2025
Mutant prion protein enhances NMDA receptor activity, activates PKC, and triggers rapid excitotoxicity in mice
Lin J, Callender J, Mayfield J, McClatchy D, Ojeda-Juárez D, Pourhamzeh M, Soldau K, Kurt T, Danque G, Khuu H, Ronson J, Pizzo D, Du Y, Gruber M, Sevillano A, Wang J, Orrú C, Chen J, Funk G, Aguilar-Calvo P, Aulston B, Roy S, Rho J, Bui J, Newton A, Lipton S, Caughey B, Patrick G, Doré K, Yates J, Sigurdson C. Mutant prion protein enhances NMDA receptor activity, activates PKC, and triggers rapid excitotoxicity in mice. Journal Of Clinical Investigation 2025, 135: e186432. PMID: 40185484, PMCID: PMC12077891, DOI: 10.1172/jci186432.Peer-Reviewed Original ResearchConceptsN-methyl-D-aspartate receptorsProtein kinase CAmino terminusPrion proteinN-methyl-D-aspartateMutant prion proteinNMDA receptor activationN-linked glycosylation sitesExcitatory-inhibitory imbalanceHippocampal pyramidal neuronsDownstream signaling eventsActivate protein kinase CNMDAR channelsNeuronal hyperexcitabilityFunctional motifsGlutamate receptorsCalcium influxPhosphoproteomic analysisPyramidal neuronsGlycosylation sitesSignaling eventsReceptor activationPrion-infected miceDendritic beadingSynapse loss
2023
KIF4 Gene Variant’s Disruption of PARP1 Signaling Increases Anxiety and Seizure Susceptibility
Bordey A. KIF4 Gene Variant’s Disruption of PARP1 Signaling Increases Anxiety and Seizure Susceptibility. Epilepsy Currents 2023, 23: 257-258. PMID: 37662464, PMCID: PMC10470092, DOI: 10.1177/15357597231175007.Peer-Reviewed Original ResearchSeizure susceptibilityDevelopmental delayLower seizure thresholdHippocampal pyramidal neuronsSeizure susceptibility phenotypesCommon neurological diseasesPoly (ADP-ribose) polymeraseMild developmental delayKCC2 pathwaySeizure thresholdPyramidal neuronsEpilepsy treatmentMember of kinesinNeurological diseasesNeurological disordersBehavioral testsNeuronal morphologyMutant miceGenetic abnormalitiesIncreases anxietyPotential targetIntellectual disabilityEpilepsySignaling pathwaysSusceptibility phenotype
2021
Selective Overexpression of Collybistin in Mouse Hippocampal Pyramidal Cells Enhances GABAergic Neurotransmission and Protects against PTZ-Induced Seizures
George S, James S, De Blas A. Selective Overexpression of Collybistin in Mouse Hippocampal Pyramidal Cells Enhances GABAergic Neurotransmission and Protects against PTZ-Induced Seizures. ENeuro 2021, 8: eneuro.0561-20.2021. PMID: 34083383, PMCID: PMC8281261, DOI: 10.1523/eneuro.0561-20.2021.Peer-Reviewed Original ResearchConceptsHippocampal pyramidal cellsPyramidal cellsPyramidal neuronsAdeno-associated virusGABAergic synaptic transmissionCortical pyramidal neuronsHippocampal pyramidal neuronsMouse hippocampal pyramidal cellsCultured hippocampal neuronsAbsence of gephyrinCre-dependent mannerGABAergic neurotransmissionCre miceInhibitory neurotransmissionHippocampal interneuronsInhibitory postsynapsesInhibitory synapsesHippocampal neuronsSynaptic transmissionTarget neuronsPostsynaptic sitesBrain regionsMice resultsSelective overexpressionGlycinergic postsynapses
2019
Prokaryotic SPHINX replication sequences are conserved in mammalian brain and participate in neurodegeneration
Szigeti‐Buck K, Manuelidis L. Prokaryotic SPHINX replication sequences are conserved in mammalian brain and participate in neurodegeneration. Journal Of Cellular Biochemistry 2019, 120: 17687-17698. PMID: 31231867, DOI: 10.1002/jcb.29035.Peer-Reviewed Original ResearchConceptsCreutzfeldt-Jakob diseaseGuinea pigsMammalian brainSporadic Creutzfeldt-Jakob diseaseOnly excitatory neuronsHippocampal pyramidal neuronsGranule cell layerInternal granule cell layerPancreatic islet cellsPyramidal neuronsSporadic CJDHidden infectionType synapsesExcitatory synapsesExcitatory neuronsMossy fibersPurkinje neuronsProgressive neurodegenerationNeuron synapsesIslet cellsSpecific neuronsWestern blotNeuronsPancreatic exocrine cellsKidney tubules
2016
Biophysical Factors that Promote Mechanically-Induced Action Potentials in Neocortical and Hippocampal Pyramidal Neurons
Nikolaev Y, Dosen P, Laver D, Van Helden D, Hamill O. Biophysical Factors that Promote Mechanically-Induced Action Potentials in Neocortical and Hippocampal Pyramidal Neurons. Biophysical Journal 2016, 110: 349a. DOI: 10.1016/j.bpj.2015.11.1877.Peer-Reviewed Original Research
