2021
Bone Marrow-Derived VSELs Engraft as Lung Epithelial Progenitor Cells after Bleomycin-Induced Lung Injury
Ciechanowicz AK, Sielatycka K, Cymer M, Skoda M, Suszyńska M, Bujko K, Ratajczak MZ, Krause DS, Kucia M. Bone Marrow-Derived VSELs Engraft as Lung Epithelial Progenitor Cells after Bleomycin-Induced Lung Injury. Cells 2021, 10: 1570. PMID: 34206516, PMCID: PMC8303224, DOI: 10.3390/cells10071570.Peer-Reviewed Original ResearchConceptsBronchioalveolar stem cellsOrganoid assaysAT2 cellsStem cellsH2B-GFP fusion proteinLung epithelial progenitor cellsProgenitor cellsEmbryonic-like stem cellsSurfactant protein CSmall embryonic-like stem cellsEpithelial progenitor cellsLung injuryNonhematopoietic stem cellsFusion proteinAlveolar type 2 cellsPhysiological potentialProgenitor activityBleomycin-Induced Lung InjuryH2B-GFP miceWT recipient miceRegenerative functionSPC promoterType 2 cellsVSELsReporter mice
2019
MKL1-actin pathway restricts chromatin accessibility and prevents mature pluripotency activation
Hu X, Liu ZZ, Chen X, Schulz VP, Kumar A, Hartman AA, Weinstein J, Johnston JF, Rodriguez EC, Eastman AE, Cheng J, Min L, Zhong M, Carroll C, Gallagher PG, Lu J, Schwartz M, King MC, Krause DS, Guo S. MKL1-actin pathway restricts chromatin accessibility and prevents mature pluripotency activation. Nature Communications 2019, 10: 1695. PMID: 30979898, PMCID: PMC6461646, DOI: 10.1038/s41467-019-09636-6.Peer-Reviewed Original ResearchConceptsCell fate reprogrammingChromatin accessibilityActin cytoskeletonSomatic cell reprogrammingPluripotency transcription factorsGlobal chromatin accessibilityGenomic accessibilityCytoskeleton (LINC) complexCell reprogrammingCytoskeletal genesTranscription factorsReprogrammingPluripotencyChromatinCytoskeletonMKL1Unappreciated aspectPathwayNuclear volumeNucleoskeletonSUN2CellsActivationGenesExpression
2008
Hepatocyte Nuclear Factor‐1 as Marker of Epithelial Phenotype Reveals Marrow‐Derived Hepatocytes, but Not Duct Cells, After Liver Injury in Mice
Swenson ES, Guest I, Ilic Z, Mazzeo‐Helgevold M, Lizardi P, Hardiman C, Sell S, Krause DS. Hepatocyte Nuclear Factor‐1 as Marker of Epithelial Phenotype Reveals Marrow‐Derived Hepatocytes, but Not Duct Cells, After Liver Injury in Mice. Stem Cells 2008, 26: 1768-1777. PMID: 18467658, PMCID: PMC2846397, DOI: 10.1634/stemcells.2008-0148.Peer-Reviewed Original ResearchConceptsMarrow-derived epithelial cellsHepatocyte nuclear factor 1Y chromosomeNuclear factor 1Ductal progenitor cellsLiver injuryInflammatory cellsFemale miceProgenitor cellsEpithelial cellsFactor 1Male bone marrowStable hematopoietic engraftmentBone marrow originColocalization of GFPNuclear markersBone marrow cellsDuctal progenitorsHematopoietic engraftmentChromosomesBone marrowMarrow originPancytokeratin stainingCholangiocyte phenotypeMarrow cells
2007
Limitations of Green Fluorescent Protein as a Cell Lineage Marker
Swenson ES, Price JG, Brazelton T, Krause DS. Limitations of Green Fluorescent Protein as a Cell Lineage Marker. Stem Cells 2007, 25: 2593-2600. PMID: 17615263, DOI: 10.1634/stemcells.2007-0241.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood CellsCattleCell LineageChickensCytomegalovirusFemaleFluorescent Antibody TechniqueGene ExpressionGenes, ReporterGenes, SyntheticGreen Fluorescent ProteinsHumansImmunoenzyme TechniquesMaleMiceMice, Inbred C57BLOrgan SpecificityRegulatory Sequences, Nucleic AcidTissue DistributionTransgenesVisceraConceptsSmall intestineMouse strainsPeripheral blood cellsTransgenic mouse strainReporter mouse strainPrimary rabbit antibodiesDonor originHuman ubiquitin C promoterImmunohistochemical stainingSolid organsCell lineage markersCell lineagesUBC-GFP miceUbiquitin C promoterChicken beta-actinFlow cytometryBlood cellsEnhanced green fluorescent protein (EGFP) reporterMiceOne-tissueAdult liverTissue sectionsIntestineLineage markersRabbit antibodiesThe commonly used β-actin-GFP transgenic mouse strain develops a distinct type of glomerulosclerosis
Guo JK, Cheng EC, Wang L, Swenson ES, Ardito TA, Kashgarian M, Cantley LG, Krause DS. The commonly used β-actin-GFP transgenic mouse strain develops a distinct type of glomerulosclerosis. Transgenic Research 2007, 16: 829-834. PMID: 17594530, DOI: 10.1007/s11248-007-9107-x.Peer-Reviewed Original Research
