2020
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis
2005
Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host
Toh H, Weiss BL, Perkin SA, Yamashita A, Oshima K, Hattori M, Aksoy S. Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host. Genome Research 2005, 16: 149-156. PMID: 16365377, PMCID: PMC1361709, DOI: 10.1101/gr.4106106.Peer-Reviewed Original ResearchConceptsHost developmental stageTemporal expression profilesDifferent temporal expression profilesGenome erosionMutualistic lifestylesGene inventorySymbiont infectionSymbiotic lifestyleHost developmentTranscript abundanceMetabolism of carbohydratesFlagellum structureSodalisComplete sequenceExpression profilesSPI-2SPI-1ChromosomesTsetse hostBacterial speciesVeterinary significancePathogenic microbesDevelopmental stagesPseudogenesTsetse flies
2001
A novel application of gene arrays: Escherichia coli array provides insight into the biology of the obligate endosymbiont of tsetse flies
Akman L, Aksoy S. A novel application of gene arrays: Escherichia coli array provides insight into the biology of the obligate endosymbiont of tsetse flies. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 7546-7551. PMID: 11404467, PMCID: PMC34705, DOI: 10.1073/pnas.131057498.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsDNA MethylationDNA ReplicationDNA, BacterialDNA, ComplementaryEnterobacteriaceaeEscherichia coliGenome, BacterialMolecular Sequence DataNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisProtein BiosynthesisSymbiosisTranscription, GeneticTsetse FliesConceptsObligate endosymbiontsComplete genome sequence dataGene arrayBiosynthesis of cofactorsE. coli genesGenome sequence dataTsetse fliesFacultative anaerobic organismsObligate symbiontsOrthologous genesWigglesworthia glossinidiaSmall genomesGenome sizeSymbiotic associationGenome informationDNA replicationRelated organismsColi geneSymbiotic lifeSequence dataWigglesworthiaGene productsExpression analysisTransport proteinsExpression profiles