2024
A New Chimeric Antibody against the HIV-1 Fusion Inhibitory Peptide MT-C34 with a High Affinity and Fc-Mediated Cellular Cytotoxicity
Kalinichenko S, Ramadan L, Kruglova N, Balagurov K, Lukashina M, Mazurov D, Shepelev M. A New Chimeric Antibody against the HIV-1 Fusion Inhibitory Peptide MT-C34 with a High Affinity and Fc-Mediated Cellular Cytotoxicity. Biology 2024, 13: 675. PMID: 39336102, PMCID: PMC11428423, DOI: 10.3390/biology13090675.Peer-Reviewed Original ResearchCellular cytotoxicityHIV-1Chimeric antibodyInhibitors of HIV-1 entryAntibody-dependent cellular cytotoxicityHIV-1 infectionHIV-1 entryRecombinant chimeric antibodyHumanized antibody trastuzumabMouse monoclonal antibodyCXCR4 locusCD4 lymphocytesCD4 cellsParental mouse monoclonal antibodyCAR cellsGeneration of antibodiesAntibody trastuzumabMonoclonal antibodiesAntibodiesMouse hybridomasConstant regionKnock-inCellsCytotoxicityPeptide
2023
MOGAD patient autoantibodies induce complement, phagocytosis, and cellular cytotoxicity
Yandamuri S, Filipek B, Obaid A, Lele N, Thurman J, Makhani N, Nowak R, Guo Y, Lucchinetti C, Flanagan E, Longbrake E, O’Connor K. MOGAD patient autoantibodies induce complement, phagocytosis, and cellular cytotoxicity. JCI Insight 2023, 8: e165373. PMID: 37097758, PMCID: PMC10393237, DOI: 10.1172/jci.insight.165373.Peer-Reviewed Original ResearchConceptsMyelin oligodendrocyte glycoprotein antibody-associated diseaseAntibody-dependent cellular phagocytosisAntibody-dependent cellular cytotoxicityComplement-dependent cytotoxicityMOG autoantibodiesPatient seraCellular cytotoxicityEffector functionsComplement activityAntibody-associated diseaseMultiple mechanismsNK cellsPatient autoantibodiesCytotoxic capacityLesion histologyCellular phagocytosisFuture relapseIgG subclassesCerebrospinal fluidAutoantibodiesCNS conditionsMOGSerumRelapseCytotoxicity
2022
Double knockout CRISPR screen for cancer resistance to T cell cytotoxicity
Park J, Codina A, Ye L, Lam S, Guo J, Clark P, Zhou X, Peng L, Chen S. Double knockout CRISPR screen for cancer resistance to T cell cytotoxicity. Journal Of Hematology & Oncology 2022, 15: 172. PMID: 36456981, PMCID: PMC9716677, DOI: 10.1186/s13045-022-01389-y.Peer-Reviewed Original ResearchConceptsT cell cytotoxicityCell cytotoxicityT cell killingTumor suppressorCancer patientsImmune responseAvailable agentsSurvival analysisClinical patientsCancer treatmentCancer cellsCancer resistanceDirect targetingPotential new conceptCancer mutationsPatientsCell killingNormal samplesResistance pathwaysCellular responsesSuch resistanceCytotoxicityResistance genesOxidative stress, glutathione, and CYP2E1 in 1,4-dioxane liver cytotoxicity and genotoxicity: insights from animal models
Wang Y, Charkoftaki G, Davidson E, Orlicky D, Tanguay R, Thompson D, Vasiliou V, Chen Y. Oxidative stress, glutathione, and CYP2E1 in 1,4-dioxane liver cytotoxicity and genotoxicity: insights from animal models. Current Opinion In Environmental Science & Health 2022, 29: 100389. PMID: 37483863, DOI: 10.1016/j.coesh.2022.100389.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsOxidative stressUnique mouse modelRelevant low dosesDirect genotoxic effectsLiver cytotoxicityCYP2E1 activationMouse modelAnimal modelsHuman studiesCarcinogenic pathwaysLiver carcinogenicityLow dosesCausal roleGenotoxic effectsHuman exposureUndetermined mechanismPublic healthCarcinogenicityLiver genotoxicityDrinking water contaminantsMechanistic dataGenotoxicityFuture animalCytotoxicityCYP2E1
