2011
An unusual tRNAThr derived from tRNAHis reassigns in yeast mitochondria the CUN codons to threonine
Su D, Lieberman A, Lang BF, Simonović M, Söll D, Ling J. An unusual tRNAThr derived from tRNAHis reassigns in yeast mitochondria the CUN codons to threonine. Nucleic Acids Research 2011, 39: 4866-4874. PMID: 21321019, PMCID: PMC3113583, DOI: 10.1093/nar/gkr073.Peer-Reviewed Original ResearchConceptsCUN codonsYeast mitochondriaGenetic codeAlloacceptor tRNA gene recruitmentComprehensive phylogenetic analysisStandard genetic codeThreonyl-tRNA synthetaseHistidyl-tRNA synthetaseGene recruitmentEvolutionary originPhylogenetic analysisRecoding eventBiochemical experimentsFirst nucleotideAnticodon loopMST1CodonFirst clear exampleYeastMitochondriaThreonineSynthetaseCandida albicansGenomeClear example
1991
Histidine tRNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and physical properties.
Pande S, Jahn D, Söll D. Histidine tRNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and physical properties. Journal Of Biological Chemistry 1991, 266: 22826-22831. PMID: 1660461, DOI: 10.1016/s0021-9258(18)54428-8.Peer-Reviewed Original ResearchConceptsAdditional nucleotidesHistidine tRNA genesPolymin P precipitationTRNA genesSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisTRNA speciesSulfate-polyacrylamide gel electrophoresisRate zonal sedimentationHomodimeric structureGuanylyltransferaseRelative molecular weightTRNAATP-agaroseGel filtrationAbolishes activityHistidine tRNANative enzymeGuanosine residuesAcceptor RNAEnzymatic activityUnfractionated tRNAGuanosine substrateZonal sedimentationGel electrophoresis
1990
Enzymatic addition of guanylate to histidine transfer RNA
Williams J, Cooley L, Söll D. Enzymatic addition of guanylate to histidine transfer RNA. Methods In Enzymology 1990, 181: 451-462. PMID: 2166216, DOI: 10.1016/0076-6879(90)81143-i.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChromatography, AffinityChromatography, DEAE-CelluloseChromatography, Ion ExchangeDrosophilaElectrophoresis, Polyacrylamide GelGuanosine TriphosphateKineticsNucleotidyltransferasesPhosphorus RadioisotopesRadioisotope Dilution TechniqueRNA, Transfer, Amino Acid-SpecificRNA, Transfer, HisSaccharomyces cerevisiaeSubstrate SpecificityConceptsHistidine tRNATransfer RNABacteriophage T5Yeast enzymeEnzyme migratesUridine residuesExtra nucleotidesLigase mechanismAdditional nucleotidesEnzymatic additionGel filtration chromatographyEnzyme intermediateTRNAAbsolute requirementEnzymeMolecular weightNucleotidesUltrogel AcA 34Filtration chromatographyATPDrosophilaAcA 34Molecular weight markersYeastTitration experiments
1988
The 5′-terminal guanylate of chloroplast histidine tRNA is encoded in its gene.
Burkard U, Söll D. The 5′-terminal guanylate of chloroplast histidine tRNA is encoded in its gene. Journal Of Biological Chemistry 1988, 263: 9578-9581. PMID: 2838471, DOI: 10.1016/s0021-9258(19)81555-7.Peer-Reviewed Original ResearchAnimalsBase SequenceChloroplastsCyanobacteriaDNA, RecombinantEndoribonucleasesEscherichia coliEscherichia coli ProteinsEuglena gracilisGuanine NucleotidesGuanosine MonophosphateMolecular Sequence DataPentosyltransferasesPlantsRibonuclease PRNA PrecursorsRNA, Transfer, Amino Acid-SpecificRNA, Transfer, HisSaccharomyces cerevisiaeVegetablesProcessing of histidine transfer RNA precursors. Abnormal cleavage site for RNase P.
Burkard U, Willis I, Söll D. Processing of histidine transfer RNA precursors. Abnormal cleavage site for RNase P. Journal Of Biological Chemistry 1988, 263: 2447-2451. PMID: 3276688, DOI: 10.1016/s0021-9258(18)69227-0.Peer-Reviewed Original Research