2008
Hypoxia-Inducible Factor-Dependent Degeneration, Failure, and Malignant Transformation of the Heart in the Absence of the von Hippel-Lindau Protein
Lei L, Mason S, Liu D, Huang Y, Marks C, Hickey R, Jovin IS, Pypaert M, Johnson RS, Giordano FJ. Hypoxia-Inducible Factor-Dependent Degeneration, Failure, and Malignant Transformation of the Heart in the Absence of the von Hippel-Lindau Protein. Molecular And Cellular Biology 2008, 28: 3790-3803. PMID: 18285456, PMCID: PMC2423296, DOI: 10.1128/mcb.01580-07.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCapillariesCell Transformation, NeoplasticErbB ReceptorsGene DeletionGene Transfer TechniquesHeart FailureHeart NeoplasmsHypoxiaHypoxia-Inducible Factor 1, alpha SubunitLipid MetabolismLipidsMiceMice, KnockoutMyocardiumNeovascularization, PhysiologicPhosphorylationProto-Oncogene Proteins c-metRas ProteinsVon Hippel-Lindau Tumor Suppressor ProteinConceptsHeart failureVon Hippel-Lindau proteinChronic activationAdvanced ischemic heart diseaseMalignant cardiac tumorsHIF-1alphaProgressive heart failureIschemic heart diseaseCardiac myocyte-specific deletionHIF pathwayHuman heart failureFeatures of rhabdomyosarcomaHypoxia-inducible factorHypoxia-inducible transcription factor-1Cardiac tumorsHeart diseaseTranscription factor 1Cardiac degenerationIschemic heartMyocyte lossDependent degenerationMalignant transformationPremature deathLipid accumulationCardiac muscle
2006
An engineered VEGF‐activating zinc finger protein transcription factor improves blood flow and limb salvage in advanced‐age mice
Yu J, Lei L, Liang Y, Hinh L, Hickey RP, Huang Y, Liu D, Yeh JL, Rebar E, Case C, Spratt K, Sessa WC, Giordano FJ. An engineered VEGF‐activating zinc finger protein transcription factor improves blood flow and limb salvage in advanced‐age mice. The FASEB Journal 2006, 20: 479-481. PMID: 16423874, DOI: 10.1096/fj.04-3670fje.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAgingAmino Acid SequenceAnimalsBlood Flow VelocityFeasibility StudiesGene Expression RegulationGenes, SyntheticGenetic TherapyGenetic VectorsHindlimbIschemiaLaser-Doppler FlowmetryMiceMice, Inbred C57BLMolecular Sequence DataNeovascularization, PhysiologicProtein EngineeringRecombinant ProteinsRNA, MessengerStructure-Activity RelationshipTranscription FactorsVascular Endothelial Growth Factor AZinc FingersConceptsLimb salvageBlood flowHindlimb ischemiaC57/BL6 micePeripheral vascular diseaseVascular endothelial growth factorPotential clinical utilityEndothelial growth factorExpression of VEGFABL6 miceIschemic limbsVascular diseaseIschemic hindlimbMurine modelClinical utilityVessel countProtein transcription factorsGrowth factorProtein levelsSalvage
2004
Endothelial-specific expression of caveolin-1 impairs microvascular permeability and angiogenesis
Bauer PM, Yu J, Chen Y, Hickey R, Bernatchez PN, Looft-Wilson R, Huang Y, Giordano F, Stan RV, Sessa WC. Endothelial-specific expression of caveolin-1 impairs microvascular permeability and angiogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 102: 204-209. PMID: 15615855, PMCID: PMC544041, DOI: 10.1073/pnas.0406092102.Peer-Reviewed Original ResearchConceptsEndothelial-specific expressionCav-1Function of caveolaeMitogen-activated protein kinaseCaveolin-1 geneAkt-Endothelial Nitric Oxide Synthase PathwayCaveolae biogenesisPhosphorylation of AktOrganelle numberProtein kinaseCav-1 expressionCaveolae numberEndothelial barrier functionSynthase pathwayEssential roleEndothelial nitric oxide synthase activationExogenous VEGFPhosphorylationCritical roleNitric oxide synthase activationAngiogenic responseSynthase activationTransgenic miceExpressionPathway
2002
Induction of angiogenesis in a mouse model using engineered transcription factors
Rebar EJ, Huang Y, Hickey R, Nath AK, Meoli D, Nath S, Chen B, Xu L, Liang Y, Jamieson AC, Zhang L, Spratt SK, Case CC, Wolffe A, Giordano FJ. Induction of angiogenesis in a mouse model using engineered transcription factors. Nature Medicine 2002, 8: 1427-1432. PMID: 12415262, DOI: 10.1038/nm1202-795.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAngiogenesis Inducing AgentsAnimalsDrug DesignGene Expression RegulationGenetic TherapyMiceModels, AnimalMolecular Sequence DataNeovascularization, PhysiologicProtein EngineeringRecombinant ProteinsTranscription FactorsVascular Endothelial Growth Factor AZinc FingersConceptsTranscription factorsEndogenous genesZinc finger protein transcription factorsProtein transcription factorsWhole-organism modelDNA sequencesInduced expressionGenesInduction of angiogenesisZFPExpression of VEGFAProtein VEGFExpressionGrowth factorStimulation of angiogenesisTissue cultureVascular endothelial growth factorExperimental wound healingEndothelial growth factorWound healingNatural arraysAngiogenesisVivoCDNAMouse modelCorticotropin-releasing factor receptor 2 is a tonic suppressor of vascularization
Bale TL, Giordano FJ, Hickey RP, Huang Y, Nath AK, Peterson KL, Vale WW, Lee KF. Corticotropin-releasing factor receptor 2 is a tonic suppressor of vascularization. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 7734-7739. PMID: 12032352, PMCID: PMC124337, DOI: 10.1073/pnas.102187099.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenesis InhibitorsAnimalsCapillariesCell DivisionCells, CulturedEndothelial Growth FactorsEndothelium, VascularGene Expression RegulationLymphokinesMiceMice, KnockoutMuscle, Smooth, VascularNeovascularization, PhysiologicReceptors, Corticotropin-Releasing HormoneVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsCorticotropin-releasing factor receptor 2Smooth muscle cellsCapillary tube formationTube formationCell cycle progressionVascular endothelial growth factorFactor receptor 2Protein phosphorylationRetinoblastoma proteinCycle progressionLigand activationReceptor 2Adult neovascularizationCRFR2-deficient miceCell proliferationIschemic cardiovascular diseasePotential targetAdult vesselsQuiescent stateMuscle cellsEndothelial growth factorGrowth factorSMC proliferationWestern blotCollagen gels