2021
(E)-N-(2-(3, 5-Dimethoxystyryl) phenyl) furan-2-carboxamide (BK3C231) induces cytoprotection in CCD18-Co human colon fibroblast cells through Nrf2/ARE pathway activation
Tan H, Thomas N, Inayat-Hussain S, Chan K. (E)-N-(2-(3, 5-Dimethoxystyryl) phenyl) furan-2-carboxamide (BK3C231) induces cytoprotection in CCD18-Co human colon fibroblast cells through Nrf2/ARE pathway activation. Scientific Reports 2021, 11: 4773. PMID: 33637843, PMCID: PMC7910600, DOI: 10.1038/s41598-021-83163-7.Peer-Reviewed Original ResearchConceptsGlutamate-cysteine ligase catalytic subunitGlutathione S-transferaseNrf2/ARE pathway activationDissociation of Nrf2New mechanistic insightsCatalytic subunitNrf2/ARE pathwayN-furanPromoter activityMolecular mechanismsResponse elementCytoprotective proteinsInhibitory proteinS-transferaseQuinone oxidoreductase 1Mitochondrial damageCytoprotective enzymesMechanistic insightsARE pathwayProtein expressionFactor 2Pathway activationNuclear factorNormal cellsFibroblast cells
2015
Explorative genetic association study of GSTT2B copy number variant in complex disease risks
Iorio A, Polimanti R, Calandro M, Graziano ME, Piacentini S, Bucossi S, Squitti R, Lazzarin N, Scano G, Limbruno GM, Manfellotto D, Fuciarelli M. Explorative genetic association study of GSTT2B copy number variant in complex disease risks. Annals Of Human Biology 2015, 43: 279-284. PMID: 26207597, DOI: 10.3109/03014460.2015.1049206.Peer-Reviewed Original ResearchConceptsOdds ratioAllergic rhinitisEssential hypertensionRecurrent miscarriageAlzheimer's diseaseCopy number variantsGlutathione S-transferaseCase-control groupPhase II enzymesStudy populationItalian patientsLarger sample sizeDisease statusDisease riskExogenous toxic compoundsPhase IPathological phenotypesDiseaseNumber variantsHealth-related phenotypesFurther investigationGenetic association studiesII enzymesComplex diseasesS-transferase
2014
Deletion polymorphism of GSTT1 gene as protective marker for allergic rhinitis
Iorio A, Polimanti R, Piacentini S, Liumbruno GM, Manfellotto D, Fuciarelli M. Deletion polymorphism of GSTT1 gene as protective marker for allergic rhinitis. The Clinical Respiratory Journal 2014, 9: 481-486. PMID: 24902621, DOI: 10.1111/crj.12170.Peer-Reviewed Original ResearchConceptsGlutathione S-transferaseObserved genetic associationAllergic rhinitisGene familyGenomic DNASusceptibility lociXenobiotic compoundsGenesPhase II enzymesS-transferaseCase-control populationII enzymesOxidative stress-related mechanismsComplex diseasesGenetic associationNull polymorphismHealthy non-allergic subjectsHuman populationStress-related mechanismsDeletion polymorphismNon-allergic subjectsCommon respiratory diseasesBiomarkers of inflammationPolymorphismOxidative stressFunctional variability of glutathione S‐transferases in basque populations
Iorio A, Piacentini S, Polimanti R, De Angelis F, Calderon R, Fuciarelli M. Functional variability of glutathione S‐transferases in basque populations. American Journal Of Human Biology 2014, 26: 361-366. PMID: 24677736, DOI: 10.1002/ajhb.22520.Peer-Reviewed Original ResearchConceptsHuman Genome Diversity ProjectGlutathione S-transferaseS-transferaseFunctional variabilityGenetic structureGenetic association studiesCellular detoxificationGenetic variabilityGenome ProjectGST genesAssociation studiesHigh differentiationDiversity ProjectMediterranean populationsGenesCorrespondence analysisBasque populationEnzyme activityHuman populationDNA extractionPCR multiplexWorldwide populationPCR-RFLP methodPolymorphic variantsDrug response
2013
Phenotype versus Genotype Methods for Copy Number Variant Analysis of Glutathione S‐Transferases M1
