Mutations in Plasmodium falciparum actin-binding protein coronin confer reduced artemisinin susceptibility
Demas A, Sharma A, Wong W, Early A, Redmond S, Bopp S, Neafsey D, Volkman S, Hartl D, Wirth D. Mutations in Plasmodium falciparum actin-binding protein coronin confer reduced artemisinin susceptibility. Proceedings Of The National Academy Of Sciences Of The United States Of America 2018, 115: 12799-12804. PMID: 30420498, PMCID: PMC6294886, DOI: 10.1073/pnas.1812317115.Peer-Reviewed Original ResearchConceptsCRISPR/Cas9-mediated gene editingDomain protein familyWhole genome sequence analysisProtein familySecond geneFunctional validationMutant formsDifferent genesMolecular mechanismsParental linesSequence analysisType of resistanceGene editingAfrican parasitesArtemisinin susceptibilityMechanisms of resistanceGenesGenetic determinantsParental parasitesMutationsSurvival assaysIndependent selectionParasite clonesProteinActive formDe Novo Mutations Resolve Disease Transmission Pathways in Clonal Malaria
Redmond SN, MacInnis BM, Bopp S, Bei AK, Ndiaye D, Hartl DL, Wirth DF, Volkman SK, Neafsey DE. De Novo Mutations Resolve Disease Transmission Pathways in Clonal Malaria. Molecular Biology And Evolution 2018, 35: 1678-1689. PMID: 29722884, PMCID: PMC5995194, DOI: 10.1093/molbev/msy059.Peer-Reviewed Original ResearchConceptsDe novo mutationsEvolutionary ratesSlow evolutionary rateNovo mutationsComplex life cycleSlow generation timeLow-complexity regionsGenomic regionsLarge genomesGenomic epidemiology approachReintroduction scenariosCombination of sequencingP. falciparumViral speciesMutation rateClonal lineagesGenomeMutation studiesLibrary preparationIdentical parasitesGeneration timeBacterial pathogensMalaria parasitesMutationsGenomic epidemiology