2024
Confinement induces internal flows in adherent cell aggregates
Yousafzai M, Amiri S, Sun Z, Pahlavan , Murrell M. Confinement induces internal flows in adherent cell aggregates. Journal Of The Royal Society Interface 2024, 21: 20240105. PMID: 38774959, PMCID: PMC11285874, DOI: 10.1098/rsif.2024.0105.Peer-Reviewed Original Research
2022
Interplay between substrate rigidity and tissue fluidity regulates cell monolayer spreading
Staddon M, Murrell M, Banerjee S. Interplay between substrate rigidity and tissue fluidity regulates cell monolayer spreading. Soft Matter 2022, 18: 7877-7886. PMID: 36205535, PMCID: PMC9700261, DOI: 10.1039/d2sm00757f.Peer-Reviewed Original ResearchConceptsSubstrate rigidityCollective cell motionSubstrate stiffnessTissue fluidityStiff substratesCell-matrix adhesionTraction force generationCell-cell interactionsCell motionTissue morphogenesisSoft elastic matrixCell collectivesEmbryonic developmentCell crawlingCell spreadingCell monolayersSolid tissuesCell behaviorMechanical behaviorPredictive understandingMechanical propertiesCancer invasionCell aggregatesElastic matrixCell properties
2021
Protein Phosphatase 2A as a Therapeutic Target in Small Cell Lung Cancer
Mirzapoiazova T, Xiao G, Mambetsariev B, Nasser MW, Miaou E, Singhal SS, Srivastava S, Mambetsariev I, Nelson MS, Nam A, Behal A, Arvanitis LD, Atri P, Muschen M, Tissot FLH, Miser J, Kovach JS, Sattler M, Batra SK, Kulkarni P, Salgia R. Protein Phosphatase 2A as a Therapeutic Target in Small Cell Lung Cancer. Molecular Cancer Therapeutics 2021, 20: 1820-1835. PMID: 34253596, PMCID: PMC8722383, DOI: 10.1158/1535-7163.mct-21-0013.Peer-Reviewed Original ResearchConceptsProtein phosphatase 2APhosphatase 2ASerine/threonine phosphataseDNA damage responseRegulation of apoptosisSmall molecule inhibitorsGlycolytic ATP productionThreonine phosphataseTwo-dimensional cultureLB100ATP productionMolecule inhibitorsPP2AThree-dimensional spheroid modelEndothelial cell monolayersGlucose uptakeCell viabilitySCLC cellsTherapeutic targetApoptosisCell monolayersMass spectrometrySpheroid modelTumor spheroidsCells
2018
Identification of myocilin as a blood plasma protein and analysis of its role in leukocyte adhesion to endothelial cell monolayers
Aroca-Aguilar JD, Fernández-Navarro A, Ontañón J, Coca-Prados M, Escribano J. Identification of myocilin as a blood plasma protein and analysis of its role in leukocyte adhesion to endothelial cell monolayers. PLOS ONE 2018, 13: e0209364. PMID: 30557320, PMCID: PMC6296516, DOI: 10.1371/journal.pone.0209364.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overBlood ProteinsBlotting, WesternCell AdhesionCytoskeletal ProteinsEye ProteinsFemaleGlycoproteinsHealthy VolunteersHEK293 CellsHuman Umbilical Vein Endothelial CellsHumansLeukocytesLiverMaleMiddle AgedProteolysisReal-Time Polymerase Chain ReactionRNA, MessengerThymus GlandConceptsPresence of myocilinEndothelial cell monolayersWestern immunoblotNon-ocular tissuesCell monolayersLymphoid organsLymphoid tissueT lymphocytesLeukocyte adhesionMatricellular proteinPlasma proteinsHuman myocilinLeukocytesMyocilinSerum proteinsPutative roleQuantitative PCRBlood plasmaLiverBiological activityAnti-adhesive proteinImmunoblotVivo proteolytic processingNew biological propertiesTissue
2016
New Functional Tools for Antithrombogenic Activity Assessment of Live Surface Glycocalyx
