1996
Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms
Hodgkinson V, Soong L, Duboise S, McMahon-Pratt D. Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms. Experimental Parasitology 1996, 83: 94-105. PMID: 8654556, DOI: 10.1006/expr.1996.0053.Peer-Reviewed Original ResearchConceptsAmastigote-like formsNorthern blot analysisDevelopmental biologyContinual cultureAxenic cultureMolecular biologyTransformation of promastigotesOrganismsDNA probesGeneration timeCultured amastigotesAxenic cultivationBlot analysisExpression levelsSpecies of LeishmaniaSpeciesProtein reactiveBiologyDegrees CFirst reportPeriod of growthAmastigotesPromastigotesLeishmania amazonensisCultivation
1990
Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis.
Lohman K, Langer P, McMahon-Pratt D. Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 8393-8397. PMID: 2236047, PMCID: PMC54962, DOI: 10.1073/pnas.87.21.8393.Peer-Reviewed Original ResearchConceptsGP46/MDNA sequence dataHydrophobic leader peptideCarboxyl-terminal domainMetal-binding motifGlycosyl-phosphatidylinositol anchorAmino-terminal portionProtozoan Leishmania amazonensisSouthern blot analysisGene familyMature proteinPutative attachment siteMolecular cloningProtein comprisesRepetitive sequencesSequence dataCarboxyl terminusCysteine residuesProtein sequencesLeader peptideMembrane locationBinding motifLeishmania amazonensisMembrane glycoproteinsBlot analysis