Piacentini S, Polimanti R, De Angelis F, Iorio A, Fuciarelli M. Phenotype versus Genotype Methods for Copy Number Variant Analysis of Glutathione S‐Transferases M1. Annals Of Human Genetics 2013, 77: 409-415. PMID: 23731058, DOI: 10.1111/ahg.12025.Peer-Reviewed Original ResearchConceptsCopy number variantsGlutathione S-transferaseAnalysis of CNVsHuman populationCopy number variant analysisNumber variant analysisProtein functionSingle nucleotide polymorphismsGenetic association studiesGSTM1 copy number variantAssociation studiesCNV frequencyGenotype methodMissense substitutionsNumber variantsPhenotype analysisGenesNucleotide polymorphismsS-transferaseMissense variantsGenetic associationVariant analysisPhenotypeGSTM1 geneFunctional effectsGlutathione S-transferase polymorphisms, asthma susceptibility and confounding variables: a meta-analysis
Piacentini S, Polimanti R, Simonelli I, Donno S, Pasqualetti P, Manfellotto D, Fuciarelli M. Glutathione S-transferase polymorphisms, asthma susceptibility and confounding variables: a meta-analysis. Molecular Biology Reports 2013, 40: 3299-3313. PMID: 23307299, DOI: 10.1007/s11033-012-2405-2.Peer-Reviewed Original Research
2012
Multiple Insecticide Resistance in Anopheles gambiae s.l. Populations from Burkina Faso, West Africa
Namountougou M, Simard F, Baldet T, Diabaté A, Ouédraogo J, Martin T, Dabiré R. Multiple Insecticide Resistance in Anopheles gambiae s.l. Populations from Burkina Faso, West Africa. PLOS ONE 2012, 7: e48412. PMID: 23189131, PMCID: PMC3506617, DOI: 10.1371/journal.pone.0048412.Peer-Reviewed Original ResearchConceptsGlutathione S-transferaseGambiae populationsInsecticide resistanceNon-specific esterasesResistance phenotypeAce-1 locusInsecticide resistance phenotypeTarget-site mutationsVector populationsAnopheles gambiae populationsMajor malaria vectorMultiple insecticide resistanceMultiple resistance phenotypesPhenotypic diversityMosquito vector populationsKnockdown resistanceResistant allelesS formAnopheles gambiae s.Insecticidal compoundsGambiae mosquitoesSite variationMultiple resistance mechanismsPutative combinationS-transferaseAnaplasma phagocytophilum Asp14 Is an Invasin That Interacts with Mammalian Host Cells via Its C Terminus To Facilitate Infection
Kahlon A, Ojogun N, Ragland SA, Seidman D, Troese MJ, Ottens AK, Mastronunzio JE, Truchan HK, Walker NJ, Borjesson DL, Fikrig E, Carlyon JA. Anaplasma phagocytophilum Asp14 Is an Invasin That Interacts with Mammalian Host Cells via Its C Terminus To Facilitate Infection. Infection And Immunity 2012, 81: 65-79. PMID: 23071137, PMCID: PMC3536139, DOI: 10.1128/iai.00932-12.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnaplasma phagocytophilumAnimalsBacterial Outer Membrane ProteinsBinding SitesCell AdhesionCell Line, TumorEhrlichiaEhrlichiosisGene Expression Regulation, BacterialGlutathione TransferaseHL-60 CellsHumansMembrane GlycoproteinsMembrane ProteinsMiceMolecular Sequence DataProtein BindingProtein Structure, TertiaryProteomeSequence Analysis, ProteinTranscription, GeneticUp-RegulationConceptsMammalian host cellsHost cellsReticulate cellsOuter membrane protein candidatesSurface proteinsOuter membrane protein AMembrane protein AA. phagocytophilum-infected ticksObligate intracellular bacteriumA. phagocytophilumGlutathione S-transferaseTranscriptional profilingSurface proteomeC-terminusAffinity purificationFamily AnaplasmataceaeProtein candidatesSelective biotinylationCellular invasionAsp14Transmission feedingAmino acidsS-transferaseIntracellular bacteriumP-selectin glycoprotein ligand-1Genetic variability of glutathione S-transferase enzymes in human populations: Functional inter-ethnic differences in detoxification systems
