2011
Two types of chloride transporters are required for GABAA receptor‐mediated inhibition in C. elegans
Bellemer A, Hirata T, Romero MF, Koelle MR. Two types of chloride transporters are required for GABAA receptor‐mediated inhibition in C. elegans. The EMBO Journal 2011, 30: 1852-1863. PMID: 21427702, PMCID: PMC3101993, DOI: 10.1038/emboj.2011.83.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedAnion Transport ProteinsBrainCaenorhabditis elegansCaenorhabditis elegans ProteinsChloridesElectrophysiologyGene Expression RegulationHydrogen-Ion ConcentrationMicroscopyMotor ActivityMutationNeuronsOocytesPlasmidsReceptors, GABA-ASymportersTransgenesXenopusConceptsCaenorhabditis elegans mutantC. elegansSynapse developmentInhibits cellBehavioral defectsCl- gradientGABAA receptor-mediated inhibitionMutantsReceptor-mediated inhibitionTransportersChloride transportersCl- channelsIdentified mutationsNeuronal expressionCl(-) cotransporterCl(-) extruderInhibitory neurotransmissionChloride gradientChloride influxElegansCellsSevere disruptionCL flowNeural activityPrincipal mechanism
2010
Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct
Lu M, Dong K, Egan ME, Giebisch GH, Boulpaep EL, Hebert SC. Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6082-6087. PMID: 20231442, PMCID: PMC2851921, DOI: 10.1073/pnas.0902661107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoatesChloride ChannelsCyclic AMPCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorFemaleIn Vitro TechniquesKidney CortexKidney Tubules, CollectingKineticsMiceMice, Inbred C57BLMice, Inbred CFTRMice, KnockoutMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant ProteinsThiazolidinesXenopus laevisConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCl- channel activityConductance regulatorCl- channelsApical membrane proteinsExpression of CFTRChannel activityCFTR Cl- channelApical chloride channelApical cell membraneDeltaF508 CFTR mutationMembrane proteinsCatalytic subunitXenopus laevis oocytesForm proteinPrincipal cellsCFTR channelsROMK null miceApical patchesApical membraneSingle-channel conductanceChloride channelsCell membrane
2007
PI3 Kinase Dependent Stimulation of Gastric Acid Secretion by Dexamethasone
Lang P, Schniepp R, Kirchhoff P, Socrates T, Sidani S, Geibel J. PI3 Kinase Dependent Stimulation of Gastric Acid Secretion by Dexamethasone. Cellular Physiology And Biochemistry 2007, 20: 527-534. PMID: 17762179, DOI: 10.1159/000107536.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesAnimalsCimetidineDexamethasoneGastric AcidGastric MucosaH(+)-K(+)-Exchanging ATPaseMaleNitrobenzoatesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPotassium Channel BlockersProtein Kinase InhibitorsProton Pump InhibitorsRatsRats, Sprague-DawleyStaurosporineWortmanninConceptsGastric acid secretionProtein kinase inhibitor staurosporinePI3-kinase inhibitor wortmanninAcid secretionKinase inhibitor staurosporineKinase inhibitor wortmanninPI3-kinase pathwayPeptic ulcerDexamethasone effectApical Cl- channelsKinase pathwayInhibitor wortmanninInhibitor staurosporineExcessive gastric acid secretionProton extrusionATPase inhibitor omeprazoleCl- channel blockers NPPBCl- channelsChannel blocker NPPBDependent stimulationDexamethasone injectionVivo pretreatmentInhibitor omeprazoleParietal cellsSecretion
2006
ΔF508 Mutation Results in Impaired Gastric Acid Secretion*
Sidani SM, Kirchhoff P, Socrates T, Stelter L, Ferreira E, Caputo C, Roberts KE, Bell RL, Egan ME, Geibel JP. ΔF508 Mutation Results in Impaired Gastric Acid Secretion*. Journal Of Biological Chemistry 2006, 282: 6068-6074. PMID: 17178714, DOI: 10.1074/jbc.m608427200.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorATP-binding cassette (ABC) transportersFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorMouse gastric glandsParietal cellsMultifunctional proteinCFTR proteinRegulatory proteinsTransport proteinsCassette transportersConductance regulatorRegulatory roleApical poleSecretagogue-induced acid secretionGland lumenGastric glandsSulfonylurea receptorProteinImpaired gastric acid secretionK-ATPaseCl(-) secretionImmunofluorescent localizationCl- channelsATP-sensitive potassium channelsCFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney
