2014
PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells
Cheng EC, Kang D, Wang Z, Lin H. PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells. PLOS ONE 2014, 9: e97821. PMID: 25238487, PMCID: PMC4169525, DOI: 10.1371/journal.pone.0097821.Peer-Reviewed Original ResearchConceptsPIWI proteinsEmbryonic stem cellsInduced pluripotent stem cellsPluripotent stem cellsIPS cellsPiwi genesGermline stem cell maintenanceStem cellsControl iPS cellsDifferentiated somatic cellsStem cell maintenancePIWI protein familyReprogramming of fibroblastsExpression profiling revealsGermline developmentProtein familyKnockout embryosCell maintenanceFemale fertileSomatic cellsEmbryonic fibroblastsDirect reprogrammingProfiling revealsTeratoma assayGerm layers
2013
PIWI proteins are essential for early Drosophila embryogenesis
Mani SR, Megosh H, Lin H. PIWI proteins are essential for early Drosophila embryogenesis. Developmental Biology 2013, 385: 340-349. PMID: 24184635, PMCID: PMC3915879, DOI: 10.1016/j.ydbio.2013.10.017.Peer-Reviewed Original ResearchConceptsPIWI proteinsMitotic defectsEarly embryogenesisArgonaute/PIWI protein familySmall non-coding RNAsEarly Drosophila embryogenesisAsynchronous nuclear divisionPosttranscriptional gene regulationSevere mitotic defectsPIWI protein familyNon-coding RNAsCell cycle progressionCell cycle arrestGermline developmentDrosophila embryogenesisChromatin structureGene regulationSyncytial embryosMitotic machinerySomatic nucleiProtein familyPiwiNuclear divisionNuclear migrationEssential functionsPiwi Genes Are Dispensable for Normal Hematopoiesis in Mice
Nolde MJ, Cheng EC, Guo S, Lin H. Piwi Genes Are Dispensable for Normal Hematopoiesis in Mice. PLOS ONE 2013, 8: e71950. PMID: 24058407, PMCID: PMC3751959, DOI: 10.1371/journal.pone.0071950.Peer-Reviewed Original ResearchConceptsPiwi genesHematopoietic stem cellsNormal adult hematopoiesisPIWI protein familyStem cellsStem/progenitor cellsDiverse organismsAdult hematopoiesisProtein familyLong-term hematopoiesisMyeloablative stressCompetitive transplantationTransient expressionHuman leukemia cell linesHSC compartmentLeukemia cell linesGenesProliferative stateNormal hematopoiesisCell typesMIWI2Progenitor cellsLineage reconstitutionHematopoiesisCell proliferation
2008
MILI, a PIWI-interacting RNA-binding Protein, Is Required for Germ Line Stem Cell Self-renewal and Appears to Positively Regulate Translation*
Unhavaithaya Y, Hao Y, Beyret E, Yin H, Kuramochi-Miyagawa S, Nakano T, Lin H. MILI, a PIWI-interacting RNA-binding Protein, Is Required for Germ Line Stem Cell Self-renewal and Appears to Positively Regulate Translation*. Journal Of Biological Chemistry 2008, 284: 6507-6519. PMID: 19114715, PMCID: PMC2649106, DOI: 10.1074/jbc.m809104200.Peer-Reviewed Original ResearchConceptsGerm line stem cellsStem cellsArgonaute/PIWI protein familyMicro-RNA pathwayGerm line developmentMammalian stem cellsPIWI-interacting RNAsCap-binding complexRNA-binding proteinPIWI protein familyCellular mRNA levelsStem cell populationSelf-renewing divisionsArgonaute functionsPIWI proteinsRegulates TranslationPIWI subfamiliesEpigenetic regulationProtein familyCell maintenanceRNA interferenceChromatoid bodyMILIMolecular mechanismsEarly spermatocytes