Polimanti R, Carboni C, Baesso I, Piacentini S, Iorio A, De Stefano GF, Fuciarelli M. Genetic variability of glutathione S-transferase enzymes in human populations: Functional inter-ethnic differences in detoxification systems. Gene 2012, 512: 102-107. PMID: 23043933, DOI: 10.1016/j.gene.2012.09.113.Peer-Reviewed Original ResearchConceptsGenetic association studiesGenetic variabilityAssociation studiesHuman populationFunctional genetic differencesDetoxification genesGlutathione S-transferaseCellular detoxificationEnzymatic functionGlutathione S-transferase enzymesGST genesBiological processesGenetic differencesDetoxification systemGenesGST enzymesS-transferaseFunctional impactLoF variantsFunction variantsGSTEnzymeHapMap databaseGenetic polymorphismsKey role
2011
GSTO1*E155del polymorphism associated with increased risk for late-onset Alzheimer's disease: Association hypothesis for an uncommon genetic variant
Piacentini S, Polimanti R, Squitti R, Mariani S, Migliore S, Vernieri F, Rossini PM, Manfellotto D, Fuciarelli M. GSTO1*E155del polymorphism associated with increased risk for late-onset Alzheimer's disease: Association hypothesis for an uncommon genetic variant. Neuroscience Letters 2011, 506: 203-207. PMID: 22100662, DOI: 10.1016/j.neulet.2011.11.005.Peer-Reviewed Original ResearchConceptsUncommon genetic variantsGSTO1-1Genetic variantsClass genesGlutathione S-transferaseGenetic variationMultifunctional enzymeCellular detoxificationAlzheimer's diseaseGSTO2 genesGenetic linkageAllele-specific PCRS-transferaseChromosome 10qAD riskGene polymorphismsInterleukin-1β activationSpecific PCRPathophysiology of ADGenesLate-onset Alzheimer's diseasePolymorphismAD risk factorsOxidative stressPCR-RFLPFunctional polymorphisms of GSTA1 and GSTO2 genes associated with asthma in Italian children
Piacentini S, Verrotti A, Polimanti R, Giannini C, Saccucci P, Manfellotto D, Fuciarelli M. Functional polymorphisms of GSTA1 and GSTO2 genes associated with asthma in Italian children. Clinical Chemistry And Laboratory Medicine 2011, 50: 311-315. PMID: 22040239, DOI: 10.1515/cclm.2011.774.Peer-Reviewed Original ResearchConceptsGlutathione S-transferaseAsthma developmentGSTO2 gene polymorphismsSingle nucleotide polymorphismsGenetic variabilityAssociation studiesCellular protectionAntioxidant defense systemGSTO2 genesGSTT1 genotype distributionFunctional polymorphismsNucleotide polymorphismsPathogenesis of asthmaS-transferaseCase-control studyIndependent case-control studiesSusceptible genesDefense systemGSTT1 null genotypeEffect of GSTM1GenesBronchial inflammationGSTA1GSTO2Airway disordersHuman GST Loci as Markers of Evolutionary Forces: GSTO1*E155del and GSTO1*E208K Polymorphisms May Be Under Natural Selection Induced by Environmental Arsenic
Polimanti R, Piacentini S, De Angelis F, De Stefano G, Fuciarelli M. Human GST Loci as Markers of Evolutionary Forces: GSTO1*E155del and GSTO1*E208K Polymorphisms May Be Under Natural Selection Induced by Environmental Arsenic. Disease Markers 2011, 31: 231-239. DOI: 10.1155/2011/124169.Peer-Reviewed Original ResearchGST genesPopulation demographic historyGlutatione S-transferasesAllele frequenciesEvolutionary forcesDemographic historyNatural selectionSelective pressureNull phenotypeNeutral polymorphismsPopulation relationshipsHapMap dataS-transferaseGST SNPsGST lociEnvironmental arsenicGenesWorldwide populationPCR-RFLP methodAfrican populationsPolymorphism
2010
GSTT1 and GSTM1 gene polymorphisms in European and African populations
Piacentini S, Polimanti R, Porreca F, Martínez-Labarga C, De Stefano GF, Fuciarelli M. GSTT1 and GSTM1 gene polymorphisms in European and African populations. Molecular Biology Reports 2010, 38: 1225-1230. PMID: 20563854, DOI: 10.1007/s11033-010-0221-0.Peer-Reviewed Original ResearchConceptsGlutathione S-transferaseGene frequency distributionsAfrican populationsNucleotide substitutionsHuman diseasesSNP analysisAfrica originAllele-specific PCRGene deletionPCR analysisS-transferaseSpecific PCRMultiplex PCR analysisWorldwide populationAllelic frequenciesPharmacogenomic studiesGST variantsImportant insightsPolymorphismSuperfamilyPopulation sampleGSTM1 gene polymorphismsPhenotype frequenciesGene polymorphismsGenes
