2021
Probing Microbiome Genotoxicity: A Stable Colibactin Provides Insight into Structure–Activity Relationships and Facilitates Mechanism of Action Studies
Wernke KM, Tirla A, Xue M, Surovtseva YV, Menges FS, Herzon SB. Probing Microbiome Genotoxicity: A Stable Colibactin Provides Insight into Structure–Activity Relationships and Facilitates Mechanism of Action Studies. Journal Of The American Chemical Society 2021, 143: 15824-15833. PMID: 34524796, DOI: 10.1021/jacs.1c07559.Peer-Reviewed Original ResearchConceptsFanconi anemia DNA repair pathwayBiosynthetic gene clusterDNA repair pathwaysGene clusterRepair pathwaysDNA bindingBacterial phenotypesInduces DNAGenotoxic effectsHuman microbiomeColibactinBiosynthetic precursorNatural product structuresBiological studiesPathwayStructure-activity relationshipsAction studiesGenotoxic metabolitesOrganismsOncogenesisDNATumorigenesisMicrobiomeFacilitate mechanismsPhenotype
2020
Screening Novel Agent Combinations to Expedite CTCL Therapeutic Development
Mirza FN, Yumeen S, Lewis JM, King ALO, Kim S, Carlson KR, Umlauf S, Surovtseva YV, Foss FM, Girardi M. Screening Novel Agent Combinations to Expedite CTCL Therapeutic Development. Journal Of Investigative Dermatology 2020, 141: 217-221. PMID: 32534802, DOI: 10.1016/j.jid.2020.05.097.Peer-Reviewed Original ResearchJAK inhibition synergistically potentiates BCL2, BET, HDAC, and proteasome inhibition in advanced CTCL
Yumeen S, Mirza FN, Lewis JM, King ALO, Kim SR, Carlson KR, Umlauf SR, Surovtseva YV, Foss FM, Girardi M. JAK inhibition synergistically potentiates BCL2, BET, HDAC, and proteasome inhibition in advanced CTCL. Blood Advances 2020, 4: 2213-2226. PMID: 32437546, PMCID: PMC7252559, DOI: 10.1182/bloodadvances.2020001756.Peer-Reviewed Original ResearchConceptsCutaneous T-cell lymphomaJAK inhibitionCTCL cellsMalignant cutaneous T-cell lymphomasAdvanced cutaneous T-cell lymphomaTreatment of CTCLAvailable systemic treatment optionsSkin-homing T lymphocytesSystemic treatment optionsT-cell lymphomaCTCL cell linesHistone deacetylase inhibitionGeneralized cytotoxic effectExpression of Bcl2Advanced diseaseSuch patientsPeripheral bloodTreatment optionsJAK/STAT pathwayT lymphocytesPreclinical assessmentTherapeutic targetStrong potentiationExtrinsic apoptosis pathwayDeacetylase inhibition
2018
Model Colibactins Exhibit Human Cell Genotoxicity in the Absence of Host Bacteria
Shine EE, Xue M, Patel JR, Healy AR, Surovtseva YV, Herzon SB, Crawford JM. Model Colibactins Exhibit Human Cell Genotoxicity in the Absence of Host Bacteria. ACS Chemical Biology 2018, 13: 3286-3293. PMID: 30403848, PMCID: PMC7001666, DOI: 10.1021/acschembio.8b00714.Peer-Reviewed Original ResearchConceptsHost bacteriaGenotoxic secondary metabolitesDNA double-strand breaksDNA interstrandSpecific protein domainsWild-type pathwayDouble-strand breaksFull molecular mechanismsCell culturesFamily of metabolitesHuman cell linesProtein domainsPresence of membranesModule skippingCellular phenotypesExtracellular supplementationNative pathwaysHuman cell culturesMolecular mechanismsSecondary metabolitesHuman cellsColibactinEfficient DNA interstrandObserved modulesPhenotype
2009
Conserved Telomere Maintenance Component 1 Interacts with STN1 and Maintains Chromosome Ends in Higher Eukaryotes
Surovtseva Y, Churikov D, Boltz K, Song X, Lamb J, Warrington R, Leehy K, Heacock M, Price C, Shippen D. Conserved Telomere Maintenance Component 1 Interacts with STN1 and Maintains Chromosome Ends in Higher Eukaryotes. Molecular Cell 2009, 36: 207-218. PMID: 19854131, PMCID: PMC2768651, DOI: 10.1016/j.molcel.2009.09.017.Peer-Reviewed Original ResearchMeSH KeywordsAnaphaseArabidopsisArabidopsis ProteinsCell Line, TumorChromosomal Proteins, Non-HistoneChromosomes, PlantConserved SequenceEukaryotic CellsGenomic InstabilityHumansIn Situ Hybridization, FluorescenceMutationNucleic Acid ConformationProtein BindingRecombination, GeneticTelomereTelomere-Binding ProteinsConceptsG-overhangsOB-fold domainDNA damage responseArabidopsis mutantsMulticellular organismsHigher eukaryotesTEN1 (CST) complexChromosome endsHuman CTC1Telomere integrityTelomere maintenanceDiverse speciesDamage responseEnd fusionsChromatin bridgesTelomere lossRNAi triggersDevelopmental defectsCTC1Stn1PlantsEukaryotesOrthologsVertebratesMutants
2005
The Arabidopsis Pot1 and Pot2 Proteins Function in Telomere Length Homeostasis and Chromosome End Protection
Shakirov E, Surovtseva Y, Osbun N, Shippen D. The Arabidopsis Pot1 and Pot2 Proteins Function in Telomere Length Homeostasis and Chromosome End Protection. Molecular And Cellular Biology 2005, 25: 7725-7733. PMID: 16107718, PMCID: PMC1190295, DOI: 10.1128/mcb.25.17.7725-7733.2005.Peer-Reviewed Original ResearchConceptsChromosome end protectionTelomere length homeostasisEnd protectionLength homeostasisGenome instabilityWild typePot1-like proteinsSevere growth defectOligonucleotide/oligosaccharideMassive genome instabilityBulk telomeresTransgenic plantsGrowth defectProtein functionPositive regulationN-terminusBinding proteinAnalogous regionMutantsArabidopsisPlantsLength controlCorresponding regionPOT1Protein
2004
A C-terminal Myb Extension Domain Defines a Novel Family of Double-strand Telomeric DNA-binding Proteins in Arabidopsis *
Karamysheva Z, Surovtseva Y, Vespa L, Shakirov E, Shippen D. A C-terminal Myb Extension Domain Defines a Novel Family of Double-strand Telomeric DNA-binding Proteins in Arabidopsis *. Journal Of Biological Chemistry 2004, 279: 47799-47807. PMID: 15364931, DOI: 10.1074/jbc.m407938200.Peer-Reviewed Original ResearchConceptsPlant telomeric DNATelomeric DNA bindingDNA bindingTelomeric DNASingle Myb-like domainTelomeric DNA-binding proteinMyb-like domainDistinct gene familiesGenome data baseDNA-binding proteinsSearch of genesRegion C-terminalFamily 2Isolated C-terminal fragmentFamily 1Plant telomeresMyb domainArabidopsis genesGene familyC-terminal fragmentImmunoprecipitation experimentsC-terminusRecombinant proteinsC-terminalProtein composition