2021
Bone Marrow-Derived VSELs Engraft as Lung Epithelial Progenitor Cells after Bleomycin-Induced Lung Injury
Ciechanowicz AK, Sielatycka K, Cymer M, Skoda M, Suszyńska M, Bujko K, Ratajczak MZ, Krause DS, Kucia M. Bone Marrow-Derived VSELs Engraft as Lung Epithelial Progenitor Cells after Bleomycin-Induced Lung Injury. Cells 2021, 10: 1570. PMID: 34206516, PMCID: PMC8303224, DOI: 10.3390/cells10071570.Peer-Reviewed Original ResearchConceptsBronchioalveolar stem cellsOrganoid assaysAT2 cellsStem cellsH2B-GFP fusion proteinLung epithelial progenitor cellsProgenitor cellsEmbryonic-like stem cellsSurfactant protein CSmall embryonic-like stem cellsEpithelial progenitor cellsLung injuryNonhematopoietic stem cellsFusion proteinAlveolar type 2 cellsPhysiological potentialProgenitor activityBleomycin-Induced Lung InjuryH2B-GFP miceWT recipient miceRegenerative functionSPC promoterType 2 cellsVSELsReporter mice
2013
Very Small Embryonic‐Like Stem Cells from the Murine Bone Marrow Differentiate into Epithelial Cells of the Lung
Kassmer SH, Jin H, Zhang PX, Bruscia EM, Heydari K, Lee JH, Kim CF, Kassmer SH, Krause DS. Very Small Embryonic‐Like Stem Cells from the Murine Bone Marrow Differentiate into Epithelial Cells of the Lung. Stem Cells 2013, 31: 2759-2766. PMID: 23681901, PMCID: PMC4536826, DOI: 10.1002/stem.1413.Peer-Reviewed Original ResearchConceptsEpithelial cellsSmall embryonic-like stem cellsLung epithelial cellsEmbryonic-like stem cellsStem/progenitor cellsStem cellsDonor miceHematopoietic stem/progenitor cellsBM cellsAdult BMBone marrowSmall embryonicNonhematopoietic cellsProgenitor cellsBroad differentiation potentialVSELsEngraftmentLungHigh rateNumerous reportsAdult stem cellsDifferentiation potentialCellsFirst reportReport
2012
Nonhematopoietic Cells are the Primary Source of Bone Marrow‐Derived Lung Epithelial Cells
Kassmer SH, Bruscia EM, Zhang P, Krause DS. Nonhematopoietic Cells are the Primary Source of Bone Marrow‐Derived Lung Epithelial Cells. Stem Cells 2012, 30: 491-499. PMID: 22162244, PMCID: PMC3725285, DOI: 10.1002/stem.1003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsBone Marrow CellsBone Marrow TransplantationCell SeparationEpithelial CellsGene ExpressionLuminescent ProteinsLungMiceMice, 129 StrainMice, Inbred C57BLMice, KnockoutMicroscopy, ConfocalPulmonary Surfactant-Associated Protein CRecombinant ProteinsSingle-Cell AnalysisConceptsLung epithelial cellsNonhematopoietic cellsBM cellsEpithelial cellsBone marrowLungs of miceType 2 pneumocytesNonhematopoietic stem cellsNonhematopoietic fractionAdult BMPrimitive stem cell populationNull miceProgenitor cellsMiceStem cell populationCell populationsMarrowStem cellsMultiple tissuesHematopoietic stemBMCellsPrevious studiesEngraftmentLung
2011
Enhanced growth and hepatic differentiation of fetal liver epithelial cells through combinational and temporal adjustment of soluble factors
Qian L, Krause DS, Saltzman WM. Enhanced growth and hepatic differentiation of fetal liver epithelial cells through combinational and temporal adjustment of soluble factors. Biotechnology Journal 2011, 7: 440-448. PMID: 21922669, PMCID: PMC3532892, DOI: 10.1002/biot.201100184.Peer-Reviewed Original Research
2010
Detection of bone marrow–derived lung epithelial cells
Kassmer SH, Krause DS. Detection of bone marrow–derived lung epithelial cells. Experimental Hematology 2010, 38: 564-573. PMID: 20447442, PMCID: PMC2909593, DOI: 10.1016/j.exphem.2010.04.011.Peer-Reviewed Original ResearchMeSH KeywordsBone Marrow CellsBone Marrow TransplantationCell DifferentiationCell SeparationEpithelial CellsHumansLungConceptsMarrow-derived epithelial cellsBone marrow-derived cellsMarrow-derived cellsLung epithelial cellsEpithelial cellsBone marrow-derived epithelial cellsDonor bone marrow originBone marrow originBone marrow cellsBlood cell markersNormal tissue repairSpecific cell subsetsCell subsetsGastrointestinal tractTherapeutic benefitMarrow originMultiple organsEpithelial markersDefinitive dataCell markersMarrow cellsProtein expression patternsTissue repairEngraftmentLung
2008
Bone Marrow–derived Lung Epithelial Cells
