2018
Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis
Zhao H, Roistacher D, Helmann J. Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis. Molecular Microbiology 2018, 109: 826-844. PMID: 29995990, PMCID: PMC6185803, DOI: 10.1111/mmi.14078.Peer-Reviewed Original ResearchConceptsDifco sporulation mediumPeptidoglycan synthesisCentral metabolismCell envelope stress responseEnvelope stress responseCell wall synthesisCell lysisWall synthesisSensitizes cellsStress responseAlteration of metabolismUptake systemBacillus subtilisLater stepsAmino acid analysisDiaminopimelateLB mediumSporulation mediumAcid analysisPhysiological studiesSynthesis inhibitorCompetitive inhibitorMetabolismImportant targetCellsThe Stringent Response Determines the Ability of a Commensal Bacterium to Survive Starvation and to Persist in the Gut
Schofield WB, Zimmermann-Kogadeeva M, Zimmermann M, Barry NA, Goodman AL. The Stringent Response Determines the Ability of a Commensal Bacterium to Survive Starvation and to Persist in the Gut. Cell Host & Microbe 2018, 24: 120-132.e6. PMID: 30008292, PMCID: PMC6086485, DOI: 10.1016/j.chom.2018.06.002.Peer-Reviewed Original ResearchConceptsCarbon starvationStringent responseHuman gut bacterium Bacteroides thetaiotaomicronTricarboxylic acid cycle genesMultiple biosynthetic pathwaysCycle genesCentral metabolismMammalian gutTriggers accumulationBiosynthetic pathwayBacteroides thetaiotaomicronDeficient strainMetabolic regulatorAlpha-ketoglutarate supplementationStarvationAlpha-ketoglutarateC labelingCommensal bacteriaMetabolomic analysisGut microbiomeCommensal bacteriumThetaiotaomicronBacteriaPathwayGut
2016
Multiple Signals Govern Utilization of a Polysaccharide in the Gut Bacterium Bacteroides thetaiotaomicron
Schwalm ND, Townsend GE, Groisman EA. Multiple Signals Govern Utilization of a Polysaccharide in the Gut Bacterium Bacteroides thetaiotaomicron. MBio 2016, 7: 10.1128/mbio.01342-16. PMID: 27729509, PMCID: PMC5061871, DOI: 10.1128/mbio.01342-16.Peer-Reviewed Original ResearchConceptsMammalian gut microbiotaPolysaccharide utilization genesUtilization genesHost dietGut symbiont Bacteroides thetaiotaomicronComplex polysaccharidesBacteroides thetaiotaomicronPleiotropic transcriptional regulatorDomains of lifeSubset of organismsRegulatory paradigmCritical nutrient sourceTranscriptional activatorTranscriptional regulatorsTranscriptional repressorCentral metabolismGenomic analysisMammalian gutMammalian hostsRegulatory architectureGut BacteroidesPolymeric fructanGut microbiotaGut bacteriumPolysaccharide breakdown
2014
A Screen of Coxiella burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis
Newton HJ, Kohler LJ, McDonough JA, Temoche-Diaz M, Crabill E, Hartland EL, Roy CR. A Screen of Coxiella burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis. PLOS Pathogens 2014, 10: e1004286. PMID: 25080348, PMCID: PMC4117601, DOI: 10.1371/journal.ppat.1004286.Peer-Reviewed Original ResearchConceptsHost autophagyTransposon insertionDot/Icm effectorsDot/Icm systemAutophagosome protein LC3Intracellular growth defectPathogen-occupied vacuolesTransposon insertion mutantsVacuole biogenesisEffector proteinsInsertion mutantsModification enzymesGrowth defectArrayed libraryCentral metabolismIcm systemMutantsMolecular mechanismsVisual screenProtein LC3Host cellsIntracellular replicationGenesIntracellular pathogensRegulatory system
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