2023
Dynamics of eastern equine encephalitis virus during the 2019 outbreak in the Northeast United States
Hill V, Koch R, Bialosuknia S, Ngo K, Zink S, Koetzner C, Maffei J, Dupuis A, Backenson P, Oliver J, Bransfield A, Misencik M, Petruff T, Shepard J, Warren J, Gill M, Baele G, Vogels C, Gallagher G, Burns P, Hentoff A, Smole S, Brown C, Osborne M, Kramer L, Armstrong P, Ciota A, Grubaugh N. Dynamics of eastern equine encephalitis virus during the 2019 outbreak in the Northeast United States. Current Biology 2023, 33: 2515-2527.e6. PMID: 37295427, PMCID: PMC10316540, DOI: 10.1016/j.cub.2023.05.047.Peer-Reviewed Original ResearchGenomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages
Oliveira G, Vogels C, Zolfaghari A, Saraf S, Klitting R, Weger-Lucarelli J, P Leon K, Ontiveros C, Agarwal R, Tsetsarkin K, Harris E, Ebel G, Wohl S, Grubaugh N, Andersen K. Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages. PLOS Neglected Tropical Diseases 2023, 17: e0011055. PMID: 36753510, PMCID: PMC9907835, DOI: 10.1371/journal.pntd.0011055.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsEvolution, MolecularGenomicsHumansMosquito VectorsZika VirusZika Virus InfectionConceptsHuman primary cellsFitness differencesVirus lineagesRapid molecular evolutionPrimary cellsShort generation timeAmino acid sitesFitness changesHigh mutation ratePhenotypic evolutionMolecular evolutionPositive selectionMutation rateLineagesPhenotypic analysisPhenotypic changesRNA virusesGeneration timeRecombinant virusesAedes aegypti mosquitoesReplicative fitnessFitnessAegypti mosquitoesMosquitoesZika virus
2021
Lying in wait: the resurgence of dengue virus after the Zika epidemic in Brazil
Brito AF, Machado LC, Oidtman RJ, Siconelli MJL, Tran QM, Fauver JR, Carvalho RDO, Dezordi FZ, Pereira MR, de Castro-Jorge LA, Minto ECM, Passos LMR, Kalinich CC, Petrone ME, Allen E, España GC, Huang AT, Cummings DAT, Baele G, Franca RFO, da Fonseca BAL, Perkins TA, Wallau GL, Grubaugh ND. Lying in wait: the resurgence of dengue virus after the Zika epidemic in Brazil. Nature Communications 2021, 12: 2619. PMID: 33976183, PMCID: PMC8113494, DOI: 10.1038/s41467-021-22921-7.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAntibodies, ViralBrazilChildChild, PreschoolDengueDengue VirusDisease SusceptibilityEpidemicsEpidemiological MonitoringFemaleGenome, ViralHumansImmunity, HeterologousIncidenceInfantInfant, NewbornMaleMiddle AgedMolecular TypingMosquito VectorsPhylogeographySerotypingYoung AdultZika VirusZika Virus InfectionConceptsDengue virus serotype 1Zika epidemicZika virus epidemicDENV lineagesVirus serotype 1DENV infectionProtective immunityDENV transmissionDengue susceptibilityDengue virusViral spreadLow transmission levelsSerotype 1Virus epidemicMajor outbreaksModel mosquitoEpidemicInfectionDengueTransmission suitabilityDengue incidenceYearsDengue dynamicsOutbreakIncidenceAsynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic
Petrone ME, Earnest R, Lourenço J, Kraemer MUG, Paulino-Ramirez R, Grubaugh ND, Tapia L. Asynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic. Nature Communications 2021, 12: 151. PMID: 33420058, PMCID: PMC7794562, DOI: 10.1038/s41467-020-20391-x.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAedesAnimalsChikungunya FeverChikungunya virusChildChild, PreschoolCommunicable Diseases, EmergingDengueDengue VirusDisease OutbreaksDominican RepublicEndemic DiseasesEpidemiological MonitoringFemaleHumansInfantInfant, NewbornMaleMosquito ControlMosquito VectorsSpatio-Temporal AnalysisYoung AdultZika VirusZika Virus InfectionConceptsDengue outbreakDisease outbreaksDengue fever casesOutbreak of chikungunyaMosquito-Borne VirusesFever casesFuture health crisesMosquito-borne disease outbreaksFuture outbreaksVirus transmissionAedes mosquitoesSustainable disease control measuresHealth crisisVirusDisease control measuresOutbreakChikungunyaConstant surveillanceZikaControl measuresDominican Republic
2020
Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors
Lemos D, Stuart JB, Louie W, Singapuri A, Ramírez AL, Watanabe J, Usachenko J, Keesler RI, Martin CS, Li T, Martyn C, Oliveira G, Saraf S, Grubaugh ND, Andersen KG, Thissen J, Allen J, Borucki M, Tsetsarkin KA, Pletnev AG, Chiu CY, Van Rompay KKA, Coffey LL. Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors. Journal Of Virology 2020, 94: 10.1128/jvi.01605-20. PMID: 32999034, PMCID: PMC7925200, DOI: 10.1128/jvi.01605-20.Peer-Reviewed Original ResearchConceptsCongenital Zika syndromeZika virusFetal infectionFetal deathZika syndromePregnant micePregnant wild-type miceRhesus macaque fetusesPregnant rhesus macaquesZika virus infectionWild-type miceNonpregnant hostsPlacental infectionLow viremiaMajority of animalsPregnant womenPregnant macaquesSevere outcomesMacaque fetusesVirus infectionFetusesInfectionZIKV polyproteinMouse fetusesRhesus macaques
2019
Genomic Epidemiology as a Public Health Tool to Combat Mosquito-Borne Virus Outbreaks
Pollett S, Fauver JR, Berry I, Melendrez M, Morrison A, Gillis LD, Johansson MA, Jarman RG, Grubaugh ND. Genomic Epidemiology as a Public Health Tool to Combat Mosquito-Borne Virus Outbreaks. The Journal Of Infectious Diseases 2019, 221: s308-s318. PMID: 31711190, PMCID: PMC11095994, DOI: 10.1093/infdis/jiz302.Peer-Reviewed Original ResearchConceptsGenomic epidemiologyVirus outbreakPublic health responsePublic health toolPublic health agenciesPublic health emergencyKey epidemiological questionsStructured surveillanceChikungunya virusHealth responseEpidemiologyVirus controlHealth toolsHealth agenciesWest NileHealth emergencyVirus genomic dataYellow feverEpidemiological questionsControl of mosquitoesOutbreakOngoing advancesVirus threatsMosquitoesFeverArbovirus coinfection and co-transmission: A neglected public health concern?