2021
Endocytosis and the Participation of Glycosaminoglycans Are Important to the Mechanism of Cell Death Induced by β‑Hairpin Antimicrobial Peptides
Buri M, Sperandio L, de Souza K, Antunes F, Rezende M, Melo C, Pinhal M, Barros C, Fernig D, Yates E, Ide J, Smaili S, Riske K, Nader H, dos Santos Tersariol I, Lima M, Judice W, Miranda A, Paredes-Gamero E. Endocytosis and the Participation of Glycosaminoglycans Are Important to the Mechanism of Cell Death Induced by β‑Hairpin Antimicrobial Peptides. ACS Applied Bio Materials 2021, 4: 6488-6501. PMID: 35006908, DOI: 10.1021/acsabm.1c00390.Peer-Reviewed Original ResearchConceptsCell deathAntimicrobial peptidesSecondary structure homologyControl cell deathΒ-hairpin antimicrobial peptidesCommon mechanismCytotoxicity of AMPsStructure homologyMembrane permeabilizationGene expressionMitochondrial potentialCytotoxic modeEndocytosisCell membraneCell tumor modelΒ-hairpin peptidesAMP stimulationPeptidesDynaminHomologyPermeabilizationGlycosaminoglycansMechanismProteinCytotoxicity
2019
A versatile flow-based assay for immunocyte-mediated cytotoxicity
Rabinovich PM, Zhang J, Kerr SR, Cheng BH, Komarovskaya M, Bersenev A, Hurwitz ME, Krause DS, Weissman SM, Katz SG. A versatile flow-based assay for immunocyte-mediated cytotoxicity. Journal Of Immunological Methods 2019, 474: 112668. PMID: 31525367, PMCID: PMC6891822, DOI: 10.1016/j.jim.2019.112668.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorCell NucleusCytotoxicity Tests, ImmunologicCytotoxicity, ImmunologicFlow CytometryHigh-Throughput Screening AssaysHumansImmunotherapy, AdoptiveKiller Cells, NaturalLymphocytes, Tumor-InfiltratingMaleMelanomaMice, Inbred C57BLPredictive Value of TestsReceptors, Chimeric AntigenReproducibility of ResultsSkin NeoplasmsT-LymphocytesTime FactorsWorkflowConceptsCell-mediated cytotoxicityTumor-infiltrating lymphocytesEffector cellsTarget cellsNK-92 cellsChimeric antigen receptorNuclear staining patternInfiltrating lymphocytesT cellsEffector nucleiFlow-based assayImmune systemFlow cytometryStaining patternAntigen receptorDead cellsKilling reactionCytotoxicityCell permeable dyeCellsAssaysCell mixturesNuclear proteinsNovel strategyCell proteinsAssessment of the in vitro toxicity of the disinfection byproduct 2,6-dichloro-1,4-benzoquinone and its transformed derivatives
Hung S, Mohan A, Reckhow DA, Godri Pollitt KJ. Assessment of the in vitro toxicity of the disinfection byproduct 2,6-dichloro-1,4-benzoquinone and its transformed derivatives. Chemosphere 2019, 234: 902-908. PMID: 31519098, DOI: 10.1016/j.chemosphere.2019.06.086.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesNormal human colon cellsHuman liver cancer cellsConcentration-dependent decreaseLiver cancer cellsIntracellular reactive oxygen speciesHuman colon cellsStudy designCell culture mediumCancer cellsColon cellsROS productionCulture mediumGreater cytotoxicityCell linesAcute cytotoxicityCell viabilityToxicological profileToxicological studiesCytotoxicityToxicityEnhanced toxicityOxygen speciesCell mediumPotential underestimation
