2010
The Na+-dependent chloride-bicarbonate exchanger SLC4A8 mediates an electroneutral Na+ reabsorption process in the renal cortical collecting ducts of mice
Leviel F, Hübner CA, Houillier P, Morla L, Moghrabi S, Brideau G, Hatim H, Parker MD, Kurth I, Kougioumtzes A, Sinning A, Pech V, Riemondy KA, Miller RL, Hummler E, Shull GE, Aronson PS, Doucet A, Wall SM, Chambrey R, Eladari D. The Na+-dependent chloride-bicarbonate exchanger SLC4A8 mediates an electroneutral Na+ reabsorption process in the renal cortical collecting ducts of mice. Journal Of Clinical Investigation 2010, 120: 1627-1635. PMID: 20389022, PMCID: PMC2860930, DOI: 10.1172/jci40145.Peer-Reviewed Original ResearchConceptsNa-Cl cotransporterSodium transportMaintenance of euvolemiaTransepithelial NaCl absorptionDucts of miceEpithelial sodium channelIntravascular volumeIndependent Cl-/HCO3Sodium balanceMouse CCDAmiloride-sensitive epithelial sodium channelSodium absorptionExcretion resultsFluid homeostasisGenetic ablationNaCl absorptionSodium channelsMiceCl-/HCO3NaCl transportSlc4a8Genetic disruptionNovel roleHydrochlorothiazideDuct
2004
Renal and intestinal transport defects in Slc26a6-null mice
Wang Z, Wang T, Petrovic S, Tuo B, Riederer B, Barone S, Lorenz JN, Seidler U, Aronson PS, Soleimani M. Renal and intestinal transport defects in Slc26a6-null mice. American Journal Of Physiology - Cell Physiology 2004, 288: c957-c965. PMID: 15574486, DOI: 10.1152/ajpcell.00505.2004.Peer-Reviewed Original ResearchConceptsWild-type miceProximal tubulesSlc26a6-null miceHCO3- secretionKidney proximal tubulesApical membrane ClNull miceBaseline rateNormal blood pressureCl-/formate exchangeBlood pressureKidney functionElectrolyte profileMucosal tissuesIntestinal physiologyUssing chambersSmall intestineMiceFluid absorptionNaCl absorptionStatistical significanceCl-/HCO3NaCl transportDuodenumTubules
2003
Deficient HCO 3 - Transport in an AE1 Mutant with Normal Cl- Transport Can be Rescued by Carbonic Anhydrase II Presented on an Adjacent AE1 Protomer*
Dahl NK, Jiang L, Chernova MN, Stuart-Tilley AK, Shmukler BE, Alper SL. Deficient HCO 3 - Transport in an AE1 Mutant with Normal Cl- Transport Can be Rescued by Carbonic Anhydrase II Presented on an Adjacent AE1 Protomer*. Journal Of Biological Chemistry 2003, 278: 44949-44958. PMID: 12933803, DOI: 10.1074/jbc.m308660200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnion Exchange Protein 1, ErythrocyteAnionsBicarbonatesBinding SitesBiological TransportCarbonic Anhydrase IIChloridesDimerizationFemaleGene DeletionGene ExpressionHumansLipid BilayersMiceMolecular Sequence DataMutagenesisMutation, MissenseOocytesProtein SubunitsStructure-Activity RelationshipTransfectionXenopusConceptsCl-/HCO3- exchange activityPrevention of Ca2Normal CLCl-/HCO3II inhibitionHCO3- transportIntracellular HCO3Missense mutationsCytoplasmic tailC-terminal cytoplasmic tailXenopus oocytesCa2Binding sitesCl transportAE1InhibitionIntact Ca2Minimal extentCarbonic anhydrase IIActivityMissense mutantsPathwayAE1 mutantsExchange activity
2000
Na+/H+ and Cl–/HCO3–-antiporters of bovine pigmented ciliary epithelial cells
Counillon L, Touret N, Bidet M, Peterson-Yantorno K, Coca-Prados M, Stuart-Tilley A, Wilhelm S, Alper S, Civan M. Na+/H+ and Cl–/HCO3–-antiporters of bovine pigmented ciliary epithelial cells. Pflügers Archiv - European Journal Of Physiology 2000, 440: 667-678. PMID: 11007305, DOI: 10.1007/s004240000302.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnion Transport ProteinsAntiportersCattleCell Line, TransformedChloride-Bicarbonate AntiportersCiliary BodyEpithelial CellsImmunohistochemistryMembrane ProteinsMicroscopy, FluorescenceMicroscopy, VideoModels, BiologicalPigmentationReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSLC4A ProteinsSodiumSodium-Hydrogen ExchangersConceptsAqueous humor formationCl-/HCO3Epithelial cell layerTranscriptase-polymerase chain reaction amplificationPE cellsCell layerHuman ciliary bodyAE2 Cl-/HCO3Medical therapyNHE-1 isoformCiliary epithelial cellsCiliary bodyAqueous humorCiliary epithelial bilayerPolymerase chain reaction amplificationChain reaction amplificationNHE-1Rabbit ciliary epitheliumCiliary epitheliumEpithelial cellsBovine pigmented ciliary epithelial cellsGap junctionsRegulatory volume increasePigmented ciliary epithelial cellsExternal Cl
1999
Characterization of ion transport mechanisms involved in bombesin-stimulated biliary secretion in rat cholangiocytes
Cho W, Boyer J. Characterization of ion transport mechanisms involved in bombesin-stimulated biliary secretion in rat cholangiocytes. Journal Of Hepatology 1999, 30: 1045-1051. PMID: 10406182, DOI: 10.1016/s0168-8278(99)80258-x.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAcetazolamideAnimalsBarium CompoundsBileBile DuctsBombesinCarbonic Anhydrase InhibitorsChloride ChannelsChloridesIn Vitro TechniquesIon TransportMaleMicroscopy, VideoNitrobenzoatesPotassium ChannelsRatsRats, Sprague-DawleyTetraethylammonium
1998
Nitric oxide and guanosine 3′,5′‐cyclic monophosphate stimulate bile secretion in isolated rat hepatocyte couplets, but not in isolated bile duct units
Trauner M, Mennone A, Gigliozzi A, Fraioli F, Boyer J. Nitric oxide and guanosine 3′,5′‐cyclic monophosphate stimulate bile secretion in isolated rat hepatocyte couplets, but not in isolated bile duct units. Hepatology 1998, 28: 1621-1628. PMID: 9828227, DOI: 10.1002/hep.510280623.Peer-Reviewed Original ResearchConceptsDuctular bile secretionBile secretionBile duct unitsNitric oxideRat hepatocyte coupletsExchanger activityNO synthesisCl-/HCO3Bile acid-independent bile flowCell-permeant cGMP analogDuct unitsEndogenous NO synthesisHepatocyte coupletsEffect of NOCell-permeant cAMP analogsLevel of hepatocytesCyclic monophosphateHepatic NO synthesisRemoval of HCO3Canalicular bile secretionNG-monomethylBile flowCholeretic effectDuctular luminaL-arginineRenal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger
Schultheis P, Clarke L, Meneton P, Miller M, Soleimani M, Gawenis L, Riddle T, Duffy J, Doetschman T, Wang T, Giebisch G, Aronson P, Lorenz J, Shull G. Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger. Nature Genetics 1998, 19: 282-285. PMID: 9662405, DOI: 10.1038/969.Peer-Reviewed Original ResearchConceptsAcid-base balanceAbsorptive defectsNHE3-deficient miceFluid volume homeostasisRenal proximalSlight diarrheaPlasma aldosteroneBlood pressureHomozygous mutant miceExchanger mRNANHE2 isoformsBlood analysisMutant miceCompensatory mechanismsFluid absorptionNHE3 functionVolume homeostasisMiceDistribution of NHE3Cl-/HCO3Channel activityNHE3KidneyATPase mRNAIntestine
1994
Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes.
Benedetti A, Strazzabosco M, Ng O, Boyer J. Regulation of activity and apical targeting of the Cl-/HCO3- exchanger in rat hepatocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 792-796. PMID: 8290601, PMCID: PMC43035, DOI: 10.1073/pnas.91.2.792.Peer-Reviewed Original ResearchConceptsApical targetingExchange activityRegulation of activityMembrane localizationCl-/HCO3Dibutyryl cAMPProtein kinase C agonistsRat hepatocytesProtein kinase C agonist phorbolEcto-ATPaseConfocal microscopyCanalicular domainC agonistsProteinVesiclesTargetingCanalicular localizationLocalizationCAMPHepatocyte coupletsHepatocytesActivityRegulationPhorbolColchicine
1993
Effect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells.
Alvaro D, Cho W, Mennone A, Boyer J. Effect of secretion on intracellular pH regulation in isolated rat bile duct epithelial cells. Journal Of Clinical Investigation 1993, 92: 1314-1325. PMID: 8397224, PMCID: PMC288273, DOI: 10.1172/jci116705.Peer-Reviewed Original ResearchConceptsBile duct epithelial cellsDuct epithelial cellsCl-/HCO3Cell depolarizationEpithelial cellsEffect of secretinCl- channel blockerEffects of secretionsCl removalCl- channel activationRat bile duct epithelial cellsKRB mediumMaximal rateRegulation of intracellularBicarbonate secretionNormal rat liverIntracellular pH regulationMM DIDSHCO3- excretionAcid loadBDE cellsSecretinMicrofluorimetric methodRat liverHCO3- influx
1989
Bicarbonate-dependent and -independent intracellular pH regulatory mechanisms in rat hepatocytes. Evidence for Na+-HCO3- cotransport.
Gleeson D, Smith N, Boyer J. Bicarbonate-dependent and -independent intracellular pH regulatory mechanisms in rat hepatocytes. Evidence for Na+-HCO3- cotransport. Journal Of Clinical Investigation 1989, 84: 312-321. PMID: 2544626, PMCID: PMC303984, DOI: 10.1172/jci114156.Peer-Reviewed Original ResearchConceptsDisulfonic acid stilbeneHCO3- cotransportIntracellular acid loadHCO3- thanIntracellular pH regulatory mechanismsPH regulatory mechanismsBaseline pHiMonolayer cell culture systemRat hepatocytesAcid loadIntracellular ClCl-/HCO3AmilorideCell culture systemAnion transport inhibitorAbsence of bicarbonateHCO3- transportImportant regulatorHepatocytesPH-sensitive dyeCarboxy-fluoresceinTransport inhibitorsCulture systemRegulatory mechanismsPhi
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