Dimitrievska S, Gui L, Weyers A, Lin T, Cai C, Wu W, Tuggle CT, Sundaram S, Balestrini JL, Slattery D, Tchouta L, Kyriakides TR, Tarbell JM, Linhardt RJ, Niklason LE. New Functional Tools for Antithrombogenic Activity Assessment of Live Surface Glycocalyx. Arteriosclerosis Thrombosis And Vascular Biology 2016, 36: 1847-1853. PMID: 27386939, PMCID: PMC5283952, DOI: 10.1161/atvbaha.116.308023.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntithrombinsAorta, ThoracicBiological AssayBlood CoagulationCells, CulturedChromatography, LiquidFactor XaGlycocalyxHeparinHeparitin SulfateHuman Umbilical Vein Endothelial CellsMaleMass SpectrometryMicroscopy, Electron, TransmissionRats, Sprague-DawleyReproducibility of ResultsThrombinTime FactorsConceptsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVein endothelial cellsNative aortaEndothelial cellsHuman umbilical vein endothelial cell monolayersCultured human umbilical vein endothelial cellsFactor XaVascular healthEndothelial cell monolayersVascular diseaseRat aortaDamaged vasculatureAnticoagulant capacityVascular communityAortaTherapeutic developmentActivity assessmentCell monolayersVascular graftsLiquid chromatography-mass spectrometry analysisNovel assayGlycocalyxAssaysNative rat aorta
2014
Polarized Granzyme Release Is Required for Antigen-Driven Transendothelial Migration of Human Effector Memory CD4 T Cells
Manes TD, Pober JS. Polarized Granzyme Release Is Required for Antigen-Driven Transendothelial Migration of Human Effector Memory CD4 T Cells. The Journal Of Immunology 2014, 193: 5809-5815. PMID: 25367116, PMCID: PMC4258477, DOI: 10.4049/jimmunol.1401665.Peer-Reviewed Original ResearchConceptsEffector memory CD4 T cellsMemory CD4 T cellsCD4 T cellsT cellsTransendothelial migrationImmune synapse formationEndothelial cell monolayersInflammatory chemokinesMorphologic manifestationsGranzyme releaseSynapse formationTCR recognitionGranule dischargeDynein-driven transportChemokinesCell monolayersEarly eventsEndotheliumCytosolic granulesCellsMTOC reorientationProteolytic activityMicrotubule organizing centerMolecular requirementsGranule function
2006
PECAM‐1: a multi‐faceted regulator of megakaryocytopoiesis
Wu Y, Madri J. PECAM‐1: a multi‐faceted regulator of megakaryocytopoiesis. The FASEB Journal 2006, 20: a633-a633. DOI: 10.1096/fasebj.20.4.a633-c.Peer-Reviewed Original ResearchNull animalsMulti-faceted regulatorAltered localizationActin filament organizationProgenitor cellsDifferentiation of megakaryocytesCommitted progenitor cellsEndothelial cell monolayersQuiescent G0 phaseHematopoietic stem cellsSHP-2Progenitor populationsMarrow progenitor cellsSHP-1Filament organizationPECAM-1Mature megakaryocytesCell monolayersFGF-4Colony formation potentialStem cellsAbnormal megakaryocytopoiesisMegakaryocyte adhesionG0 phaseLSK population
1999
Chronic in vitro shear stress stimulates endothelial cell retention on prosthetic vascular grafts and reduces subsequent in vivo neointimal thickness
Dardik A, Liu A, Ballermann B. Chronic in vitro shear stress stimulates endothelial cell retention on prosthetic vascular grafts and reduces subsequent in vivo neointimal thickness. Journal Of Vascular Surgery 1999, 29: 157-167. PMID: 9882800, DOI: 10.1016/s0741-5214(99)70357-5.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsbeta-GalactosidaseBlood Vessel ProsthesisCell AdhesionCells, CulturedEndothelium, VascularFemaleGenetic VectorsHemorheologyHemostasisIn Vitro TechniquesMicroscopy, Electron, ScanningPolyurethanesRatsRats, Inbred F344Stress, MechanicalThrombosisTransfectionTunica IntimaConceptsProsthetic vascular graftsNeointimal thicknessDyne/cm2 shear stressEndothelial cellsGraft thrombosisEndothelial cell monolayersEndothelial cell retentionHemostasis timeVascular graftsCell monolayersAlpha-smooth muscle actinFischer 344 rat aortaGraft patency rateReplication-deficient adenoviral vectorLuminal surfaceAortic interposition graftsImmediate graft thrombosisChronic shear stressCultured endothelial cellsGraft failureInterposition graftMacrophage infiltrationPatency ratesCell-seeded graftsRat aorta
1993
Fibronectin expression correlates with U937 cell adhesion to migrating bovine aortic endothelial cells in vitro.