2018
An early history of T cell-mediated cytotoxicity
Golstein P, Griffiths G. An early history of T cell-mediated cytotoxicity. Nature Reviews Immunology 2018, 18: 527-535. PMID: 29662120, DOI: 10.1038/s41577-018-0009-3.Peer-Reviewed Original Research
2016
Targeting the HSP60/10 chaperonin systems of Trypanosoma brucei as a strategy for treating African sleeping sickness
Abdeen S, Salim N, Mammadova N, Summers CM, Goldsmith-Pestana K, McMahon-Pratt D, Schultz PG, Horwich AL, Chapman E, Johnson SM. Targeting the HSP60/10 chaperonin systems of Trypanosoma brucei as a strategy for treating African sleeping sickness. Bioorganic & Medicinal Chemistry Letters 2016, 26: 5247-5253. PMID: 27720295, DOI: 10.1016/j.bmcl.2016.09.051.Peer-Reviewed Original ResearchConceptsT. brucei infectionBrucei infectionComplex treatment regimensFirst-line drugsNew therapeutic strategiesLeishmania major promastigotesAnti-parasitic potentialTreatment regimensTherapeutic windowTherapeutic strategiesCurrent drugsMajor promastigotesEncouraging initial resultsHuman liverAntibiotic resistanceGreater cytotoxicityProtozoan parasiteKidney cellsMedicinal chemistry optimizationInfectionInhibitorsPotent inhibitorBrucei parasitesDrugsCytotoxicity
2015
Munc18‐2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T‐Lymphocytes
Dieckmann N, Hackmann Y, Aricò M, Griffiths G. Munc18‐2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T‐Lymphocytes. Traffic 2015, 16: 1330-1341. PMID: 26771955, PMCID: PMC4791091, DOI: 10.1111/tra.12337.Peer-Reviewed Original ResearchConceptsCytotoxic T lymphocytesPlasma membraneMunc18-2Granule secretionT lymphocytesMunc18Syntaxin-11Fusion eventsFamilial haemophagocytic lymphohistiocytosisEndogenous localizationSyntaxinImmunological synapseSTX11Cytolytic granulesFHL4Loss of proteinHaemophagocytic lymphohistiocytosisPrimary immunodeficiencyProteinFHL5MembraneSecretionPlasmaLocalizationCytotoxicityEfficacy of Adjuvant 5-Fluorouracil Therapy for Patients with EMAST-Positive Stage II/III Colorectal Cancer
Hamaya Y, Guarinos C, Tseng-Rogenski SS, Iwaizumi M, Das R, Jover R, Castells A, Llor X, Andreu M, Carethers JM. Efficacy of Adjuvant 5-Fluorouracil Therapy for Patients with EMAST-Positive Stage II/III Colorectal Cancer. PLOS ONE 2015, 10: e0127591. PMID: 25996601, PMCID: PMC4440728, DOI: 10.1371/journal.pone.0127591.Peer-Reviewed Original ResearchConceptsColorectal cancerStage II/III CRC patientsStage II/III colorectal cancerKaplan-Meier survival curvesSelected Tetranucleotide RepeatsSporadic colorectal cancerElevated microsatellite alterationsEMAST statusEfficacy of adjuvantsCRC patientsImproved survivalPatient outcomesSomatic dysfunctionPatientsMicrosatellite alterationsSurvival curvesCancerChemotherapySurvival dataSubsequent cytotoxicityEMASTSurvivalMMR functionSame extentCytotoxicity
2014
Involvement of Seladin-1 in goniothalamin-induced apoptosis in urinary bladder cancer cells
