2020
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis
2007
Refractoriness in Tsetse Flies (Diptera: Glossinidae) May be a Matter of Timing
Nayduch D, Aksoy S. Refractoriness in Tsetse Flies (Diptera: Glossinidae) May be a Matter of Timing. Journal Of Medical Entomology 2007, 51 DOI: 10.1603/0022-2585(2007)44[660:ritfdg]2.0.co;2.Peer-Reviewed Original ResearchAttacin expressionTsetse fliesReverse geneticsRNA interferenceFly speciesGene expressionAttacinCertain speciesSusceptible speciesBlood feedingSpeciesVector competenceFliesImmune challengeEfficient vectorDevastating diseaseExpressionRefractory speciesTsetseG. pallidipesAfrican trypanosomiasisHumoral immune responseSalivary glandsRepressionDisease transmissionRefractoriness in Tsetse Flies (Diptera: Glossinidae) May be a Matter of Timing
Nayduch D, Aksoy S. Refractoriness in Tsetse Flies (Diptera: Glossinidae) May be a Matter of Timing. Journal Of Medical Entomology 2007, 44: 660-665. DOI: 10.1603/0022-2585%282007%2944%5b660%3aritfdg%5d2.0.co%3b2.Peer-Reviewed Original ResearchAttacin expressionTsetse fliesReverse geneticsRNA interferenceFly speciesGene expressionAttacinCertain speciesSusceptible speciesBlood feedingSpeciesVector competenceFliesImmune challengeEfficient vectorSusceptible fliesDevastating diseaseExpressionRefractory speciesTsetseG. pallidipesAfrican trypanosomiasisHumoral immune responseSalivary glandsRepression
1998
Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans
Cappello M, Li S, Chen X, Li C, Harrison L, Narashimhan S, Beard C, Aksoy S. Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14290-14295. PMID: 9826693, PMCID: PMC24366, DOI: 10.1073/pnas.95.24.14290.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnticoagulantsAntithrombin ProteinsAntithrombinsBase SequenceBloodCloning, MolecularDigestive SystemDNA, ComplementaryEatingEscherichia coliFemaleGene Expression RegulationInsect ProteinsMaleMolecular Sequence DataPupaRecombinant ProteinsRNA, MessengerSalivary GlandsThrombinTranscription, GeneticTsetse FliesConceptsTsetse thrombin inhibitorPutative secretory signal peptideNative proteinGlossina morsitans morsitansFull-length cDNADegenerate oligonucleotide probeSecretory signal peptideSalivary gland cDNA libraryLack of homologyMorsitans morsitansUntranslated segmentSignal peptideInhibitor geneCDNA librarySingle copyTsetse genomeAdult tsetse fliesFamily of moleculesSegment codesEscherichia coliOptimal inhibitory activityOligonucleotide probesTsetse fliesSalivary glandsGut tissue