Lu M, Leng Q, Egan ME, Caplan MJ, Boulpaep EL, Giebisch GH, Hebert SC. CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney. Journal Of Clinical Investigation 2006, 116: 797-807. PMID: 16470247, PMCID: PMC1361349, DOI: 10.1172/jci26961.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCurcuminCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorHydrogen-Ion ConcentrationKidneyMiceMice, Inbred C57BLMice, Inbred CFTRMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingXenopus laevisConceptsFunctional switchCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelATP sensitivityEffects of CFTRThick ascending limbPotential physiological rolePKA activityRenal K channelsCystic fibrosisPhysiological roleSecretory channelsK channelsRenal tubule epithelial cellsApical membraneCFTRDeltaF508 mutationDistal nephron segmentsCl- channelsK homeostasisTubule epithelial cellsEpithelial cellsTAL cellsPotassium channelsK handlingGlibenclamide sensitivity
2004
Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane
Vessey JP, Shi C, Jollimore CA, Stevens KT, Coca-Prados M, Barnes S, Kelly ME. Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane. Biochemistry And Cell Biology 2004, 82: 708-718. PMID: 15674438, DOI: 10.1139/o04-107.Peer-Reviewed Original ResearchConceptsPlasma membraneHyposmotic stimulationFluorescent membrane dye FM1-43Membrane dye FM1-43Trafficking of channelsClC-3 channelsCiliary epithelial cellsPhosphoinositide-3 kinase inhibitor wortmanninRate of exocytosisClC-3 Cl(-) channelsEpithelial cellsVesicular traffickingDye FM1-43Membrane dynamicsNonpigmented ciliary epithelial cellsInhibitor wortmanninClC-3 antisenseImmunofluorescence microscopyTraffickingClC-3Regulatory volume decreaseFM1-43Cl- channelsMembraneCell swelling
2003
Calcium-dependent, swelling-activated K+ conductance in human neuroblastoma cells
Basavappa S, Mangel A, Boulpaep E. Calcium-dependent, swelling-activated K+ conductance in human neuroblastoma cells. Biochemical And Biophysical Research Communications 2003, 308: 759-763. PMID: 12927783, DOI: 10.1016/s0006-291x(03)01481-5.Peer-Reviewed Original ResearchConceptsHuman neuroblastoma cell line CHP-100CHP-100Regulatory volume decreaseCell-attached patch-clamp studiesFree extracellular solutionHuman neuroblastoma cellsSwelling-activated ClPatch-clamp studiesFura-2Extracellular osmolarityFree extracellularNeuroblastoma cellsMOsm/Hypoosmotic solutionCell swellingExtracellular solutionCl- channelsHypoosmotic stressPresent studyMost mammalian cellsEGTACellsVolume decreaseEffluxPrevious studies
2001
PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells
Fleischhauer J, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2001, 281: c1614-c1623. PMID: 11600425, DOI: 10.1152/ajpcell.2001.281.5.c1614.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsCiliary epithelial cellsCl- channelsEpithelial cellsPE cellsCl- channel activityAction of nucleotidesProtein kinaseNonpigmented ciliary epithelial cellsSecond messengerCell sortingChannel activityATP activationElectronic cell sortingATPSequential cascadeChannel activationNucleotidesP2Y receptorsPatch clampingATP assayATP releaseCellsMicroM ATPCAMPMale germ cells and photoreceptors, both dependent on close cell–cell interactions, degenerate upon ClC‐2 Cl− channel disruption
Bösl M, Stein V, Hübner C, Zdebik A, Jordt S, Mukhopadhyay A, Davidoff M, Holstein A, Jentsch T. Male germ cells and photoreceptors, both dependent on close cell–cell interactions, degenerate upon ClC‐2 Cl− channel disruption. The EMBO Journal 2001, 20: 1289-1299. PMID: 11250895, PMCID: PMC145530, DOI: 10.1093/emboj/20.6.1289.Peer-Reviewed Original ResearchConceptsClC-2Abnormal Sertoli cellsBlood-retina barrierGerm cellsRetinal pigment epitheliumCl- channelsSevere degenerationMale infertilityPigment epitheliumMale germ cellsClC-2 Cl(-) channelOvert abnormalitiesSeminiferous tubulesNormal outer segmentsSertoli cellsOuter segmentsRetinaCell typesDeathMassive deathCellsPrimary spermatocytesHuman diseasesTubulesCLC Cl(-) channels