Yen H, Fauzi A, Din L, McKelvey-Martin V, Meng C, Inayat-Hussain S, Rajab N. Involvement of Seladin-1 in goniothalamin-induced apoptosis in urinary bladder cancer cells. BMC Complementary Medicine And Therapies 2014, 14: 295. PMID: 25107315, PMCID: PMC4150971, DOI: 10.1186/1472-6882-14-295.Peer-Reviewed Original ResearchConceptsV-FITC/PI labelingPrimary bladder cancerSeladin-1RT4 cellsBladder cancerPI labelingUrinary bladder cancer cellsBladder cancer cellsCell deathBladder cancer cell line RT4Cell line RT4Cancer cell linesConclusionThis studyAlzheimer's diseaseWestern blotCancerCancer cellsMTT assayApoptosisCell linesCancer formationDiseaseCytotoxicityInvolvementDeath
2010
Aplidin as a potential adjunct to radiation therapy: In vitro studies
Rockwell S, Liu Y. Aplidin as a potential adjunct to radiation therapy: In vitro studies. International Journal Of Radiation Biology 2010, 86: 63-70. PMID: 20070217, PMCID: PMC2837581, DOI: 10.3109/09553000903264531.Peer-Reviewed Original ResearchConceptsH drug treatmentDrug treatmentPhase II clinical trialEMT6 mouse mammary tumor cellsGy of radiationMouse mammary tumor cellsMammary tumor cellsBystander effectSupraadditive cytotoxicityTherapeutic regimensHematologic malignanciesClinical trialsRadiation therapyModerate hypoxiaActive metaboliteSevere hypoxiaTumor cellsUntreated cellsTreatmentCytotoxicityHypoxiaAplidinKV X-raysCellsMonolayer cultures
2009
RACK-1 overexpression protects against goniothalamin-induced cell death
Inayat-Hussain S, Wong L, Chan K, Rajab N, Din L, Harun R, Kizilors A, Saxena N, Mourtada-Maarabouni M, Farzaneh F, Williams G. RACK-1 overexpression protects against goniothalamin-induced cell death. Toxicology Letters 2009, 191: 118-122. PMID: 19698770, PMCID: PMC2845802, DOI: 10.1016/j.toxlet.2009.08.012.Peer-Reviewed Original ResearchConceptsRACK-1Cell deathProtein C kinase 1C kinase 1Full-length receptorDNA strand breaksDNA damageStable transfectantsStrand breaksTumor cell linesCell linesOverexpressionT cell linesApoptosisClonogenic assayPotential roleCellsCrucial roleGoniothalaminGenesTransfectantsJurkatPathwayCytotoxic agentsCytotoxicity20 In vitro testing of biomaterials toxicity and biocompatibility
Inayat-Hussain S, Rajab N, Siew E. 20 In vitro testing of biomaterials toxicity and biocompatibility. 2009, 508-537. DOI: 10.1533/9781845695477.3.508.Peer-Reviewed Original Research
2008
Expedient Synthesis of N-Methyl Tubulysin Analogues with High Cytotoxicity
Patterson AW, Peltier HM, Ellman JA. Expedient Synthesis of N-Methyl Tubulysin Analogues with High Cytotoxicity. The Journal Of Organic Chemistry 2008, 73: 4362-4369. PMID: 18479168, DOI: 10.1021/jo800384x.Peer-Reviewed Original ResearchConceptsTubulysin analoguesN-acyl transferExpedient synthesisEfficient synthesisAmide bondN-alkylationTubuvalineStereoselective synthesisOverall yieldO-acylationSynthesisHigher cytotoxicityAnalogue 2Biological studiesHeterocyclesChemistryAnaloguesBondsAminesAcylationLarge quantitiesAlcoholYieldCytotoxicityFormation
2007
Functional inhibition related to structure of a highly potent insulin‐specific CD8 T cell clone using altered peptide ligands
