Methylation

Infinium MethylationEPIC Kit

With the Infinium MethylationEPIC BeadChip Kit, researchers can interrogate over 850,000 methylation sites quantitatively across the genome at single-nucleotide resolution. Multiple samples, including FFPE, can be analyzed in parallel to deliver high-throughput power while minimizing the cost per sample.

Infinium HD array technology enables content selection independent of bias-associated limitations often associated with methylated DNA capture methods. The result is a pan-enhancer and coding region view of the methylome that can be used for epigenome-wide association studies on a variety of human tissues.

Comprehensive Genome-Wide Coverage

This kit includes the following content categories requested by methylation experts:

  • CpG sites outside of CpG islands
  • Non-CpG methylated sites identified in human stem cells (CHH sites)
  • Differentially methylated sites identified in tumor versus normal
  • FANTOM5 enhancers
  • ENCODE open chromatin and enhancers
  • DNase hypersensitive sites
  • miRNA promoter regions
  • > 90% of content contained on the Illumina HumanMethylation450K BeadChip
Integrated Analysis Software

MethylationEPIC data analysis is supported by the GenomeStudio Methylation Module. GenomeStudio software enables researchers to effortlessly perform differential methylation analysis for small-scale studies. It features advanced visualization tools that enable researchers to view vast amounts of data in a single graph, such as heat maps, scatter plots, and line plots.

These tools and the GenomeStudio Genome Browser display valuable information such as chromosomal coordinates, percent GC, location in a CpG Island, and methylation β values.

For large-scale studies, there are many free analysis packages that work in the software framework R for normalization, and differential analysis of methylation data. Instructional videos explain the use of these data analysis packages.