2018
Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis
Zhao H, Roistacher D, Helmann J. Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis. Molecular Microbiology 2018, 109: 826-844. PMID: 29995990, PMCID: PMC6185803, DOI: 10.1111/mmi.14078.Peer-Reviewed Original ResearchConceptsDifco sporulation mediumPeptidoglycan synthesisCentral metabolismCell envelope stress responseEnvelope stress responseCell wall synthesisCell lysisWall synthesisSensitizes cellsStress responseAlteration of metabolismUptake systemBacillus subtilisLater stepsAmino acid analysisDiaminopimelateLB mediumSporulation mediumAcid analysisPhysiological studiesSynthesis inhibitorCompetitive inhibitorMetabolismImportant targetCellsMultisystem Analysis of Mycobacterium tuberculosis Reveals Kinase-Dependent Remodeling of the Pathogen-Environment Interface
Carette X, Platig J, Young D, Helmel M, Young A, Wang Z, Potluri L, Moody C, Zeng J, Prisic S, Paulson J, Muntel J, Madduri A, Velarde J, Mayfield J, Locher C, Wang T, Quackenbush J, Rhee K, Moody D, Steen H, Husson R. Multisystem Analysis of Mycobacterium tuberculosis Reveals Kinase-Dependent Remodeling of the Pathogen-Environment Interface. MBio 2018, 9: 10.1128/mbio.02333-17. PMID: 29511081, PMCID: PMC5845002, DOI: 10.1128/mbio.02333-17.Peer-Reviewed Original ResearchConceptsMycobacterial cell envelopeCell envelopePeptidoglycan turnoverProtein abundanceResponse regulator MtrAProtein secretion systemStress responseEnvelope stress responsePhosphorylated cellular proteinsRegulate essential processesDifferentially phosphorylated proteinsSerine/threonine protein kinaseTransport of proteinsLevel of gene expressionAnalysis of phosphoproteinsEssential cell functionsLevel of phosphorylationDNA binding activityDecreased expression of genesCell wall lipidsExpression of genesLipid contentIncreasing multidrug resistanceSecretion systemVirulence determinants
2016
Depletion of Undecaprenyl Pyrophosphate Phosphatases Disrupts Cell Envelope Biogenesis in Bacillus subtilis
Zhao H, Sun Y, Peters J, Gross C, Garner E, Helmann J. Depletion of Undecaprenyl Pyrophosphate Phosphatases Disrupts Cell Envelope Biogenesis in Bacillus subtilis. Journal Of Bacteriology 2016, 198: 2925-2935. PMID: 27528508, PMCID: PMC5055597, DOI: 10.1128/jb.00507-16.Peer-Reviewed Original ResearchConceptsUndecaprenyl pyrophosphateCRISPRi systemCell envelope stress responseSynthetic lethal gene pairsTranscriptional repression systemWall teichoic acid synthesisBacillus subtilisEnvelope stress responseCell envelope biogenesisCell envelope synthesisSet of genesLipid II cycleLipid-linked precursorsShort palindromic repeats (CRISPR) systemTeichoic acid synthesisPalindromic repeats systemWall teichoic acidBacterial cell envelopeHigh turgor pressureGenetic redundancyEnvelope biogenesisEssential genesLipid phosphataseRepression systemFunctional redundancy
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