Generation of transgenic Hydra by embryo microinjection.
Juliano CE, Lin H, Steele RE. Generation of transgenic Hydra by embryo microinjection. Journal Of Visualized Experiments 2014, 51888. PMID: 25285460, PMCID: PMC4828061, DOI: 10.3791/51888.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedDNAFemaleGene Knockdown TechniquesHydraMaleMicroinjectionsPlasmidsConceptsTransgenic HydraFundamental biological processesEarly stage embryosPatches of tissuePure cell populationsMulticellular animalsStudy of regenerationGene functionMolecular functionsPhylum CnidariaParticular lineageTransgenic linesGene perturbationsSister groupTransgenic tissuesTransgenic methodsPromoter functionAsexual propagationBiological processesEmbryo microinjectionStage embryosLive cellsStem cellsHatchlingsBiochemical analysis