2009
Contribution of individual histidines to the global stability of human prolactin
Keeler C, Tettamanzi MC, Meshack S, Hodsdon ME. Contribution of individual histidines to the global stability of human prolactin. Protein Science 2009, 18: 909-920. PMID: 19384991, PMCID: PMC2771294, DOI: 10.1002/pro.100.Peer-Reviewed Original ResearchConceptsIndividual histidineClosest evolutionary cousinsEvolutionary roleDouble mutant cyclesHomologous residuesEvolutionary cousinsNearby histidineExtracellular domainNative proteinTertiary structureStability of hGHNative stateMutant cyclesHuman growth hormoneHistidineStructural interactionsHuman prolactinResiduesPolypeptide hormonesPhysiologic pH rangeFunctional propertiesStructural locationAffinityProteinMutations
2008
Analysis of Site-Specific Histidine Protonation in Human Prolactin ,
Tettamanzi MC, Keeler C, Meshack S, Hodsdon ME. Analysis of Site-Specific Histidine Protonation in Human Prolactin ,. Biochemistry 2008, 47: 8638-8647. PMID: 18652486, PMCID: PMC2766358, DOI: 10.1021/bi800444t.Peer-Reviewed Original ResearchConceptsPH-dependent regulationSite-directed mutagenesisExtracellular domainHistidine residuesHistidine protonationStatistical supportHPRL receptorFunctional propertiesHuman prolactinProtein surfaceBiophysical mechanismsBiophysical originApparent midpointEquilibrium dissociation constantsHistidineResiduesProtein hormonesDetailed understandingCooperativity constantsSurprising numberDissociation rateMutagenesisDissociation constantsProteinNMR spectroscopy
2002
Aggregation of Human Wild-Type and H27A-Prolactin in Cells and in Solution: Roles of Zn2+, Cu2+, and pH
Sankoorikal B, Zhu Y, Hodsdon M, Lolis E, Dannies P. Aggregation of Human Wild-Type and H27A-Prolactin in Cells and in Solution: Roles of Zn2+, Cu2+, and pH. Endocrinology 2002, 143: 1302-1309. DOI: 10.1210/en.143.4.1302.Peer-Reviewed Original ResearchAtT20 cellsSecretory granulesFormation of secretory granulesHuman prolactinZn2+-binding siteTransfected AtT20 cellsCores of secretory granulesHuman wild-typeWild-type prolactinAcidic pHCOS cellsRecombinant human prolactinIntracellular compartmentsWild-typeBinding sitesRat prolactinCu2+Neutral pHProlactinRatio of Zn2Concentrations of hormonesAtT20Cells