Vogels CBF, Rückert C, Cavany SM, Perkins TA, Ebel GD, Grubaugh ND. Arbovirus coinfection and co-transmission: A neglected public health concern? PLOS Biology 2019, 17: e3000130. PMID: 30668574, PMCID: PMC6358106, DOI: 10.1371/journal.pbio.3000130.Peer-Reviewed Original ResearchConceptsImpact of coinfectionPublic health concernPublic healthEpidemiological synergyVirus coinfectionAedes aegypti mosquitoesClinical diseaseSuch coinfectionsZika virusCoinfectionOutbreaks of virusesHealth concernAegypti mosquitoesMultiple virusesTransmission dynamicsVirusHealthHumansMosquitoesDiseaseDengue
2017
Genomic epidemiology reveals multiple introductions of Zika virus into the United States
Grubaugh ND, Ladner JT, Kraemer MUG, Dudas G, Tan AL, Gangavarapu K, Wiley MR, White S, Thézé J, Magnani DM, Prieto K, Reyes D, Bingham AM, Paul LM, Robles-Sikisaka R, Oliveira G, Pronty D, Barcellona CM, Metsky HC, Baniecki ML, Barnes KG, Chak B, Freije CA, Gladden-Young A, Gnirke A, Luo C, MacInnis B, Matranga CB, Park DJ, Qu J, Schaffner SF, Tomkins-Tinch C, West KL, Winnicki SM, Wohl S, Yozwiak NL, Quick J, Fauver JR, Khan K, Brent SE, Reiner RC, Lichtenberger PN, Ricciardi MJ, Bailey VK, Watkins DI, Cone MR, Kopp EW, Hogan KN, Cannons AC, Jean R, Monaghan AJ, Garry RF, Loman NJ, Faria NR, Porcelli MC, Vasquez C, Nagle ER, Cummings DAT, Stanek D, Rambaut A, Sanchez-Lockhart M, Sabeti PC, Gillis LD, Michael SF, Bedford T, Pybus OG, Isern S, Palacios G, Andersen KG. Genomic epidemiology reveals multiple introductions of Zika virus into the United States. Nature 2017, 546: 401-405. PMID: 28538723, PMCID: PMC5536180, DOI: 10.1038/nature22400.Peer-Reviewed Original ResearchZika virus evolution and spread in the Americas
Metsky HC, Matranga CB, Wohl S, Schaffner SF, Freije CA, Winnicki SM, West K, Qu J, Baniecki ML, Gladden-Young A, Lin AE, Tomkins-Tinch CH, Ye SH, Park DJ, Luo CY, Barnes KG, Shah RR, Chak B, Barbosa-Lima G, Delatorre E, Vieira YR, Paul LM, Tan AL, Barcellona CM, Porcelli MC, Vasquez C, Cannons AC, Cone MR, Hogan KN, Kopp EW, Anzinger JJ, Garcia KF, Parham LA, Ramírez RMG, Montoya MCM, Rojas DP, Brown CM, Hennigan S, Sabina B, Scotland S, Gangavarapu K, Grubaugh ND, Oliveira G, Robles-Sikisaka R, Rambaut A, Gehrke L, Smole S, Halloran ME, Villar L, Mattar S, Lorenzana I, Cerbino-Neto J, Valim C, Degrave W, Bozza PT, Gnirke A, Andersen KG, Isern S, Michael SF, Bozza FA, Souza TML, Bosch I, Yozwiak NL, MacInnis BL, Sabeti PC. Zika virus evolution and spread in the Americas. Nature 2017, 546: 411-415. PMID: 28538734, PMCID: PMC5563848, DOI: 10.1038/nature22402.Peer-Reviewed Original Research
2016
Genetic Drift during Systemic Arbovirus Infection of Mosquito Vectors Leads to Decreased Relative Fitness during Host Switching
Grubaugh ND, Weger-Lucarelli J, Murrieta RA, Fauver JR, Garcia-Luna SM, Prasad AN, Black WC, Ebel GD. Genetic Drift during Systemic Arbovirus Infection of Mosquito Vectors Leads to Decreased Relative Fitness during Host Switching. Cell Host & Microbe 2016, 19: 481-492. PMID: 27049584, PMCID: PMC4833525, DOI: 10.1016/j.chom.2016.03.002.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsArbovirusesCulexGenetic DriftGenetic VariationMosquito VectorsMutationSpecies SpecificityWest Nile virusConceptsRelative fitnessMosquito speciesLower relative fitnessWest Nile virusComplex virus populationsSignificant fitness costMosquito-borne RNA virusesHost switchingGenetic driftGenetic diversityAdaptive potentialPopulation expansionFitness costsAvian cellsDeleterious mutationsMutational diversityRNA virusesBridge vectorsViral populationsVirus populationsMosquito vectorsFitnessSpeciesDiversityNile virus