2000
Human GST Loci as Markers of Evolutionary Forces: GSTO1*E155del and GSTO1*E208K Polymorphisms May Be Under Natural Selection Induced by Environmental Arsenic
Polimanti R, Piacentini S, De Angelis F, De Stefano GF, Fuciarelli M. Human GST Loci as Markers of Evolutionary Forces: GSTO1*E155del and GSTO1*E208K Polymorphisms May Be Under Natural Selection Induced by Environmental Arsenic. Disease Markers 2000, 31: 231-239. PMID: 22045430, PMCID: PMC3826775, DOI: 10.3233/dma-2011-0821.Peer-Reviewed Original ResearchMeSH KeywordsArsenicBiotransformationBlack PeopleEcuadorEnvironmental ExposureEnvironmental PollutantsEvolution, MolecularGene FrequencyGene-Environment InteractionGenetic MarkersGenotypeGlutathione TransferaseHumansIndians, South AmericanLinkage DisequilibriumMalePolymorphism, Single NucleotideSelection, GeneticConceptsGST genesPopulation demographic historyGlutatione S-transferasesAllele frequenciesEvolutionary forcesDemographic historyNatural selectionSelective pressureNull phenotypeNeutral polymorphismsPopulation relationshipsHapMap dataS-transferaseGST SNPsGST lociEnvironmental arsenicGenesWorldwide populationPCR-RFLP methodAfrican populationsPolymorphism
1999
Regulation of Neurabin I Interaction with Protein Phosphatase 1 by Phosphorylation †
McAvoy T, Allen P, Obaishi H, Nakanishi H, Takai Y, Greengard P, Nairn A, Hemmings H. Regulation of Neurabin I Interaction with Protein Phosphatase 1 by Phosphorylation †. Biochemistry 1999, 38: 12943-12949. PMID: 10504266, DOI: 10.1021/bi991227d.Peer-Reviewed Original ResearchConceptsProtein phosphatase 1Neurabin IPP1 activityPhosphatase 1Two-hybrid interaction analysisActin-binding proteinsCo-immunoprecipitation experimentsMimic phosphorylationSerine 461Phosphorylated residuesGlutathione S-transferaseOverlay assaysFusion proteinSignaling mechanismGamma isoformsCAMP pathwayPhosphorylationS-transferaseProteinTryptic digestPKARegulationHPLC-MS analysisInteraction analysisS461
1998
Structure of the Ankyrin-binding Domain of α-Na,K-ATPase*
Zhang Z, Devarajan P, Dorfman A, Morrow J. Structure of the Ankyrin-binding Domain of α-Na,K-ATPase*. Journal Of Biological Chemistry 1998, 273: 18681-18684. PMID: 9668035, DOI: 10.1074/jbc.273.30.18681.Peer-Reviewed Original ResearchConceptsK-ATPaseUbiquitous membrane proteinsSecond cytoplasmic domainSpecific macromolecular interactionsMadin-Darby canine kidney cellsAlpha-NACanine kidney cellsCytoplasmic domainThree-dimensional structureMembrane proteinsGlutathione S-transferasePlasma membraneRegulatory proteinsAnkyrinAmino acids bindFusion proteinResidues 142Trisphosphate receptorVectorial transportSurface loopsMacromolecular interactionsMultiple ankyrinHydrophilic faceS-transferaseFusion peptidePerturbation of Dynamin II with an Amphiphysin SH3 Domain Increases GLUT4 Glucose Transporters at the Plasma Membrane in 3T3-L1 Adipocytes DYNAMIN II PARTICIPATES IN GLUT4 ENDOCYTOSIS*
Volchuk A, Narine S, Foster L, Grabs D, De Camilli P, Klip A. Perturbation of Dynamin II with an Amphiphysin SH3 Domain Increases GLUT4 Glucose Transporters at the Plasma Membrane in 3T3-L1 Adipocytes DYNAMIN II PARTICIPATES IN GLUT4 ENDOCYTOSIS*. Journal Of Biological Chemistry 1998, 273: 8169-8176. PMID: 9525921, DOI: 10.1074/jbc.273.14.8169.Peer-Reviewed Original ResearchConceptsGLUT4 endocytosisDynamin IIGLUT4 glucose transportersPlasma membraneCell surfaceSH3 domainClathrin-coated vesicle formationFusion proteinGlucose transporterLevels of GLUT4Amphiphysin SH3 domainLow-density microsomal fractionIsolated plasma membranesTransferrin endocytosisEndocytic systemGLUT4 internalizationProtein dynaminGlutathione S-transferaseEndosomal compartmentsGLUT4 distributionVesicle formationDynamin peptideEndocytosisGLUT4S-transferase
1996
Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma.