Hauser I, Setter E, Bell L, Madri J. Fibronectin expression correlates with U937 cell adhesion to migrating bovine aortic endothelial cells in vitro. American Journal Of Pathology 1993, 143: 173-80. PMID: 7686342, PMCID: PMC1886955.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell AdhesionCell MovementCells, CulturedDrug ResistanceEndothelium, VascularFibronectinsFluorescent Antibody TechniqueHumansIntegrinsLeukemia, Promyelocytic, AcuteNeomycinProto-Oncogene MasProto-Oncogene Proteins pp60(c-src)Transforming Growth Factor betaTumor Cells, CulturedConceptsCell adhesionU937 cell adhesionEndothelial cell populationSRC proto-oncogeneEndothelial cellsCell populationsExtracellular matrix componentsEndothelial cell migrationBovine aortic endothelial cellsExtracellular matrix synthesisDenudation injuryFibronectin depositionCell migrationProto-oncogeneVascular endothelial cellsU937 cellsAortic endothelial cellsAmount of fibronectinMatrix componentsMigratory responseEndothelial cell monolayersMatrix synthesisCellsCell monolayersAdhesionPhotoinhibition of smooth muscle cell migration: Potential therapy for restenosis
Deckelbaum L, Scott J, Stetz M, O'Brien K, Sumpio B, Madri J, Bell L. Photoinhibition of smooth muscle cell migration: Potential therapy for restenosis. Lasers In Surgery And Medicine 1993, 13: 4-11. PMID: 8426525, DOI: 10.1002/lsm.1900130104.Peer-Reviewed Original ResearchConceptsSmooth muscle cell migrationMuscle cell migrationLow energy light irradiationNeointima formationBroadband white lightCell migrationLight irradiationIncidence of restenosisLuminal cross-sectional areaSmooth muscle cell monolayersSmooth muscle cellsFibrointimal thickeningArterial injuryPotential therapyDaily radiant exposureRadiant exposureMuscle cellsThymidine incorporationRestenosisCross-sectional areaMigration of cellsCell proliferationCell monolayersMigration kineticsWhite light
1990
Influence of the angiotensin system on endothelial and smooth muscle cell migration.
Bell L, Madri J. Influence of the angiotensin system on endothelial and smooth muscle cell migration. American Journal Of Pathology 1990, 137: 7-12. PMID: 2164777, PMCID: PMC1877705.Peer-Reviewed Original ResearchConceptsSmooth muscle cell migrationVessel wall responseMuscle cell migrationBovine aortic endothelial cellsAortic smooth muscle cell migrationMedial smooth muscle cellsEndothelial cellsAngiotensin system componentsIle8-angiotensin IIEnzyme inhibitor lisinoprilSmooth muscle cellsCell migrationAortic endothelial cellsAngiotensin systemAngiotensin-converting enzyme inhibitor lisinoprilInhibitor lisinoprilLuminal sizeVessel functionMuscle cellsCell monolayersInjuryCultured cell monolayersPresent studyImportant determinantCells
1989
Evaluation of immunofluorescent reagents, centrifugation, and conventional cultures for the diagnosis of adenovirus infection
Mahafzah A, Landry M. Evaluation of immunofluorescent reagents, centrifugation, and conventional cultures for the diagnosis of adenovirus infection. Diagnostic Microbiology And Infectious Disease 1989, 12: 407-411. PMID: 2558834, DOI: 10.1016/0732-8893(89)90111-9.Peer-Reviewed Original ResearchConceptsIdentification of adenovirusCentrifugation cultureA549 cellsConventional cultureIsolation of adenovirusA549 cell monolayersMRC-5 cellsAdenovirus infectionRapid diagnosisMonoclonal antibodiesGroup-reactive antigenCell monolayersFluorescent-antibody reagentsAdenovirusAntibody reagentsDiagnosisDaysCellsImmunofluorescent reagentsHEKEVIDENCE THAT CYTOTOXIC LYMPHOCYTES ALTER AND TRAVERSE ALLOGENEIC ENDOTHELIAL CELL MONOLAYERS
BENDER J, PARDI R, KOSEK J, ENGLEMAN E. EVIDENCE THAT CYTOTOXIC LYMPHOCYTES ALTER AND TRAVERSE ALLOGENEIC ENDOTHELIAL CELL MONOLAYERS. Transplantation 1989, 47: 1047-1052. PMID: 2660343, DOI: 10.1097/00007890-198906000-00026.Peer-Reviewed Original ResearchConceptsAllogeneic endothelial cellsEndothelial cellsClass II major histocompatibility complex antigensMajor histocompatibility complex antigensLymphocyte-EC adhesionHistocompatibility complex antigensEC monolayersSame morphologic changesAllogeneic lymphocytesLymphocyte subsetsLymphocyte subpopulationsAutologous combinationsEndothelial cell monolayersSuch lymphocytesSurface phenotypeComplex antigensIntact EC monolayerRecombinant cytokinesMorphologic changesLymphocytesCell monolayersEC barrierFunctional consequencesCurrent findingsCellular interactions
1984
Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells.
Bevilacqua MP, Pober JS, Majeau GR, Cotran RS, Gimbrone MA. Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells. Journal Of Experimental Medicine 1984, 160: 618-623. PMID: 6332168, PMCID: PMC2187463, DOI: 10.1084/jem.160.2.618.Peer-Reviewed Original ResearchConceptsEndothelial cell procoagulant activityCell procoagulant activityProcoagulant activityInterleukin-1Cultured human vascular endotheliumHuman monocyte-derived interleukin 1Coagulation factor-deficient plasmasMonocyte-derived interleukin 1Human vascular endothelial cellsHuman vascular endotheliumVascular endothelial cellsFactor-deficient plasmaIL-1 inductionIntravascular coagulationInflammatory conditionsCell surface expressionIntact cell monolayersVascular endotheliumEndothelial cellsPotent inducerSurface expressionCell monolayersProtein synthesisPathogenesisActivity
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