1993
Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL
Bruck R, Benedetti A, Strazzabosco M, Boyer J. Intracellular alkalinization stimulates bile flow and vesicular-mediated exocytosis in IPRL. American Journal Of Physiology 1993, 265: g347-g353. PMID: 8368316, DOI: 10.1152/ajpgi.1993.265.2.g347.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAlkaliesAnimalsAnionsBariumBicarbonatesBileBiological TransportColchicineExocytosisHorseradish PeroxidaseHydrogen-Ion ConcentrationIn Vitro TechniquesIntracellular MembranesLiverMalePotassium ChannelsRatsRats, Sprague-DawleyConceptsBiliary HCO3- excretionBile flowHCO3- excretionIntracellular alkalinizationHCO3- exchangerAcute alkaline loadVesicle-mediated exocytosisBiliary excretionTransient increasePH recoveryAlkaline loadMembrane depolarizationRat liverChannel blocker BaCl2Intact liverLiverAlkalinizationMM NH4ClHCO3- concentrationDisulfonic acidDIDSRat hepatocytesColchicinePretreatmentExocytosis
1984
Isolated rat hepatocyte couplets: a primary secretory unit for electrophysiologic studies of bile secretory function.
Graf J, Gautam A, Boyer J. Isolated rat hepatocyte couplets: a primary secretory unit for electrophysiologic studies of bile secretory function. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 6516-6520. PMID: 6149546, PMCID: PMC391955, DOI: 10.1073/pnas.81.20.6516.Peer-Reviewed Original ResearchConceptsBile secretory functionElectrophysiologic studySecretory functionHepatocyte coupletsOrganic anion fluoresceinSteady-state intracellularCanalicular lumenCanalicular vacuoleInput resistanceRat liverCollagenase perfusionIntact liverLiverSecretionParacellular barrierBile canalicular lumenSecretory unitsLumenCellsJunctional pathwayElectrical driving forcePerfusion