10.5 onwardOtx2 activityAnterior hindbrainEctopic expressionCerebellar-like structuresSubtype specificationSubtype identityTransgenic animalsNovel roleLater roleNovel mouse modelOtx2Otx2 expressionA Survivin Gene Signature Predicts Aggressive Tumor Behavior
Salz W, Eisenberg D, Plescia J, Garlick DS, Weiss RM, Wu XR, Sun TT, Altieri DC. A Survivin Gene Signature Predicts Aggressive Tumor Behavior. Cancer Research 2005, 65: 3531-3534. PMID: 15867343, DOI: 10.1158/0008-5472.can-04-4284.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsButylhydroxybutylnitrosamineCarcinogensDisease ProgressionFemaleGene Expression ProfilingGene Expression Regulation, NeoplasticHumansInhibitor of Apoptosis ProteinsMembrane ProteinsMiceMice, TransgenicMicrotubule-Associated ProteinsNeoplasm ProteinsPolymerase Chain ReactionSurvivinTransgenesTumor Suppressor Protein p53Urinary BladderUrinary Bladder NeoplasmsUroplakin IIConceptsTransgenic expressionGene signatureGlobal transcriptional changesDominant negative mutantGene expression profilesSuch genesTumor progressionTranscriptional changesCell divisionEssential regulatorGene expressionExpression profilesTransgenic animalsExtracellular matrixTissue microenvironmentAggressive tumor behaviorPreferential incidenceGenesInflammatory genesSurvivinExpressionTumor behaviorSurvivin expressionAssociated gene signaturesTransgenic modelChapter 20 New Molecular Targets for the Treatment of Neuropathic Pain
Wood J, Waxman S. Chapter 20 New Molecular Targets for the Treatment of Neuropathic Pain. 2005, 339-355. DOI: 10.1016/b978-012738903-5/50021-7.Peer-Reviewed Original ResearchNeuropathic painPeripheral nervous system degenerationGene regulation studiesMolecular targetsAnimal modelsInteresting drug targetGene expressionRegulatory moleculesNew molecular targetsMolecular mechanismsEffective drug developmentNervous system degenerationTransgenic animalsUseful animal modelRegulation studiesDrug targetsGene ablationEfficacy of drugsConsequence of diseaseHerpes zosterImmunodeficiency syndromeRecent insightsPeripheral nervesSystem degenerationPain
2003
Tissue- and agonist-specific regulation of human and murine plasminogen activator inhibitor-1 promoters in transgenic mice
Eren M, Painter CA, Gleaves LA, Schoenhard JA, Atkinson JB, Brown NJ, Vaughan DE. Tissue- and agonist-specific regulation of human and murine plasminogen activator inhibitor-1 promoters in transgenic mice. Journal Of Thrombosis And Haemostasis 2003, 1: 2389-2396. PMID: 14629474, DOI: 10.1046/j.1538-7836.2003.00437.x.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsGene Expression RegulationGreen Fluorescent ProteinsHumansImmunohistochemistryLipopolysaccharidesLuminescent ProteinsMiceMice, TransgenicOrgan SpecificityPlasminogen Activator Inhibitor 1Promoter Regions, GeneticTissue DistributionTransforming Growth Factor betaTransforming Growth Factor beta1ConceptsTranscriptional responseMurine PAI-1Plasminogen activator inhibitor-1 promoterPhysiological regulationPAI-1 promoterPlasminogen activator inhibitor type 1 (PAI-1) expressionGreen fluorescent proteinAgonist-specific regulationTranscription factorsTransgenic strategiesTransgenic miceDNA sequencesPAI-1 expressionHeterologous promoterAng IIQuantitative regulationRegulatory mechanismsRegulatory factorsFluorescent proteinKb promoterTransgenic animalsPhysiological relevancePromoterFunctional studiesEGFP expression
2002
Use of transgenic animals to study renal acid-base transport.
Wang T, Giebisch G, Aronson PS. Use of transgenic animals to study renal acid-base transport. Journal Of Nephrology 2002, 15 Suppl 5: s151-60. PMID: 12027214.Commentaries, Editorials and LettersConceptsAcid-base transportSpecific transporter isoformsSuch knockout miceRenal acid-base transportRenal acid-base homeostasisTransgenic miceTransporter isoformsPump subunitsTransgenic animalsKnockout micePhysiological roleATPase isoformsTransport deficiencyMolecular levelAcid-base homeostasisIsoformsAdaptive mechanismsCarbonic anhydraseCompensatory-adaptive mechanismsUseful experimental modelTransportersRegulatory mediatorsNHE isoformsNitric oxideExperimental model
2000
Introduction of the human growth hormone gene into the guinea pig mammary gland by in vivo transfection promotes sustained expression of human growth hormone in the milk throughout lactation
Hens J, Amstutz M, Schanbacher F, Mather I. Introduction of the human growth hormone gene into the guinea pig mammary gland by in vivo transfection promotes sustained expression of human growth hormone in the milk throughout lactation. Biochimica Et Biophysica Acta 2000, 1523: 161-171. PMID: 11042380, DOI: 10.1016/s0304-4165(00)00117-3.Peer-Reviewed Original ResearchConceptsHuman growth hormone geneGrowth hormone geneGuinea-pig mammary glandPig mammary glandHormone geneBovine mammary cellsTransfection of cellsMammary glandGene promoterBiological roleRecombinant proteinsTransgenic animalsExpression plasmidMammary cellsHuman growth hormoneCytomegalovirus promoterTransfectionSustained expressionGenesPromoterGrowth hormonePlasmid DNAMammary tissueExpressionTransfection complexesRadial Unit Hypothesis of Neocortical Expansion
Rakic P. Radial Unit Hypothesis of Neocortical Expansion. Novartis Foundation Symposia 2000, 228: 30-45. PMID: 10929315, DOI: 10.1002/0470846631.ch3.Peer-Reviewed Original ResearchConceptsRadial unit hypothesisSpecies-specific sizeFamily of genesMutations of genesVentricular zoneMammalian evolutionFounder cellsNatural selectionRegulatory genesCell divisionMorphoregulatory moleculesPostmitotic cellsTransgenic animalsCortical plateCell deathNeocortical expansionGenesBasic organizationGlial scaffoldingColumnar unitsCortical cellsCerebral cortexCellsCortical developmentSynaptic connections
1998
Transgenic Animals with Inducible, Targeted Gene Expression in Brain
Chen J, Kelz M, Zeng G, Sakai N, Steffen C, Shockett P, Picciotto M, Duman R, Nestler E. Transgenic Animals with Inducible, Targeted Gene Expression in Brain. Molecular Pharmacology 1998, 54: 495-503. PMID: 9730908, DOI: 10.1124/mol.54.3.495.Peer-Reviewed Original Research
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