2005
Integration of engrafted Schwann cells into injured peripheral nerve: Axonal association and nodal formation on regenerated axons
Radtke C, Akiyama Y, Lankford KL, Vogt PM, Krause DS, Kocsis JD. Integration of engrafted Schwann cells into injured peripheral nerve: Axonal association and nodal formation on regenerated axons. Neuroscience Letters 2005, 387: 85-89. PMID: 16084645, PMCID: PMC2605373, DOI: 10.1016/j.neulet.2005.06.073.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxotomyCell Adhesion Molecules, NeuronalCell CompartmentationCytoplasmDisease Models, AnimalFemaleGreen Fluorescent ProteinsImmunohistochemistryMaleMiceMice, Inbred C57BLMicroscopy, Electron, TransmissionMyelin SheathNAV1.6 Voltage-Gated Sodium ChannelNerve RegenerationNerve Tissue ProteinsPeripheral Nerve InjuriesPeripheral NervesRanvier's NodesSchwann CellsSciatic NeuropathySodium ChannelsY ChromosomeConceptsWild-type miceSchwann cellsMyelin-forming cellsRegenerated axonsSodium channelsType miceRegenerated peripheral nerve fibersFemale wild-type miceDonor cellsMale donor cellsPeripheral nerve fibersSciatic nerve axonsImmuno-electron microscopic analysisCrush injuryCrush sitePeripheral nervesDonor originMale miceNerve fibersNerve axonsNodal formationAxonsNerveMiceAxonal associations
2004
Bone Marrow-Derived Cells Contribute to Epithelial Engraftment during Wound Healing
Borue X, Lee S, Grove J, Herzog EL, Harris R, Diflo T, Glusac E, Hyman K, Theise ND, Krause DS. Bone Marrow-Derived Cells Contribute to Epithelial Engraftment during Wound Healing. American Journal Of Pathology 2004, 165: 1767-1772. PMID: 15509544, PMCID: PMC1618655, DOI: 10.1016/s0002-9440(10)63431-1.Peer-Reviewed Original ResearchConceptsBone marrow-derived cellsEpithelial cellsMarrow-derived epithelial cellsEngraftment of BMDCsDonor-derived cellsMarrow-derived cellsWound healingDegree of engraftmentLevel of engraftmentAbsence of injuryEarly wound healingFemale miceBone marrowCytokeratin 5Cre-lox systemEngraftmentSkin damageWound edgeKeratinocytesInjuryWound siteTransit-amplifying cellsStem cellsEpidermal stem cellsRecent findingsLack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia
Harris RG, Herzog EL, Bruscia EM, Grove JE, Van Arnam JS, Krause DS. Lack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia. Science 2004, 305: 90-93. PMID: 15232107, DOI: 10.1126/science.1098925.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GalactosidaseBone Marrow CellsBone Marrow TransplantationCell DifferentiationCell FusionCobra Cardiotoxin ProteinsElapid VenomsEpithelial CellsFemaleGreen Fluorescent ProteinsHepatocytesKeratinocytesKeratinsLuminescent ProteinsMaleMiceMice, TransgenicMuscle CellsRadiation, IonizingRecombinasesRecombination, GeneticReverse Transcriptase Polymerase Chain ReactionStem CellsX ChromosomeY ChromosomeConceptsCell-cell fusionBone marrow-derived cellsCre/lox systemGreen fluorescent protein expressionFluorescent protein expressionEpithelial cellsDevelopmental plasticityLox systemCell fusionProtein expressionMarrow-derived cellsTransgenic miceCellsBone marrowFusionFusion requirementsPlasticityExpression
2002
Marrow-Derived Cells as Vehicles for Delivery of Gene Therapy to Pulmonary Epithelium
Grove JE, Lutzko C, Priller J, Henegariu O, Theise ND, Kohn DB, Krause DS. Marrow-Derived Cells as Vehicles for Delivery of Gene Therapy to Pulmonary Epithelium. American Journal Of Respiratory Cell And Molecular Biology 2002, 27: 645-651. PMID: 12444022, DOI: 10.1165/rcmb.2002-0056rc.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Marrow TransplantationFemaleGene ExpressionGenetic TherapyGreen Fluorescent ProteinsHematopoietic Stem Cell TransplantationIndicators and ReagentsLuminescent ProteinsLung DiseasesMiceMice, Inbred C57BLProtein PrecursorsProteolipidsRespiratory MucosaRetroviridaeRNA, MessengerConceptsBone marrow-derived stem cellsLung epithelial cellsLung epitheliumEpithelial cellsMarrow-derived stem cellsGene therapyBMSC transplantationLung diseaseFemale miceAirway epitheliumAlveolar spaceLong-term transgene expressionMale marrowPulmonary epitheliumLong-term gene expressionGene therapy applicationsGene expressionPulmonary airwaysAdenoviral vectorEpitheliumMarrowTherapyTransient gene expressionNonviral vectorsStem cells