2018
Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis
Zhao H, Roistacher D, Helmann J. Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis. Molecular Microbiology 2018, 109: 826-844. PMID: 29995990, PMCID: PMC6185803, DOI: 10.1111/mmi.14078.Peer-Reviewed Original ResearchConceptsDifco sporulation mediumPeptidoglycan synthesisCentral metabolismCell envelope stress responseEnvelope stress responseCell wall synthesisCell lysisWall synthesisSensitizes cellsStress responseAlteration of metabolismUptake systemBacillus subtilisLater stepsAmino acid analysisDiaminopimelateLB mediumSporulation mediumAcid analysisPhysiological studiesSynthesis inhibitorCompetitive inhibitorMetabolismImportant targetCellsRNA tales – how embryos read and discard messages from mom
Despic V, Neugebauer KM. RNA tales – how embryos read and discard messages from mom. Journal Of Cell Science 2018, 131: jcs201996. PMID: 29467249, DOI: 10.1242/jcs.201996.Peer-Reviewed Original ResearchConceptsZygotic genomePost-transcriptional regulatory pathwaysMaternal mRNA clearanceMaternal mRNA degradationEarly developmentContribution of microRNAsZygotic transitionMRNA clearanceRNA regulationMRNA decayCellular contextHigh-throughput methodZebrafish embryosFemale gametesMaternal mRNAsMolecular playersMRNA degradationRNA modificationsMolecular principlesRegulatory pathwaysGenomeLater stepsMZTSilent genomeEmbryos
2012
Genetics of germ cell development
Lesch BJ, Page DC. Genetics of germ cell development. Nature Reviews Genetics 2012, 13: 781-794. PMID: 23044825, DOI: 10.1038/nrg3294.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsGerm cell developmentGerm cellsHaploid genomeMultiple speciesCell developmentPluripotent stem cell systemsRepressive chromatin configurationGerm cell identityRNA polymerase IIGerm cell biologyStem cell systemEarly germ cellsPolymerase IICell identityDiploid genomeChromatin configurationIndividual organismsCell biologyMitotic proliferationGenomeSame speciesDifferent speciesGermline precursorsLater stepsMeiosis
2009
Axon Regeneration Requires a Conserved MAP Kinase Pathway
Hammarlund M, Nix P, Hauth L, Jorgensen EM, Bastiani M. Axon Regeneration Requires a Conserved MAP Kinase Pathway. Science 2009, 323: 802-806. PMID: 19164707, PMCID: PMC2729122, DOI: 10.1126/science.1165527.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAxonsAxotomyCaenorhabditis elegansCaenorhabditis elegans Proteinsgamma-Aminobutyric AcidGrowth ConesMAP Kinase Kinase 4MAP Kinase Kinase KinasesMAP Kinase Signaling SystemMitogen-Activated Protein KinasesModels, BiologicalMotor NeuronsMutationNerve RegenerationRNA InterferenceConceptsCell-intrinsic regeneration pathwaysKinase pathwayCaenorhabditis elegans motor neuronsMitogen-activated protein kinase pathwayMAP kinase cascadeMAP kinase pathwayProtein kinase pathwayGrowth cone migrationKinase cascadeRegeneration pathwayCone migrationLater stepsLack of activationDevelopment of therapiesMature neuronsPathwayAplastic statesMost neuronsRegenerationMotor neuronsAxon injuryAxon regenerationNeuron functionActivationNeurons
2005
MicroRNAs Regulate Brain Morphogenesis in Zebrafish
Giraldez AJ, Cinalli RM, Glasner ME, Enright AJ, Thomson JM, Baskerville S, Hammond SM, Bartel DP, Schier AF. MicroRNAs Regulate Brain Morphogenesis in Zebrafish. Science 2005, 308: 833-838. PMID: 15774722, DOI: 10.1126/science.1109020.Peer-Reviewed Original ResearchConceptsRNA-binding domainMultiple cell typesDicer mutantsMutant embryosSmall RNAsPrecursor miRNAsAxis formationMature miRNAsRibonuclease IIIHeart developmentGene expressionBrain morphogenesisAbnormal morphogenesisLater stepsMiRNA formationMorphogenesisMutantsMiRNAsCell typesBrain formationEssential roleZebrafishBrain defectsSomitogenesisGastrulation
2000
Microvillus Inclusion Disease: A Genetic Defect Affecting Apical Membrane Protein Traffic in Intestinal Epithelium
Ameen N, Salas P. Microvillus Inclusion Disease: A Genetic Defect Affecting Apical Membrane Protein Traffic in Intestinal Epithelium. Traffic 2000, 1: 76-83. PMID: 11208062, DOI: 10.1034/j.1600-0854.2000.010111.x.Peer-Reviewed Original ResearchConceptsMicrovillus inclusion diseaseApical membrane trafficMembrane protein trafficVacuolar apical compartmentApical membrane markersMicrovillus inclusionsF-actin layerBasolateral proteinsFirst genetic defectMembrane trafficTissue culture epithelial cellsProtein trafficApical exocytosisLater stepsApical membraneMembrane markersTerminal webImmunofluorescence analysisApical cytoplasmGenetic defectsEpithelial cellsProteinApical compartmentIntestinal epitheliumNormal microtubules
1998
Critical Amino Acid Residues in Transmembrane Span 7 of the Serotonin Transporter Identified by Random Mutagenesis*
Penado K, Rudnick G, Stephan M. Critical Amino Acid Residues in Transmembrane Span 7 of the Serotonin Transporter Identified by Random Mutagenesis*. Journal Of Biological Chemistry 1998, 273: 28098-28106. PMID: 9774428, DOI: 10.1074/jbc.273.43.28098.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBiological TransportCarrier ProteinsMembrane GlycoproteinsMembrane Transport ProteinsModels, MolecularMolecular Sequence DataMutagenesisNerve Tissue ProteinsProtein ConformationRatsSerotoninSerotonin Plasma Membrane Transport ProteinsStructure-Activity RelationshipConceptsAmino acid residuesRandom mutagenesisAcid residuesTransport activityCritical amino acid residuesRat brain serotonin transporterCritical residuesTransport cycleWild typeNonconservative mutationsStructural predictionsTransporter functionLater stepsMutationsSerotonin transporterResiduesMutagenesisHydrophobic substitutionsTyr-385TransportersMutantsActivitySubstitutionNearby positions
1988
A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells.
Steitz JA, Berg C, Hendrick JP, La Branche-Chabot H, Metspalu A, Rinke J, Yario T. A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells. Journal Of Cell Biology 1988, 106: 545-556. PMID: 3279045, PMCID: PMC2115095, DOI: 10.1083/jcb.106.3.545.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoantibodiesCell NucleolusCentrifugation, Density GradientElectrophoresis, Polyacrylamide GelFluorescent Antibody TechniqueFriend murine leukemia virusHeLa CellsHumansImmunoassayLeukemia, Erythroblastic, AcuteRibonucleoproteinsRibosomal ProteinsRibosomesRNA PrecursorsRNA, RibosomalRNA, Ribosomal, 5STumor Cells, CulturedConceptsRNA-protein complexesVivo pulse-chase experimentsRibosomal protein L5Pulse-chase experimentsProtein complexesProtein L5Mammalian cellsRNA moleculesMouse cellsLater stepsHeLa cellsProtein moietyRNPRibosomesNucleoliCellsComplexesAssemblyBiogenesisRRNAIndirect immunofluorescenceSubunitsRNAImmunofluorescence
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply