2024
A safe insect-based chikungunya fever vaccine affords rapid and durable protection in cynomolgus macaques
Adam A, Woolsey C, Lu H, Plante K, Wallace S, Rodriguez L, Shinde D, Cui Y, Franz A, Thangamani S, Comer J, Weaver S, Wang T. A safe insect-based chikungunya fever vaccine affords rapid and durable protection in cynomolgus macaques. Npj Vaccines 2024, 9: 251. PMID: 39702442, PMCID: PMC11659317, DOI: 10.1038/s41541-024-01047-z.Peer-Reviewed Original ResearchMemory B cellsCynomolgus macaquesB cellsDose vaccinationAntigen presenting cell activationReceptor signalingCHIK feverNK cell responsesRobust type I interferonT cell responsesT cell immunityMultiple innate immune pathwaysToll-like receptor signalingWild-typeDose-dependent mannerSkin hypersensitivity reactionsType I interferonInnate immune signalingInnate immune pathwaysT cellsMechanisms of host protectionCHIKV infectionCell activationHypersensitivity reactionsAntibody response
2023
Multicenter study of the natural history and therapeutic responses of patients with chikungunya, focusing on acute and chronic musculoskeletal manifestations – a study protocol from the clinical and applied research in Chikungunya (REPLICK network)
da Silva Duarte G, Jones A, de Goes Cavalcanti L, de Melo Rêgo M, Ribeiro G, Boyton R, Pereira D, Croda J, Costa F, Duarte A, Consolaro M, Stabeli R, Negrão F, Proenca-Modena J, Villalobos-Salcedo J, da Rocha Castelar Pinheiro G, de Barros Albuquerque A, de Almeida Barreto F, Moreira J, Ferrari I, Évora P, da Silva V, Lacerda M, Altmann D, Siqueira A. Multicenter study of the natural history and therapeutic responses of patients with chikungunya, focusing on acute and chronic musculoskeletal manifestations – a study protocol from the clinical and applied research in Chikungunya (REPLICK network). BMC Infectious Diseases 2023, 23: 499. PMID: 37507666, PMCID: PMC10386654, DOI: 10.1186/s12879-023-08292-y.Peer-Reviewed Original ResearchConceptsMusculoskeletal manifestationsStudy protocolTherapeutic responseChronic diseasesNatural historyDestructive disease courseMulticenter cohort studyLong-term sequelaeAssociated risk factorsClinical report formsClinical trial designQuality of lifeAliquots of bloodCohort studyCHIKV infectionDisease courseChronic painSymptomatic infectionBlood testsHigh morbidityMulticenter studyMusculoskeletal symptomsTherapeutic guidelinesClinical spectrumPatient cohort
2022
Interleukin-17 Contributes to Chikungunya Virus-Induced Disease
Liu X, Poo YS, Alves JC, Almeida RP, Mostafavi H, Tang PCH, Bucala R, Teixeira MM, Taylor A, Zaid A, Mahalingam S. Interleukin-17 Contributes to Chikungunya Virus-Induced Disease. MBio 2022, 13: e00289-22. PMID: 35254128, PMCID: PMC9040832, DOI: 10.1128/mbio.00289-22.Peer-Reviewed Original ResearchConceptsIL-17Chikungunya virusCHIKV infectionRheumatoid arthritisIL-17-deficient miceIL-17A-deficient miceInterleukin-17 ContributesSpecific antiviral drugsWild-type miceIL-17 signalingAlphaviral arthritidesIL-17AIL-23Neutrophil infiltrationPsoriatic arthritisArthritic inflammationIL-21Acute phaseIL-22Interleukin-17Chronic phaseIL-6Tissue infiltratesTissue inflammationDisease patients
2021
Acute-Phase Levels of CXCL8 as Risk Factor for Chronic Arthralgia Following Chikungunya Virus Infection
Jacob-Nascimento L, Carvalho C, Silva M, Kikuti M, Anjos R, Fradico J, Campi-Azevedo A, Tauro L, Campos G, dos Santos Moreira P, Portilho M, Martins-Filho O, Ribeiro G, Reis M. Acute-Phase Levels of CXCL8 as Risk Factor for Chronic Arthralgia Following Chikungunya Virus Infection. Frontiers In Immunology 2021, 12: 744183. PMID: 34659240, PMCID: PMC8517435, DOI: 10.3389/fimmu.2021.744183.Peer-Reviewed Original ResearchConceptsChikungunya virus infectionCHIKV infectionHealthy controlsIL-12IL-1βVirus infectionFlow cytometric bead arrayAcute phase immune responseAcute CHIKV infectionAnti-CHIKV IgMAcute-phase samplesCytometric bead arrayAcute febrile diseaseAcute-phase levelsCHIKV patientsSerum chemokinesCXCL10 levelsPain persistenceIL-10Cytokine kineticsMultivariable analysisCXCL8 levelsIL-6Female sexIL-2
2020
Macrophage scavenger receptor 1 controls Chikungunya virus infection through autophagy in mice
Yang L, Geng T, Yang G, Ma J, Wang L, Ketkar H, Yang D, Lin T, Hwang J, Zhu S, Wang Y, Dai J, You F, Cheng G, Vella AT, Flavell RA, Fikrig E, Wang P. Macrophage scavenger receptor 1 controls Chikungunya virus infection through autophagy in mice. Communications Biology 2020, 3: 556. PMID: 33033362, PMCID: PMC7545163, DOI: 10.1038/s42003-020-01285-6.Peer-Reviewed Original ResearchConceptsMacrophage scavenger receptor 1Scavenger receptor 1Chikungunya virusReceptor 1Antiviral roleType I IFN responseChikungunya virus infectionLow-density lipoproteinImportant antiviral roleI IFN responseMarkers of autophagyCHIKV infectionViral loadArthritogenic alphavirusesVirus infectionCHIKV replicationATG5-ATG12Antiviral actionKnockout miceMSR1 expressionIFN responseInfectionMiceNsp1 proteinAutophagic functionClinical Characteristics, Histopathology, and Tissue Immunolocalization of Chikungunya Virus Antigen in Fatal Cases
Sharp T, Keating M, Shieh W, Bhatnagar J, Bollweg B, Levine R, Blau D, Torres J, Rivera A, Perez-Padilla J, Munoz-Jordan J, Sanabria D, Fischer M, Garcia B, Tomashek K, Zaki S. Clinical Characteristics, Histopathology, and Tissue Immunolocalization of Chikungunya Virus Antigen in Fatal Cases. Clinical Infectious Diseases 2020, 73: e345-e354. PMID: 32615591, PMCID: PMC11307670, DOI: 10.1093/cid/ciaa837.Peer-Reviewed Original ResearchConceptsSeptic shockFatal casesTissue specimensMononuclear cellsSplenic follicular dendritic cellsChikungunya virus antigenIntra-alveolar hemorrhageFatal septic shockFollicular dendritic cellsChikungunya virus infectionCommon histopathologic findingsBlood mononuclear cellsReverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionCHIKV antigenSevere comorbiditiesAcute illnessClinical characteristicsCHIKV infectionDendritic cellsMedian ageRecord reviewIllness onsetImmunohistochemical findingsHistopathologic findingsQuantitative Proteomic Analysis of Chikungunya Virus-Infected Aedes aegypti Reveals Proteome Modulations Indicative of Persistent Infection
Cui Y, Liu P, Mooney BP, Franz AWE. Quantitative Proteomic Analysis of Chikungunya Virus-Infected Aedes aegypti Reveals Proteome Modulations Indicative of Persistent Infection. Journal Of Proteome Research 2020, 19: 2443-2456. PMID: 32375005, PMCID: PMC7419016, DOI: 10.1021/acs.jproteome.0c00173.Peer-Reviewed Original ResearchConceptsSerine-type endopeptidasesMetabolism related pathwaysQuantitative proteomic analysisFunctional enrichment analysisChikungunya virusRibosome biogenesisLabel-free quantificationRNA machineryNonpathogenic relationshipsNovel proteinProteome modulationProteomic analysisVesicular transportEnrichment analysisCHIKV infectionOxidative phosphorylationBiological pathwaysRelated pathwaysMass spectrometry dataPresence of CHIKVProteinMosquito samplesProteomeOral acquisitionMolecular interactions
2019
Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses
Kikuti M, Tauro L, Moreira P, Nascimento L, Portilho M, Soares G, Weaver S, Reis M, Kitron U, Ribeiro G. Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. International Journal Of Infectious Diseases 2019, 91: 38-43. PMID: 31704226, DOI: 10.1016/j.ijid.2019.11.001.Peer-Reviewed Original ResearchConceptsConvalescent samplesIgM enzymeRT-PCRZika virus transmissionNegative patientsCHIKV infectionAcute samplesZIKV casesConvalescent seraSurveillance studyCHIKV transmissionZika virusCHIKV casesDiagnostic performanceLow specificityVirus transmissionEquivocal resultsTest sensitivitySerumELISA specificityPatientsHigh rateDengueConcomitant transmissionMore equivocal results
2018
Conserved motifs in the hypervariable domain of chikungunya virus nsP3 required for transmission by Aedes aegypti mosquitoes