2015
Single mechanically-gated cation channel currents can trigger action potentials in neocortical and hippocampal pyramidal neurons
Nikolaev Y, Dosen P, Laver D, van Helden D, Hamill O. Single mechanically-gated cation channel currents can trigger action potentials in neocortical and hippocampal pyramidal neurons. Brain Research 2015, 1608: 1-13. PMID: 25765154, DOI: 10.1016/j.brainres.2015.02.051.Peer-Reviewed Original ResearchConceptsHippocampal pyramidal neuronsPyramidal neuronsChannel currentsSpike activityAction potentialsRhythmic spike activityCell-attached patch recordingsTraumatic brain injuryCerebellar Purkinje neuronsMouse brain slicesCation channel currentsCentral neuronsBrain injuryLocus coeruleusBrain slicesSynaptic inputsVoltage-gated channelsPurkinje neuronsPatch recordingsInward currentsMammalian brainNeuron typesNeuronsMechanosensitive organCation channels
2011
Neuroprotective effects of progesterone and allopregnanolone on long-term cognitive outcome after global cerebral ischemia
Moralí G, Montes P, Hernández-Morales L, Monfil T, Espinosa-García C, Cervantes M. Neuroprotective effects of progesterone and allopregnanolone on long-term cognitive outcome after global cerebral ischemia. Restorative Neurology And Neuroscience 2011, 29: 1-15. PMID: 21335664, DOI: 10.3233/rnn-2011-0571.Peer-Reviewed Original ResearchConceptsNeuroprotective effects of progesteroneEffects of progesteroneTreated ratsGlobal cerebral ischemiaLoss of hippocampal pyramidal neuronsPyramidal neuronsLong-term cognitive outcomesCerebral ischemiaAdult male Sprague-Dawley ratsMale Sprague-Dawley ratsLoss of pyramidal neuronsHippocampal pyramidal neuronsSprague-Dawley ratsWorking memoryMemory testTreated with P4Spatial learningCognitive outcomesAllopregnanoloneNeural conditionsIntact ratsHippocampusSham procedureForebrain ischemiaNonischemic controls
2010
Imaging Inhibitory Synaptic Potentials Using Voltage Sensitive Dyes
Canepari M, Willadt S, Zecevic D, Vogt KE. Imaging Inhibitory Synaptic Potentials Using Voltage Sensitive Dyes. Biophysical Journal 2010, 98: 2032-2040. PMID: 20441768, PMCID: PMC2862202, DOI: 10.1016/j.bpj.2010.01.024.Peer-Reviewed Original ResearchConceptsInhibitory postsynaptic potentialsVoltage-sensitive dyeGABAergic inhibitory postsynaptic potentialsCA1 hippocampal pyramidal neuronsPatch electrodeInhibitory synaptic potentialsInhibitory synaptic transmissionHippocampal pyramidal neuronsIntracellular chloride concentrationWhole-cell configurationPyramidal neuronsApical dendritesPostsynaptic potentialsSynaptic potentialsSynaptic transmissionBrain slicesElectrode recordingsNeuronsSensitive dyeVoltage imaging techniqueDendritesMultiple sitesVoltage imagingAxons
2004
Fibroblast Growth Factor Receptor 1 Is Required for the Proliferation of Hippocampal Progenitor Cells and for Hippocampal Growth in Mouse
Ohkubo Y, Uchida AO, Shin D, Partanen J, Vaccarino FM. Fibroblast Growth Factor Receptor 1 Is Required for the Proliferation of Hippocampal Progenitor Cells and for Hippocampal Growth in Mouse. Journal Of Neuroscience 2004, 24: 6057-6069. PMID: 15240797, PMCID: PMC6729672, DOI: 10.1523/jneurosci.1140-04.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, DifferentiationCell CountCell LineageCell ProliferationCells, CulturedHeredodegenerative Disorders, Nervous SystemHippocampusHumansIn Situ HybridizationLateral VentriclesMiceMice, TransgenicMutagenesis, Site-DirectedNeurogliaNeuronsPyramidal CellsReceptor Protein-Tyrosine KinasesReceptor, Fibroblast Growth Factor, Type 1Receptors, Fibroblast Growth FactorRNA, MessengerStem CellsTransgenesConceptsHippocampal ventricular zonesDentate gyrusGrowth factor receptor 1Fibroblast growth factor receptor 1Factor receptor 1Ventricular zoneNeural stem cellsPyramidal neuronsHippocampal growthProgenitor cellsGranule cellsReceptor 1Glial fibrillary acidic protein promoterHuman glial fibrillary acidic protein promoterEmbryonic dorsal telencephalonRadial glial-like cellsRadial glial progenitor cellsHippocampal dentate gyrusParvalbumin-containing interneuronsDG granule cellsHippocampal pyramidal neuronsStem cellsHippocampal progenitor cellsRole of FGFR1Glial progenitor cells
