2015
Regulation of actin polymerization by tropomodulin-3 controls megakaryocyte actin organization and platelet biogenesis
Sui Z, Nowak RB, Sanada C, Halene S, Krause DS, Fowler VM. Regulation of actin polymerization by tropomodulin-3 controls megakaryocyte actin organization and platelet biogenesis. Blood 2015, 126: 520-530. PMID: 25964668, PMCID: PMC4513252, DOI: 10.1182/blood-2014-09-601484.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsApoptosisBlood PlateletsBlotting, WesternCell MembraneCell ProliferationCells, CulturedCytoplasmEmbryo, MammalianFemaleFluorescent Antibody TechniqueHematopoiesisHemorrhageImmunoprecipitationMegakaryocytesMiceMice, KnockoutMicroscopy, ConfocalMicroscopy, Electron, TransmissionMicroscopy, FluorescencePloidiesPolymerizationTropomodulinConceptsPlatelet biogenesisDemarcation membrane systemF-actinTropomodulin-3Organelle distributionProplatelet formationActin polymerizationF-actin cappingF-actin organizationF-actin cytoskeletonWild-type megakaryocytesActin cytoskeletonActin organizationMK differentiationTmod isoformsLarge proplateletsBiogenesisContractile bundlesActin filamentsDMS formationBinds tropomyosinBud sizeMK numberConfocal microscopyCytoskeleton
2013
Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells
Lawton BR, Sosa JA, Roman S, Krause DS. Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells. Stem Cell Reviews And Reports 2013, 9: 578-585. PMID: 23719997, DOI: 10.1007/s12015-013-9447-2.Peer-Reviewed Original ResearchMeSH KeywordsCell Culture TechniquesCell DifferentiationCell LineCell LineageCell MovementCell SurvivalCollagenDrug CombinationsEmbryonic Stem CellsEndodermFluorescent Antibody TechniqueGene Expression Regulation, DevelopmentalGlutamine-Fructose-6-Phosphate Transaminase (Isomerizing)Hepatocyte Nuclear Factor 3-alphaHepatocyte Nuclear Factor 3-betaHumansLamininProteoglycansReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionSOXF Transcription FactorsTime FactorsConceptsHuman embryonic stem cellsEmbryonic stem cellsDefinitive endodermEndodermal differentiationGene expression patternsStem cellsPrecardiac mesodermExpression patternsLow-serum mediumCell deathMesenchymal transitionMigratory characteristicsKey eventsEndodermDifferentiationCell viabilityActivin ASerum mediumCellsGastrulationImproved protocolMesodermInductionGenesNutrients
2008
Influence of Culture Medium on Smooth Muscle Cell Differentiation from Human Bone Marrow–Derived Mesenchymal Stem Cells
Gong Z, Calkins G, Cheng EC, Krause D, Niklason LE. Influence of Culture Medium on Smooth Muscle Cell Differentiation from Human Bone Marrow–Derived Mesenchymal Stem Cells. Tissue Engineering Part A 2008, 15: 319-330. PMID: 19115826, PMCID: PMC2716410, DOI: 10.1089/ten.tea.2008.0161.Peer-Reviewed Original ResearchPhysiological variations of stem cell factor and stromal‐derived factor‐1 in murine models of liver injury and regeneration
Swenson ES, Kuwahara R, Krause DS, Theise ND. Physiological variations of stem cell factor and stromal‐derived factor‐1 in murine models of liver injury and regeneration. Liver International 2008, 28: 308-318. PMID: 18290773, PMCID: PMC2846401, DOI: 10.1111/j.1478-3231.2007.01659.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ProliferationChemokine CXCL12Enzyme-Linked Immunosorbent AssayFluorescent Antibody TechniqueLiverLiver RegenerationMiceRadiographyStem Cell FactorStem CellsTyrosineConceptsStromal-derived factor-1Oval cell proliferationLiver injuryLiver irradiationBile ductCell proliferationSDF-1 levelsArterial smooth muscleFactor 1Cell factorMarrow-derived progenitorsNormal mouse liverPlasma levelsBACKGROUND/Murine modelStem cell factorKupffer cellsSmooth muscleInjuryRegenerative responseOval cellsDihydrocollidineMouse liverMiceLiver progenitors
2007
Limitations of Green Fluorescent Protein as a Cell Lineage Marker
Swenson ES, Price JG, Brazelton T, Krause DS. Limitations of Green Fluorescent Protein as a Cell Lineage Marker. Stem Cells 2007, 25: 2593-2600. PMID: 17615263, DOI: 10.1634/stemcells.2007-0241.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood CellsCattleCell LineageChickensCytomegalovirusFemaleFluorescent Antibody TechniqueGene ExpressionGenes, ReporterGenes, SyntheticGreen Fluorescent ProteinsHumansImmunoenzyme TechniquesMaleMiceMice, Inbred C57BLOrgan SpecificityRegulatory Sequences, Nucleic AcidTissue DistributionTransgenesVisceraConceptsSmall intestineMouse strainsPeripheral blood cellsTransgenic mouse strainReporter mouse strainPrimary rabbit antibodiesDonor originHuman ubiquitin C promoterImmunohistochemical stainingSolid organsCell lineage markersCell lineagesUBC-GFP miceUbiquitin C promoterChicken beta-actinFlow cytometryBlood cellsEnhanced green fluorescent protein (EGFP) reporterMiceOne-tissueAdult liverTissue sectionsIntestineLineage markersRabbit antibodies
2006
Engraftment of Donor‐Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice
Bruscia EM, Ziegler EC, Price JE, Weiner S, Egan ME, Krause DS. Engraftment of Donor‐Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice. Stem Cells 2006, 24: 2299-2308. PMID: 16794262, DOI: 10.1634/stemcells.2006-0166.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBone Marrow TransplantationCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsFemaleFluorescent Antibody TechniqueHematopoietic Stem Cell TransplantationIn Situ Hybridization, FluorescenceMaleMiceMice, Inbred C57BLMice, Inbred StrainsMice, TransgenicRNA, MessengerY ChromosomeConceptsBone marrow-derived cellsMarrow-derived epithelial cellsBone marrow transplantationNewborn miceEpithelial cellsMarrow transplantationGI tractBone marrow-derived epithelial cellsDonor-derived epithelial cellsDoses of busulfanMarrow-derived cellsEngraftment of donorIrradiated adult recipientsMyeloablative regimenPreparative regimenAdult recipientsDifferent regimensEngrafted miceHematopoietic engraftmentGastrointestinal tractSurvival advantageTherapeutic benefitAdult miceMultiple organsBone marrow