2023
PD-1highCXCR5–CD4+ peripheral helper T cells promote CXCR3+ plasmablasts in human acute viral infection
Asashima H, Mohanty S, Comi M, Ruff W, Hoehn K, Wong P, Klein J, Lucas C, Cohen I, Coffey S, Lele N, Greta L, Raddassi K, Chaudhary O, Unterman A, Emu B, Kleinstein S, Montgomery R, Iwasaki A, Dela Cruz C, Kaminski N, Shaw A, Hafler D, Sumida T. PD-1highCXCR5–CD4+ peripheral helper T cells promote CXCR3+ plasmablasts in human acute viral infection. Cell Reports 2023, 42: 111895. PMID: 36596303, PMCID: PMC9806868, DOI: 10.1016/j.celrep.2022.111895.Peer-Reviewed Original ResearchConceptsAcute viral infectionTph cellsViral infectionCXCR3 expressionClinical outcomesHelper TSevere viral infectionsB cell helpBetter clinical outcomesProtective humoral immunityT cell-B cell interactionsKey immune responsesPlasmablast expansionB cell differentiationCell subsetsHumoral immunityCell helpImmune responseInterferon γPlasmablast differentiationB cellsPlasmablastsCell responsesInfectionCD4
2021
B cells join T cell clusters in the host response to recurrent herpes simplex virus 2 infection
Gehlhausen JR, Iwasaki A. B cells join T cell clusters in the host response to recurrent herpes simplex virus 2 infection. Journal Of Clinical Investigation 2021, 131: e148300. PMID: 33938452, PMCID: PMC8087191, DOI: 10.1172/jci148300.Peer-Reviewed Original ResearchConceptsAntibody-secreting cellsB cellsRecurrent lesionsHerpes simplex virus 2 infectionHSV-2-specific antibodiesRecurrent genital herpes lesionsSimplex virus 2 infectionGenital herpes lesionsHSV-2 lesionsVirus 2 infectionT cell clustersSkin biopsy specimensB cell subsetsB cell populationsNaive B cellsStage of infectionLesion reactivationDisease resolutionImmune infiltratesAntibody levelsHerpes lesionsCell subsetsBiopsy specimensHost responseLesions
2015
Toll-like receptor 9 trafficking and signaling for type I interferons requires PIKfyve activity
Hayashi K, Sasai M, Iwasaki A. Toll-like receptor 9 trafficking and signaling for type I interferons requires PIKfyve activity. International Immunology 2015, 27: 435-445. PMID: 25925170, PMCID: PMC4560039, DOI: 10.1093/intimm/dxv021.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell MembraneDendritic CellsDNA-Binding ProteinsEndosomesInterferon Type ILysosome-Associated Membrane GlycoproteinsMiceMice, Inbred C57BLPhosphatidylinositol 3-KinasesProtein TransportSignal TransductionToll-Like Receptor 7Toll-Like Receptor 9Transcription FactorsVesicle-Associated Membrane Protein 3ConceptsDifferent dendritic cell subsetsIFN inductionDendritic cell subsetsInduction of cytokinesType I IFN inductionType I IFNType I interferonI IFN inductionViral nucleic acidsAnti-viral programsTLR9 traffickingCell subsetsTLR9 signalsI IFNI interferonInhibitor treatmentDistinct subcellular membranesRAW264.7 cellsType I interferon (IFN) genesTLR9Distinct endosomal compartmentsInterferon genesTLR traffickingConfocal microscopyInduction
2000
Localization of Distinct Peyer's Patch Dendritic Cell Subsets and Their Recruitment by Chemokines Macrophage Inflammatory Protein (Mip)-3α, Mip-3β, and Secondary Lymphoid Organ Chemokine
Iwasaki A, Kelsall B. Localization of Distinct Peyer's Patch Dendritic Cell Subsets and Their Recruitment by Chemokines Macrophage Inflammatory Protein (Mip)-3α, Mip-3β, and Secondary Lymphoid Organ Chemokine. Journal Of Experimental Medicine 2000, 191: 1381-1394. PMID: 10770804, PMCID: PMC2193144, DOI: 10.1084/jem.191.8.1381.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceChemokine CCL19Chemokine CCL20Chemokines, CCChemotaxisDendritic CellsDNA PrimersFemaleGene ExpressionIn Situ HybridizationMacrophage Inflammatory ProteinsMiceMice, Inbred BALB CMicroscopy, ConfocalModels, BiologicalPeyer's PatchesReceptors, CCR6Receptors, CCR7Receptors, ChemokineRNA, MessengerSpleenConceptsDendritic cell subsetsInterfollicular regionsDC subsetsCell subsetsInflammatory proteinPeyer's patchesSecondary lymphoid organ chemokineChemokine macrophage inflammatory proteinSplenic DC subsetsSubepithelial dome regionDistinct dendritic cell subsetsRole of chemokinesT cell responsesMacrophage inflammatory proteinT-cell regionsFollicle-associated epitheliumMurine Peyer's patchesTranscriptase-polymerase chain reaction analysisFunctional CCR7Lymphoid DCsMIP-3βPP DCsMyeloid DCsCCR7 expressionPolymerase chain reaction analysis