Contribution of individual histidines to the global stability of human prolactin
Keeler C, Tettamanzi MC, Meshack S, Hodsdon ME. Contribution of individual histidines to the global stability of human prolactin. Protein Science 2009, 18: 909-920. PMID: 19384991, PMCID: PMC2771294, DOI: 10.1002/pro.100.Peer-Reviewed Original ResearchConceptsIndividual histidineClosest evolutionary cousinsEvolutionary roleDouble mutant cyclesHomologous residuesEvolutionary cousinsNearby histidineExtracellular domainNative proteinTertiary structureStability of hGHNative stateMutant cyclesHuman growth hormoneHistidineStructural interactionsHuman prolactinResiduesPolypeptide hormonesPhysiologic pH rangeFunctional propertiesStructural locationAffinityProteinMutations