Göertz GP, Lingemann M, Geertsema C, Abma-Henkens MHC, Vogels CBF, Koenraadt CJM, van Oers MM, Pijlman GP. Conserved motifs in the hypervariable domain of chikungunya virus nsP3 required for transmission by Aedes aegypti mosquitoes. PLOS Neglected Tropical Diseases 2018, 12: e0006958. PMID: 30412583, PMCID: PMC6249005, DOI: 10.1371/journal.pntd.0006958.Peer-Reviewed Original ResearchConceptsCHIKV replicationChikungunya virusAedes aegypti mosquitoesMosquito cellsAegypti mosquitoesTransmission of CHIKVCHIKV infectionHypervariable domainMosquito salivaHost proteinsInfectionCHIKV mutantsChikungunya Virus nsP3Non-structural proteinsCHIKV nsP3Arthropod-borneEfficient infectionIntervention strategiesViral RNATransmission cycleMosquitoesFGDF motifsCellsMammalian cellsRich motif
2017
Seroprevalence of Chikungunya Virus in a Rural Community in Brazil
Cunha R, Trinta K, Montalbano C, Sucupira M, de Lima M, Marques E, Romanholi I, Croda J. Seroprevalence of Chikungunya Virus in a Rural Community in Brazil. PLOS Neglected Tropical Diseases 2017, 11: e0005319. PMID: 28107342, PMCID: PMC5287455, DOI: 10.1371/journal.pntd.0005319.Peer-Reviewed Original ResearchConceptsCases of chikungunyaCHIKV infectionChikungunya virusCHIKV IgMSymptomatic CHIKV infectionIgM-specific antibodiesFeira de SantanaIgG-specific antibodiesEpidemiological surveillance systemBrazilian Epidemiological Surveillance SystemCHIKV IgGModerate seroprevalenceSeropositive patientsJoint painIgG antibodiesCHIKV transmissionFirst serosurveySeroprevalenceInfectionMagnitude of transmissionAntibodiesPatientsFirst caseSerosurveyIgM
2016
Use of Household Cluster Investigations to Identify Factors Associated with Chikungunya Virus Infection and Frequency of Case Reporting in Puerto Rico
Bloch D, Roth NM, Caraballo EV, Muñoz-Jordan J, Hunsperger E, Rivera A, Pérez-Padilla J, Garcia B, Sharp TM. Use of Household Cluster Investigations to Identify Factors Associated with Chikungunya Virus Infection and Frequency of Case Reporting in Puerto Rico. PLOS Neglected Tropical Diseases 2016, 10: e0005075. PMID: 27764085, PMCID: PMC5072658, DOI: 10.1371/journal.pntd.0005075.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAdolescentAdultAgedAged, 80 and overAir ConditioningAntibodies, ViralChikungunya FeverChikungunya virusChildChild, PreschoolCymbopogonDisease NotificationDisease OutbreaksEnzyme-Linked Immunosorbent AssayExanthemaFamily CharacteristicsFemaleFeverHumansInfantMaleMiddle AgedPoint-of-Care SystemsPolymerase Chain ReactionPuerto RicoYoung AdultConceptsSymptomatic CHIKV infectionRecent CHIKV infectionCHIKV infectionPublic health authoritiesChikungunya virusChikungunya patientsMedical careHealth authoritiesCase reportingAnti-CHIKV IgMAcute febrile illnessChikungunya virus infectionCare rapid diagnostic testsRapid diagnostic testsPuerto Rico DepartmentCluster investigationsAcute illnessFebrile illnessSkin rashResidents of householdsDevelopment of diseaseMultivariable analysisIllness onsetIgG antibodiesIgM ELISA
2015
Molecular Characterisation of Chikungunya Virus Infections in Trinidad and Comparison of Clinical and Laboratory Features with Dengue and Other Acute Febrile Cases
Sahadeo N, Mohammed H, Allicock O, Auguste A, Widen S, Badal K, Pulchan K, Foster J, Weaver S, Carrington C. Molecular Characterisation of Chikungunya Virus Infections in Trinidad and Comparison of Clinical and Laboratory Features with Dengue and Other Acute Febrile Cases. PLOS Neglected Tropical Diseases 2015, 9: e0004199. PMID: 26580074, PMCID: PMC4651505, DOI: 10.1371/journal.pntd.0004199.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overChikungunya FeverChikungunya virusDengueDiagnosis, DifferentialFemaleFever of Unknown OriginGenotypeHumansMaleMiddle AgedMolecular Diagnostic TechniquesMolecular Sequence DataPhylogenyRNA, ViralSequence Analysis, DNASequence HomologyTrinidad and TobagoYoung AdultConceptsAcute undifferentiated febrile illnessChikungunya feverChikungunya virusCHIKV infectionDengue virusDengue feverRT-qPCRExact testLower white blood cell countWhite blood cell countPearson χ2Undifferentiated febrile illnessCharacteristics of patientsChikungunya virus infectionSymptoms of peopleBlood cell countComparison of ClinicalConfidence intervalsFisher's exact testT-testStudent's t-testMann-Whitney UFebrile illnessJoint painLaboratory features
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