2003
Electric field modulation of synchronization in neuronal networks
Park E, So P, Barreto E, Gluckman B, Schiff S. Electric field modulation of synchronization in neuronal networks. Neurocomputing 2003, 52: 169-175. DOI: 10.1016/s0925-2312(02)00820-2.Peer-Reviewed Original Research
1999
Increased neurodegeneration during ageing in mice lacking high‐affinity nicotine receptors
Zoli M, Picciotto M, Ferrari R, Cocchi D, Changeux J. Increased neurodegeneration during ageing in mice lacking high‐affinity nicotine receptors. The EMBO Journal 1999, 18: 1235-1244. PMID: 10064590, PMCID: PMC1171214, DOI: 10.1093/emboj/18.5.1235.Peer-Reviewed Original ResearchConceptsHigh-affinity nicotine receptorsSpatial learningHippocampal pyramidal neuronsRegion-specific alterationsSerum corticosterone levelsPossible animal modelNicotinic acetylcholine receptorsPyramidal neuronsNeuronal survivalNicotine receptorsCholinergic systemEndocrine parametersControl animalsIncreased neurodegenerationAnimal modelsCorticosterone levelsAcetylcholine receptorsAlzheimer's diseaseDegenerative processCortical regionsMorris mazeCognitive deficitsMutant miceMiceBeta2 subunit
1993
Ischemia as an Excitotoxic Lesion: Protection Against Hippocampal Nerve Cell Loss by Denervation
Diemer N, Johansen F, Benveniste H, Bruhn T, Berg M, Valente E, Jørgensen M. Ischemia as an Excitotoxic Lesion: Protection Against Hippocampal Nerve Cell Loss by Denervation. Acta Neurochirurgica Supplement 1993, 57: 94-101. PMID: 8380675, DOI: 10.1007/978-3-7091-9266-5_14.Peer-Reviewed Original ResearchConceptsPyramidal neuronsIschemic neuron damageVulnerable nerve cellsTransient cerebral ischemiaGABA-ergic interneuronsNerve cell lossMin of ischemiaHippocampal pyramidal neuronsRelease of glutamateExtracellular glutamate releaseKainate/Neuroexcitatory mechanismsNeuron damagePostischemic periodCerebral ischemiaHilus cellsPostischemic hypoperfusionCalcium blockersGlutamate releaseIntact innervationIschemic animalsDenervated ratsDentate hilusReceptor autoradiographyCA1 neurons
1992
A model of NMDA receptor-mediated activity in dendrites of hippocampal CA1 pyramidal neurons
Pongracz F, Poolos N, Kocsis J, Shepherd G. A model of NMDA receptor-mediated activity in dendrites of hippocampal CA1 pyramidal neurons. Journal Of Neurophysiology 1992, 68: 2248-2259. PMID: 1337105, PMCID: PMC2605954, DOI: 10.1152/jn.1992.68.6.2248.Peer-Reviewed Original ResearchConceptsReceptor-mediated conductanceExcitatory postsynaptic potentialsPyramidal neuronsSynaptic responsesSynaptic activationSize of EPSPsExtracellular ion concentration changesNMDA receptor-mediated activityCA1 hippocampal pyramidal cellsCA1 hippocampal pyramidal neuronsHippocampal CA1 pyramidal neuronsDendritic NMDA receptorsRepetitive synaptic activationCA1 pyramidal neuronsInhibitory synaptic responsesFrequency-dependent depressionHippocampal pyramidal neuronsNMDA receptor activationHippocampal pyramidal cellsExtracellular potassium accumulationRepetitive activityIntrinsic membrane conductancesReceptor-mediated activityHz. 4Spike afterdepolarization
1991
Enhancement of the Glutamate Response by cAMP-Dependent Protein Kinase in Hippocampal Neurons
Greengard P, Jen J, Nairn A, Stevens C. Enhancement of the Glutamate Response by cAMP-Dependent Protein Kinase in Hippocampal Neurons. Science 1991, 253: 1135-1138. PMID: 1716001, DOI: 10.1126/science.1716001.Peer-Reviewed Original ResearchConceptsProtein kinaseCAMP-dependent protein kinaseGlutamate receptor channelsMonophosphate-dependent protein kinaseReceptor channelsType glutamate receptor channelsAdenylate cyclase cascadeCultured hippocampal pyramidal neuronsSpontaneous excitatory postsynaptic currentsWhole-cell current responsesSingle-channel analysisNeuromodulatory regulationMammalian brainExcitatory postsynaptic currentsHippocampal pyramidal neuronsKinaseLong-term potentiationPyramidal neuronsPostsynaptic currentsGlutamate responseExcitatory neurotransmitterMean open timeHippocampal neuronsAdenylate cyclaseSynaptic events
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