2010
SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis
Yu L, Ji W, Zhang H, Renda MJ, He Y, Lin S, Cheng EC, Chen H, Krause DS, Min W. SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis. Journal Of Experimental Medicine 2010, 207: 1183-1195. PMID: 20457756, PMCID: PMC2882842, DOI: 10.1084/jem.20092215.Peer-Reviewed Original ResearchConceptsSmall ubiquitin-like modifier (SUMO) modificationImportant regulatory mechanismEmbryonic day 13.5Down-regulation correlatesFetal liverCre-loxP systemEmbryonic lethalityProtein functionDefinitive erythropoiesisGene promoterDNA bindingRegulatory mechanismsGene expressionGATA1SENP1Fetal liver cellsProtein analysisDay 13.5Global deletionProteinSubsequent erythropoiesisKnockout miceErythropoiesisLiver cellsDeSUMOylation
2009
Role for MKL1 in megakaryocytic maturation
Cheng EC, Luo Q, Bruscia EM, Renda MJ, Troy JA, Massaro SA, Tuck D, Schulz V, Mane SM, Berliner N, Sun Y, Morris SW, Qiu C, Krause DS. Role for MKL1 in megakaryocytic maturation. Blood 2009, 113: 2826-2834. PMID: 19136660, PMCID: PMC2661865, DOI: 10.1182/blood-2008-09-180596.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood Cell CountBone MarrowCell DifferentiationCell Line, TumorCells, CulturedDNA-Binding ProteinsGene Expression ProfilingGene Expression RegulationHumansLeukemia, Erythroblastic, AcuteMegakaryocytesMiceMice, Inbred C57BLMice, KnockoutOligonucleotide Array Sequence AnalysisOncogene Proteins, FusionPloidiesRecombinant Fusion ProteinsRNA InterferenceRNA, Small InterferingSerum Response FactorThrombocytopeniaThrombopoiesisThrombopoietinTrans-ActivatorsConceptsMegakaryoblastic leukemia 1Reduced platelet countsSerum response factorMegakaryocytic differentiationPeripheral bloodPlatelet countMKL1 expressionMegakaryoblastic leukemiaBone marrow megakaryocytesMuscle cellsPresence of thrombopoietinPhysiologic maturationHuman erythroleukemia cell lineIncreased numberMarrow megakaryocytesCell linesErythroleukemia cell lineMegakaryocytesMegakaryocytic maturationDifferentiated muscle cellsOverexpressionConcurrent increaseMuscle differentiationCellsMaturation
2000
Derivation of hepatocytes from bone marrow cells in mice after radiation‐induced myeloablation
Theise N, Badve S, Saxena R, Henegariu O, Sell S, Crawford J, Krause D. Derivation of hepatocytes from bone marrow cells in mice after radiation‐induced myeloablation. Hepatology 2000, 31: 235-240. PMID: 10613752, DOI: 10.1002/hep.510310135.Peer-Reviewed Original ResearchConceptsBone marrow cellsY chromosomeMarrow cellsFemale miceMessenger RNAWhole bone marrow transplantsAge-matched male donorsDerivation of hepatocytesSkeletal muscle regenerationSevere acute injuryAcute hepatic injuryBone marrow transplantationBone marrow transplantSimultaneous FISHFluorescence-activated cell sorterMale-derived cellsOval cell proliferationDays posttransplantationMonths posttransplantationHepatic injuryMarrow transplantationMarrow transplantAcute injuryMonth 2Hepatic engraftment