Kuang Y, Wu Y, Smrcka A, Jiang H, Wu D. Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 2964-2968. PMID: 8610151, PMCID: PMC39743, DOI: 10.1073/pnas.93.7.2964.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDBlotting, WesternCell LineChlorocebus aethiopsDNA, ComplementaryGlutamic AcidGTP-Binding ProteinsHumansIsoenzymesKidneyMacromolecular SubstancesMammalsMutagenesis, Site-DirectedPoint MutationPolymerase Chain ReactionReceptor, Anaphylatoxin C5aReceptors, ComplementRecombinant Fusion ProteinsRecombinant ProteinsTransfectionType C PhospholipasesConceptsCOS-7 cellsGlutathione S-transferaseGbeta gammaPLC-beta2Fusion proteinBeta gamma subunitsLigand-induced activationAlpha1B-adrenergic receptorMammalian expression plasmidX boxG proteinsY boxExpression plasmidAmino acidsS-transferaseProteinSequenceResiduesCellsActivationBeta2GalphaGbetaC5a receptorCDNA
1995
The Regulatory Region of Calcium/Calmodulin-dependent Protein Kinase I Contains Closely Associated Autoinhibitory and Calmodulin-binding Domains (∗)
Yokokura H, Picciotto M, Nairn A, Hidaka H. The Regulatory Region of Calcium/Calmodulin-dependent Protein Kinase I Contains Closely Associated Autoinhibitory and Calmodulin-binding Domains (∗). Journal Of Biological Chemistry 1995, 270: 23851-23859. PMID: 7559563, DOI: 10.1074/jbc.270.40.23851.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCalcium-Calmodulin-Dependent Protein Kinase Type 1Calcium-Calmodulin-Dependent Protein KinasesCalmodulinDNA, ComplementaryEnzyme InhibitorsIn Vitro TechniquesMolecular Sequence DataMutagenesisMyosin-Light-Chain KinaseRatsRecombinant Fusion ProteinsSequence DeletionSequence Homology, Amino AcidStructure-Activity RelationshipConceptsCaM kinase IKinase IProtein kinase ITruncation mutantsCalmodulin-dependent protein kinase ICalcium/calmodulin-dependent protein kinase IDependent protein kinase IDependent protein kinaseSyntide-2Active kinaseAutoinhibitory domainDependent activityGlutathione S-transferaseProtein kinaseRegulatory regionsActive mutantMutantsFusion proteinPeptide substratesIntrasteric mechanismGlutathione-Sepharose 4B.COOH-terminalS-transferase
1991
Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent
Berland R, Fikrig E, Rahn D, Hardin J, Flavell RA. Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent. Infection And Immunity 1991, 59: 3531-3535. PMID: 1894359, PMCID: PMC258917, DOI: 10.1128/iai.59.10.3531-3535.1991.Peer-Reviewed Original ResearchConceptsLyme diseaseHumoral responseLate-stage Lyme diseaseEarly humoral responseBorrelia burgdorferiFlagellar antigensPolymerase chain reactionSerological testingImmunodominant domainWestern blotPatientsLyme disease agentAntigenDiseaseChain reactionGlutathione S-transferaseFusion proteinAffinity-purified fusion proteinFlagellin fragmentBacterial flagellinSerumBurgdorferiS-transferaseDisease agentsMolecular characterization
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