2002
On the natriuretic effect of verapamil: inhibition of ENaC and transepithelial sodium transport
Segal AS, Hayslett JP, Desir GV. On the natriuretic effect of verapamil: inhibition of ENaC and transepithelial sodium transport. American Journal Of Physiology. Renal Physiology 2002, 283: f765-f770. PMID: 12217868, DOI: 10.1152/ajprenal.00253.2001.Peer-Reviewed Original ResearchMeSH KeywordsAldosteroneAnimalsBiological Transport, ActiveCalcium Channel BlockersCells, CulturedDose-Response Relationship, DrugElectrophysiologyEpithelial Sodium ChannelsEpitheliumIn Vitro TechniquesInsulinKidneyKidney Tubules, CollectingNatriuresisNifedipineOocytesPatch-Clamp TechniquesSodiumSodium Channel BlockersSodium ChannelsVasopressinsVerapamilXenopus laevisConceptsNatriuretic effectDirect tubular effectAdministration of verapamilRemoval of extracellularTubular effectsTransepithelial sodium transportHemodynamic changesInhibition of ENaCSurrogate markerChannel blockersMicroM verapamilDistal tubulesA6 cellsMicroM amilorideUssing chambersVerapamilL-typeSodium transportBasolateral sideTransepithelial resistanceSignificant increaseApical sidePermeable supportsInhibitionTransepithelial
1993
Identification of a novel K-channel gene (KC22) that is highly expressed in distal tubule of rabbit kidney
Desir GV, Velazquez H. Identification of a novel K-channel gene (KC22) that is highly expressed in distal tubule of rabbit kidney. American Journal Of Physiology 1993, 264: f128-f133. PMID: 7679255, DOI: 10.1152/ajprenal.1993.264.1.f128.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionRabbit kidneyKidney cortexWhole kidney cortexShaker gene familyDistal tubule cellsVoltage-gated K channelsRenal tissueDistal tubulesTubule cellsRibonuclease protectionPrimary culturesKidneyChain reactionExpression levelsK channelsCortexTubulesPartial-length sequencesAbundant isoformIsoformsKidney cDNAK channel genes