2000
Cl‐Dependent Na‐H Exchange: A Novel Colonic Crypt Transport Mechanism
BINDER H, RAJENDRAN V, GEIBEL J. Cl‐Dependent Na‐H Exchange: A Novel Colonic Crypt Transport Mechanism. Annals Of The New York Academy Of Sciences 2000, 915: 43-53. PMID: 11193600, DOI: 10.1111/j.1749-6632.2000.tb05222.x.Peer-Reviewed Original ResearchCa2+‐activated Cl− channels can substitute for CFTR in stimulation of pancreatic duct bicarbonate secretion
ZSEMBERY Á, Strazzabosco M, Graf J. Ca2+‐activated Cl− channels can substitute for CFTR in stimulation of pancreatic duct bicarbonate secretion. The FASEB Journal 2000, 14: 2345-2356. PMID: 11053257, DOI: 10.1096/fj.99-0509com.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateBicarbonatesCalciumCesiumChloride ChannelsChloridesCyclic AMPCystic Fibrosis Transmembrane Conductance RegulatorGlyburideGlycerolHumansHydrogen-Ion ConcentrationMembrane PotentialsMutationPancreatic DuctsPatch-Clamp TechniquesPotassium ChannelsTumor Cells, CulturedThe Na-K-Cl Cotransporter of Secretory Epithelia
Haas M, Forbush B. The Na-K-Cl Cotransporter of Secretory Epithelia. Annual Review Of Physiology 2000, 62: 515-534. PMID: 10845101, DOI: 10.1146/annurev.physiol.62.1.515.Peer-Reviewed Original ResearchConceptsNa-K-ClNa-K-Cl cotransporterRegulation of NKCC1Secretory epitheliaNa-K-Cl cotransporter isoformsK-Cl cotransporter isoformsApical Cl- channelsNa-Cl cotransporterNon-epithelial cellsNKCC1 regulationCl- channelsBasolateral membraneCotransporter proteinFluid secretionNKCC isoformsNKCC1Ascending limbHenle's loopEpithelial cellsMacula densaIon transport proteinsTransport NaEpitheliaCotransporterCell volume
1999
A3 adenosine receptors regulate Cl−channels of nonpigmented ciliary epithelial cells
Mitchell C, Peterson-Yantorno K, Carré D, McGlinn A, Coca-Prados M, Stone R, Civan M. A3 adenosine receptors regulate Cl−channels of nonpigmented ciliary epithelial cells. American Journal Of Physiology 1999, 276: c659-c666. PMID: 10069993, DOI: 10.1152/ajpcell.1999.276.3.c659.Peer-Reviewed Original ResearchConceptsAgonist N6HCE cellsIB-MECAA3 receptorsAdenosine receptorsCiliary processesA2A agonist CGS 21680Rabbit ciliary processesCiliary epitheliumA1-selective agonist N6Specific adenosine receptorsAgonist CGS 21680NPE cellsCl- channelsNonpigmented ciliary epithelial cellsA3 adenosine receptorHuman NPE cellsCGS 21680Ciliary epithelial cellsReceptor subtypesIntracellular Ca2MicroM adenosineRabbit ciliary epitheliumRT-PCREpithelial cellsCFTR Is a Conductance Regulator as well as a Chloride Channel
SCHWIEBERT E, BENOS D, EGAN M, STUTTS M, GUGGINO W. CFTR Is a Conductance Regulator as well as a Chloride Channel. Physiological Reviews 1999, 79: s145-s166. PMID: 9922379, DOI: 10.1152/physrev.1999.79.1.s145.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorConductance regulatorABC transportersCassette transporter gene familyCFTR Cl- channel functionTransporter gene familyFamily of transportersChloride channelsFibrosis transmembrane conductance regulatorCl- channel functionABC transporter familyTransmembrane conductance regulatorIon channel proteinsCystic fibrosis epitheliaGene familyCellular functionsCellular proteinsTransporter familyChannel proteinsCF geneAmino acidsIon channelsRegulatorTransportersCl- channelsRole of Cl channels in Cl-dependent Na/H exchange
Rajendran V, Geibel J, Binder H. Role of Cl channels in Cl-dependent Na/H exchange. American Journal Of Physiology 1999, 276: g73-g78. PMID: 9886981, DOI: 10.1152/ajpgi.1999.276.1.g73.Peer-Reviewed Original ResearchConceptsNa/H exchangeCl- channel blockerChannel blockersMicroM DIDSNa/H exchange activityCystic fibrosis transmembrane conductance regulatorIntracellular pH recoveryRat distal colonCl- channelsH exchange activityDistal colonCrypt lumenCrypt cellsAcid loadExchange inhibitorApical membranePH recoveryCl(-) dependenceAnion exchange inhibitorCl- channel activityChannel activityBlockersH exchangePolyclonal antibodiesCl- conductance
1998
ATP hydrolysis cycles and the gating of CFTR Cl- channels.
