2018
The interaction between IKKα and LC3 promotes type I interferon production through the TLR9-containing LAPosome
Hayashi K, Taura M, Iwasaki A. The interaction between IKKα and LC3 promotes type I interferon production through the TLR9-containing LAPosome. Science Signaling 2018, 11 PMID: 29717061, PMCID: PMC6462218, DOI: 10.1126/scisignal.aan4144.Peer-Reviewed Original ResearchConceptsInterferon regulatory factor 7Autophagy protein LC3Type I IFN productionI interferonI IFN productionMicrotubule-associated proteinsPutative LC3Type I interferon productionEndosomal vesiclesAutophagosome formationNoncanonical autophagyToll-like receptor 9Production of IFNStimulation of TLR9Regulatory factor 7Protein LC3Direct bindingI interferon productionIFN productionEndosomesChain 3Type I interferonKinase IKKαLC3Lap formation
2017
Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense
Sasai M, Sakaguchi N, Ma JS, Nakamura S, Kawabata T, Bando H, Lee Y, Saitoh T, Akira S, Iwasaki A, Standley DM, Yoshimori T, Yamamoto M. Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense. Nature Immunology 2017, 18: 899-910. PMID: 28604719, DOI: 10.1038/ni.3767.Peer-Reviewed Original ResearchADP-Ribosylation Factor 1AnimalsApoptosis Regulatory ProteinsAutophagyAutophagy-Related Protein 8 FamilyCarrier ProteinsComputer SimulationCRISPR-Cas SystemsCytoskeletal ProteinsEnzyme-Linked Immunosorbent AssayFlow CytometryFluorescent Antibody TechniqueGene EditingGTP PhosphohydrolasesImmunoblottingImmunoprecipitationInterferon-gammaIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceMicrotubule-Associated ProteinsToxoplasmaToxoplasmosis
2012
Noncanonical Autophagy Is Required for Type I Interferon Secretion in Response to DNA-Immune Complexes
Henault J, Martinez J, Riggs JM, Tian J, Mehta P, Clarke L, Sasai M, Latz E, Brinkmann MM, Iwasaki A, Coyle AJ, Kolbeck R, Green DR, Sanjuan MA. Noncanonical Autophagy Is Required for Type I Interferon Secretion in Response to DNA-Immune Complexes. Immunity 2012, 37: 986-997. PMID: 23219390, PMCID: PMC3786711, DOI: 10.1016/j.immuni.2012.09.014.Peer-Reviewed Original ResearchConceptsToll-like receptor 9Adaptor protein 3Plasmacytoid dendritic cellsDNA-containing immune complexesI interferonPathogenic DNAMicrotubule-associated proteinsType I interferon secretionIFN-α productionDNA immune complexesPreinitiation complexType I interferonAnti-DNA autoantibodiesNoncanonical autophagyAutophagic pathwayHost DNADendritic cellsAutoimmune disordersReceptor 9Interferon secretionChain 3Immune complexesProtein 3DNAInterferon
2010
In Vivo Requirement for Atg5 in Antigen Presentation by Dendritic Cells
Lee HK, Mattei LM, Steinberg BE, Alberts P, Lee YH, Chervonsky A, Mizushima N, Grinstein S, Iwasaki A. In Vivo Requirement for Atg5 in Antigen Presentation by Dendritic Cells. Immunity 2010, 32: 227-239. PMID: 20171125, PMCID: PMC2996467, DOI: 10.1016/j.immuni.2009.12.006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationAutophagy-Related Protein 5Cells, CulturedDendritic CellsFemaleHerpes SimplexHerpesvirus 2, HumanHistocompatibility Antigens Class IILymphocyte ActivationMiceMice, Inbred C57BLMice, KnockoutMicrotubule-Associated ProteinsRadiation ChimeraRNA, Small InterferingConceptsMHC-II presentationMHC class IIDendritic cellsAntigen presentationClass IIHerpes simplex virus infectionToll-like receptor stimuliT cell primingSimplex virus infectionCell primingAbsence of ATG5Microbial antigensVirus infectionMHC ICytosolic antigensConditional deletionAntigenReceptor stimuliAutophagic machineryKey autophagy genesRapid diseasePresentationATG5Lysosome fusionAutophagy genes
2009
Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling
Tal MC, Sasai M, Lee HK, Yordy B, Shadel GS, Iwasaki A. Absence of autophagy results in reactive oxygen species-dependent amplification of RLR signaling. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 2770-2775. PMID: 19196953, PMCID: PMC2650341, DOI: 10.1073/pnas.0807694106.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutophagyAutophagy-Related Protein 5Cells, CulturedDEAD Box Protein 58DEAD-box RNA HelicasesDNA, MitochondrialEnzyme-Linked Immunosorbent AssayFlow CytometryInterferon Type IMacrophagesMiceMicrotubule-Associated ProteinsMitochondriaReactive Oxygen SpeciesReverse Transcriptase Polymerase Chain ReactionSignal TransductionConceptsReactive oxygen speciesDysfunctional mitochondriaInnate antiviral defenseAntiviral defenseKey antiviral cytokinesAbsence of autophagyMitochondrial reactive oxygen speciesHomeostatic regulationRole of autophagyTreatment of cellsIPS-1RLR signalingVesicular stomatitis virusAutophagy resultsRNA virusesWT cellsMitochondriaAutophagyType I IFNStomatitis virusRLRLike receptorsOxygen speciesNeurodegenerative diseasesInflammatory disorders
2008
The autophagy gene ATG5 plays an essential role in B lymphocyte development
Miller BC, Zhao Z, Stephenson LM, Cadwell K, Pua HH, Lee HK, Mizushima NN, Iwasaki A, He YW, Swat W, Virgin HW. The autophagy gene ATG5 plays an essential role in B lymphocyte development. Autophagy 2008, 4: 309-314. PMID: 18188005, DOI: 10.4161/auto.5474.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutophagyAutophagy-Related Protein 5B-LymphocytesBone Marrow CellsCell DeathCell LineageCell SurvivalMiceMice, KnockoutMicrotubule-Associated ProteinsPeritoneal CavityConceptsB cell developmentCell developmentGene ATG5Pre-B cell transitionB lymphocyte developmentDeletion of Atg5Fetal liver progenitorsAutophagy gene ATG5B cell survivalCell lineagesCell transitionLymphocyte developmentCell survivalB-1a B cellsCell deathCre-loxP technologyCytoplasmic constituentsATG5Liver progenitorsEssential roleSignificant defectsB cellsDiscrete stagesCell numberCells