2000
The Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*
Kar S, Soong L, Colmenares M, Goldsmith-Pestana K, McMahon-Pratt D. The Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*. Journal Of Biological Chemistry 2000, 275: 37789-37797. PMID: 10969068, DOI: 10.1074/jbc.m002149200.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumSpecific nuclease activityBlot analysisImmunofluorescence microscopic analysisIntracellular amastigote stageProtein sequence dataSingle-strand nucleasePeripheral blood mononuclear cellsSouthern blot analysisNorthern blot analysisBlood mononuclear cellsMembrane proteinsRNA stabilitySubcellular localizationFuture vaccine studiesDNA repairSequence dataTerminal domainAmerican cutaneous leishmaniasisSequence analysisNuclease activityAmastigote stagePromastigote stageSpecific nucleasesT helper
1994
The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases
Duboise S, Vannier-Santos M, Costa-Pinto D, Rivas L, Pan A, Traub-Cseko Y, De Souza W, McMahon-Pratt D. The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases. Molecular And Biochemical Parasitology 1994, 68: 119-132. PMID: 7891736, DOI: 10.1016/0166-6851(94)00157-x.Peer-Reviewed Original ResearchConceptsTerminal domainMature proteinaseFlagellar pocketCysteine proteinasesParasite cell divisionCOOH-terminal domainProtein sequence dataAmastigote cysteine proteinasesElectron microscopic immunolocalizationLow-level secretionCell divisionSequence dataPlasma membraneIntracellular targetingLeishmania pifanoiProteolytic processingPulse labelingAcid proteinase inhibitorsProteinase inhibitorsCultured amastigotesLevel secretionL. pifanoiExtension domainBiosynthesisEnzymatic processing
1993
Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction
Traub-Cseko Y, Duboise M, Boukai L, McMahon-Pratt D. Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction. Molecular And Biochemical Parasitology 1993, 57: 101-115. PMID: 8426606, DOI: 10.1016/0166-6851(93)90248-v.Peer-Reviewed Original ResearchConceptsC-terminal extensionCysteine proteinase geneComparative sequence analysisCysteine proteinasesActive site residuesAmastigote cysteine proteinasesMexicana complexChromosome locationGenomic arrangementDistinct genesCDNA sequenceSequence dataDNA sequencesTrypanosoma bruceiProtein sequencesBiological functionsProteinase geneSite residuesDegenerate primersSelective conservationLysosomal organellesGlycine residueGenomic copiesSequence analysisLeishmania mexicana complex
1990
Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis.
Lohman K, Langer P, McMahon-Pratt D. Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 8393-8397. PMID: 2236047, PMCID: PMC54962, DOI: 10.1073/pnas.87.21.8393.Peer-Reviewed Original ResearchConceptsGP46/MDNA sequence dataHydrophobic leader peptideCarboxyl-terminal domainMetal-binding motifGlycosyl-phosphatidylinositol anchorAmino-terminal portionProtozoan Leishmania amazonensisSouthern blot analysisGene familyMature proteinPutative attachment siteMolecular cloningProtein comprisesRepetitive sequencesSequence dataCarboxyl terminusCysteine residuesProtein sequencesLeader peptideMembrane locationBinding motifLeishmania amazonensisMembrane glycoproteinsBlot analysis
1989
Amastigote and epimastigote stage-specific components of Trypanosoma cruzi characterized by using monoclonal antibodies. Purification and molecular characterization of an 83-kilodalton amastigote protein.
Pan A, McMahon-Pratt D. Amastigote and epimastigote stage-specific components of Trypanosoma cruzi characterized by using monoclonal antibodies. Purification and molecular characterization of an 83-kilodalton amastigote protein. The Journal Of Immunology 1989, 143: 1001-8. PMID: 2501384, DOI: 10.4049/jimmunol.143.3.1001.Peer-Reviewed Original ResearchConceptsTissue culture-derived trypomastigotesTrypanosoma cruziCruzi amastigotesPreliminary sequence dataStage-specific componentsCulture-derived trypomastigotesStage-specific monoclonal antibodiesSingle protein moleculesMembrane-enriched preparationsAmino acid compositionSequence dataEpimastigote membranesHuman chagasic seraStage differentiationAxenic cultureMolecular characterizationT. cruzi amastigotesEpimastigotesProtein moleculesProteinChagas diseaseMonoclonal antibodiesLeishmania speciesAcid compositionChagasic sera