Kirill Volynski
Associate Professor AdjunctCards
About
Research
Publications
2025
Astrocyte Kir4.1 expression level territorially controls excitatory transmission in the brain
Tyurikova O, Kopach O, Zheng K, Rathore D, Codadu N, Wu S, Shen Y, Campbell R, Wykes R, Volynski K, Savtchenko L, Rusakov D. Astrocyte Kir4.1 expression level territorially controls excitatory transmission in the brain. Cell Reports 2025, 44: 115299. PMID: 39951378, DOI: 10.1016/j.celrep.2025.115299.Peer-Reviewed Original ResearchConceptsKir4.1 channelsExtracellular potassiumInducing long-term synaptic potentiationHigh-frequency afferent stimulationLong-term synaptic potentiationPresynaptic Ca<sup>2+</sup> entryCortical spreading depolarizationKir4.1 levelsExcitatory transmissionSynaptic potentialsAfferent stimulationExcitatory synapsesGlutamate uptakePotassium homeostasisSpreading depolarizationPotassium levelsRelease probabilityAstrocytic mechanismsAstrocytesHigh potassiumKir4.1Brain circuitsSynapsesBrainSynaptic Function
Kullmann D, Volynski K. Synaptic Function. 2025, 265-296. DOI: 10.1093/med/9780190065324.003.0006.Peer-Reviewed Original Research
2024
Minimal presynaptic protein machinery governing diverse kinetics of calcium-evoked neurotransmitter release
Bose D, Bera M, Norman C, Timofeeva Y, Volynski K, Krishnakumar S. Minimal presynaptic protein machinery governing diverse kinetics of calcium-evoked neurotransmitter release. Nature Communications 2024, 15: 10741. PMID: 39738049, PMCID: PMC11685451, DOI: 10.1038/s41467-024-54960-1.Peer-Reviewed Original ResearchConceptsSynaptotagmin-7Synaptotagmin-1Protein machinerySNARE complex assemblyFusion clampExocytosis processVesicle fusionVesicular fusionComplex assemblySynaptic vesiclesFusion assayMolecular basisPhysiologically relevant conditionsPresynaptic calcium influxNeurotransmitter releaseVesiclesSNAREProteinMachineryCompetitive bindingFusion dynamicsComplexinExocytosisFusionCalcium influxInvestigating Presynaptic Activity using the SF-iGluSnFR Sensor: Measuring Kinetics and Plasticity of Glutamate Release Across Heterogeneous Population of Synapses
Mendonça P, Langley H, Tagliatti E, Kotzadimitriou D, Timofeeva Y, Volynski K. Investigating Presynaptic Activity using the SF-iGluSnFR Sensor: Measuring Kinetics and Plasticity of Glutamate Release Across Heterogeneous Population of Synapses. Neuromethods 2024, 209: 295-319. DOI: 10.1007/978-1-0716-4011-1_14.Peer-Reviewed Original ResearchSingle-synapse levelStudy molecular mechanismsVesicular neurotransmitter releaseVesicular contentsNeurotransmitter releaseFunctional dynamicsCentral synapsesVesicular glutamate releaseRelease probabilityGlutamate releasePopulations of synapsesSynaptic plasticityShort-term plasticitySynapsesHeterogeneous populationRelease eventsPatch-clamp electrophysiologyFunctional propertiesMultiple synapsesPresynaptic activityGlutamate release eventsGlutamate sensorFluorescence imagingPlasticityAxonal arborsSynaptotagmin-1 and synaptotagmin-7 synergistically regulate the timing and plasticity of Ca2+-evoked vesicular release process
Bose D, Bera M, Norman C, Volynski K, Krishnakumar S. Synaptotagmin-1 and synaptotagmin-7 synergistically regulate the timing and plasticity of Ca2+-evoked vesicular release process. Biophysical Journal 2024, 123: 381a. DOI: 10.1016/j.bpj.2023.11.2327.Peer-Reviewed Original Research