de Marquesini L, Moustakas A, Thomas I, Wen L, Papadopoulos G, Wong F. Functional inhibition related to structure of a highly potent insulin‐specific CD8 T cell clone using altered peptide ligands. European Journal Of Immunology 2007, 38: 240-249. PMID: 18157812, PMCID: PMC2901522, DOI: 10.1002/eji.200737762.Peer-Reviewed Original ResearchConceptsCD8 T cellsT cellsCD8 T cell clonesAntagonist activityT cell functionT cell clonesProduction assaysMHC-peptide complexesTCR contact sitesNOD miceCD8 epitopesAgonist responsesTherapeutic useFunctional inhibitionCell clonesTCR stimulationCell functionPeptide ligandsNative peptideCellsPeptidesCytotoxicityAPLAssaysCD4
2005
Goniothalamin induces apoptosis in vascular smooth muscle cells
Chan K, Rajab N, Ishak M, Ali A, Yusoff K, Din L, Inayat-Hussain S. Goniothalamin induces apoptosis in vascular smooth muscle cells. Chemico-Biological Interactions 2005, 159: 129-140. PMID: 16297902, DOI: 10.1016/j.cbi.2005.10.107.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsSmooth muscle cellsMuscle cellsPathogenesis of restenosisBromodeoxyuridine cell proliferationConcentration-dependent increaseH treatmentCoronary angioplastyMechanism of cytotoxicityVSMCs proliferationDNA damageAbnormal proliferationCell proliferationGN effectApoptotic cellsComet assayRestenosisRapamycinApoptosisCytotoxicityProliferationSimilar effectsMTT cytotoxicityTreatmentCells
1999
Comparison of cytotoxicity and cellular accumulation of polynuclear platinum complexes in L1210 murine leukemia cell lines
Roberts J, Peroutka J, Beggiolin G, Manzotti C, Piazzoni L, Farrell N. Comparison of cytotoxicity and cellular accumulation of polynuclear platinum complexes in L1210 murine leukemia cell lines. Journal Of Inorganic Biochemistry 1999, 77: 47-50. PMID: 10626353, DOI: 10.1016/s0162-0134(99)00137-3.Peer-Reviewed Original ResearchConceptsPolynuclear platinum complexesPlatinum complexesDiamine linkerL1210 cell lineDinuclear platinum complexesTrinuclear platinum complexL1210 murine leukemia cell lineL1210/DDPDinuclear complexesAnticancer profileChemical featuresCytotoxicity profileCellular uptakeBBR3464LinkerMurine leukemia cell lineComplexesTransCharge contributesClinical agentsCytotoxicityUptake pathwayCellular accumulationAntitumor activityPlatinum
1998
IL-18 augments perforin-dependent cytotoxicity of liver NK-T cells.
Dao T, Mehal W, Crispe I. IL-18 augments perforin-dependent cytotoxicity of liver NK-T cells. The Journal Of Immunology 1998, 161: 2217-22. PMID: 9725214, DOI: 10.4049/jimmunol.161.5.2217.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicAnimalsCytokinesCytotoxicity, ImmunologicHumansInterferon InducersInterferon-gammaInterleukin-18Jurkat CellsKiller Cells, NaturalLiverLymphoproliferative DisordersMembrane GlycoproteinsMiceMice, Inbred C57BLMice, Inbred StrainsMice, KnockoutPerforinPore Forming Cytotoxic ProteinsT-Lymphocyte SubsetsT-Lymphocytes, CytotoxicTumor Necrosis Factor-alphaConceptsNK T cellsLiver NK T cellsIL-18NK cellsIntrahepatic lymphocyte subpopulationsNK cell activityPerforin-dependent cytotoxicityPerforin-dependent pathwayTNF-alpha productionSoluble TNF-alphaT cell linesLymphocyte subpopulationsCytotoxic cellsTNF-alphaT cellsCell activityExact mechanismNKCell populationsCell linesLiverCytotoxicityCellsCytokinesCTL
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