Gadsby D, Dousmanis A, Nairn A. ATP hydrolysis cycles and the gating of CFTR Cl- channels. Acta Physiologica Scandinavica. Supplementum 1998, 643: 247-56. PMID: 9789567.Peer-Reviewed Original ResearchConceptsC-terminal nucleotideCFTR channelsAMP-PNPG proteinsN-terminal nucleotideCentral regulatory domainMore serine residuesProtein kinase ACFTR Cl- channelHydrolysis of ATPATP hydrolysis cycleCl- channelsGating cycleRegulatory domainCytoplasmic domainTight bindingSerine residuesHydrolyse ATPSecond ATPSequence homologyTransport proteinsKinase AOpen conformationAnalogues of ATPFunctional similarityThe Na-K-Cl Cotransporters
Haas M, Forbush B. The Na-K-Cl Cotransporters. Journal Of Bioenergetics And Biomembranes 1998, 30: 161-172. PMID: 9672238, DOI: 10.1023/a:1020521308985.Peer-Reviewed Original ResearchConceptsNa-K-Cl cotransporterNa-K-ClNa-K-Cl cotransport activityNa-K-Cl cotransporter isoformsCotransport activitySecretory epitheliaApical Cl- channelsHypokalemic metabolic alkalosisCation-chloride cotransportersNKCC2 geneCl- channelsBartter's syndromeVolume depletionBasolateral membraneCotransporter proteinApical membraneSalt wastingMetabolic alkalosisSecreting epitheliaNonepithelial cellsAscending limbHenle's loopK-ClCotransporterEpithelial cellsChloride channel and chloride conductance regulator domains of CFTR, the cystic fibrosis transmembrane conductance regulator
Schwiebert E, Morales M, Devidas S, Egan M, Guggino W. Chloride channel and chloride conductance regulator domains of CFTR, the cystic fibrosis transmembrane conductance regulator. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 2674-2679. PMID: 9482946, PMCID: PMC19458, DOI: 10.1073/pnas.95.5.2674.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBase SequenceBronchiCells, CulturedChloride ChannelsChloridesCyclic AMPCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDNA, ComplementaryEpithelial CellsFemaleHumansMembrane PotentialsModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedOligodeoxyribonucleotidesOocytesPatch-Clamp TechniquesPoint MutationProtein ConformationRecombinant ProteinsSequence DeletionTranscription, GeneticTransfectionXenopus laevisConceptsCl- channel functionConductance regulatorDomains of CFTRCystic fibrosis transmembrane conductance regulatorChloride channelsFibrosis transmembrane conductance regulatorFirst transmembrane domainC-terminal truncationsIndividual amino acid substitutionsTransmembrane conductance regulatorCl- channel poreCl- channelsAmino acid substitutionsRegulator domainTransmembrane domainTwo-electrode voltage-clamp recordingsRegulatory domainMutant CFTRAcid substitutionsRegulator functionHuman airway epithelial cellsCFTRXenopus oocytesRegulatorRelease of ATP
1997
Ion exchangers mediating NaCl transport in the proximal tubule.
Aronson PS. Ion exchangers mediating NaCl transport in the proximal tubule. Wiener Klinische Wochenschrift 1997, 109: 435-40. PMID: 9261983.Commentaries, Editorials and LettersConceptsApical membraneBasolateral membraneBrush border membraneMembrane traffickingNHE3 protein expressionMembrane ion exchangerIntracellular vesiclesMammalian proximal tubuleBorder membraneDifferential regulationProximal tubule cellsInhibitor sensitivityPossible regulationFormate transportExchange activityProtein expressionCl- channelsTubule cellsMembranePolyclonal antibodiesVesiclesCellsNHE3Oxalate-sulfate exchangeRegulation
1996
Immunolocalization of ion transport proteins in human autosomal dominant polycystic kidney epithelial cells.
Brill SR, Ross KE, Davidow CJ, Ye M, Grantham JJ, Caplan MJ. Immunolocalization of ion transport proteins in human autosomal dominant polycystic kidney epithelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 10206-10211. PMID: 8816777, PMCID: PMC38362, DOI: 10.1073/pnas.93.19.10206.Peer-Reviewed Original ResearchConceptsCyst epithelial cellsEpithelial cellsCystic fibrosis transmembrane conductance regulatorIon transport proteinsFibrosis transmembrane conductance regulatorK-ATPaseApical cystic fibrosis transmembrane conductance regulatorTransmembrane conductance regulatorKidney epithelial cellsK-ATPase proteinRenal epithelial cellsCFTR proteinTransport proteinsConductance regulatorIntracellular structuresFluid-filled cystsApical membraneForskolin treatmentBasolateral surfacePermeable filter supportsApical surfaceProteinCl- channelsApical labelingAutosomal dominant polycystic kidney disease
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