Krause DS. Bone Marrow–derived Lung Epithelial Cells. Annals Of The American Thoracic Society 2008, 5: 699-702. PMID: 18684720, PMCID: PMC2645262, DOI: 10.1513/pats.200803-031aw.Peer-Reviewed Original ResearchConceptsMarrow-derived epithelial cellsEpithelial cellsBone marrow-derived cellsMarrow-derived cellsLung epithelial cellsClinical significanceEpithelial-specific genesMultiple organsAdult lungMarrow cellsFurther studiesMultiple mechanismsRecent dataCellsRecent studiesSignificant skepticismEngraftmentLungBone Marrow–derived Cells and Stem Cells in Lung Repair
Krause DS. Bone Marrow–derived Cells and Stem Cells in Lung Repair. Annals Of The American Thoracic Society 2008, 5: 323-327. PMID: 18403327, PMCID: PMC2645242, DOI: 10.1513/pats.200712-169dr.Peer-Reviewed Original ResearchConceptsMarrow-derived epithelial cellsEpithelial cellsBM cellsTissue injuryBone marrow-derived cellsBM-derived cellsMarrow-derived cellsPotential clinical utilityBronchiolar epithelial cellsType II pneumocytesLung damageTracheal epithelial cellsLung repairClinical utilityGI tractBone marrowTissue damagePeer-reviewed studiesNonhematopoietic cell typesBeneficial effectsPotential mechanismsTissue repairLungInjuryTissue microenvironment
2006
Engraftment of Marrow-derived Epithelial Cells
Herzog EL, Krause DS. Engraftment of Marrow-derived Epithelial Cells. Annals Of The American Thoracic Society 2006, 3: 691-695. PMID: 17065375, PMCID: PMC2647654, DOI: 10.1513/pats.200605-109sf.BooksEngraftment of Donor‐Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice
Bruscia EM, Ziegler EC, Price JE, Weiner S, Egan ME, Krause DS. Engraftment of Donor‐Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice. Stem Cells 2006, 24: 2299-2308. PMID: 16794262, DOI: 10.1634/stemcells.2006-0166.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBone Marrow TransplantationCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsFemaleFluorescent Antibody TechniqueHematopoietic Stem Cell TransplantationIn Situ Hybridization, FluorescenceMaleMiceMice, Inbred C57BLMice, Inbred StrainsMice, TransgenicRNA, MessengerY ChromosomeConceptsBone marrow-derived cellsMarrow-derived epithelial cellsBone marrow transplantationNewborn miceEpithelial cellsMarrow transplantationGI tractBone marrow-derived epithelial cellsDonor-derived epithelial cellsDoses of busulfanMarrow-derived cellsEngraftment of donorIrradiated adult recipientsMyeloablative regimenPreparative regimenAdult recipientsDifferent regimensEngrafted miceHematopoietic engraftmentGastrointestinal tractSurvival advantageTherapeutic benefitAdult miceMultiple organsBone marrowThreshold of Lung Injury Required for the Appearance of Marrow‐Derived Lung Epithelia
Herzog EL, Van Arnam J, Hu B, Krause DS. Threshold of Lung Injury Required for the Appearance of Marrow‐Derived Lung Epithelia. Stem Cells 2006, 24: 1986-1992. PMID: 16868209, DOI: 10.1634/stemcells.2005-0579.Peer-Reviewed Original ResearchConceptsBone marrow-derived cellsBone marrow transplantationLung injuryMarrow transplantationLung epitheliumEngraftment of BMDCsLocal host factorsSex-mismatched bone marrow transplantationMarrow-derived cellsType II pneumocytesMyeloablative radiationLung damageHematopoietic chimerismEpithelial chimerismApparent injuryInjuryTransplantationHost factorsEpitheliumEpithelial cellsEpithelial phenotypeLungChimerismPneumocytesPhenotypic changes
2005
Bone marrow plasticity revisited: protection or differentiation in the kidney tubule?
Krause D, Cantley LG. Bone marrow plasticity revisited: protection or differentiation in the kidney tubule? Journal Of Clinical Investigation 2005, 115: 1705-1708. PMID: 16007248, PMCID: PMC1159151, DOI: 10.1172/jci25540.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Marrow CellsCell DifferentiationEpithelial CellsHumansKidney TubulesMiceModels, BiologicalRegenerationReperfusion InjuryConceptsBone marrow-derived cellsMarrow-derived cellsTubule repairBone marrowEpithelial cellsStem cellsIschemic injuryRenal interstitiumTubular cellsRenal epithelial cellsCell lossTubular segmentsRenal cellsKidney tubulesMajority of dataEndogenous cellsHigh rateRecent reportsKidneyMarrowRepairCellsTubulesProliferationStem cell nicheEngraftment of Bone Marrow‐Derived Epithelial Cells
Krause DS. Engraftment of Bone Marrow‐Derived Epithelial Cells. Annals Of The New York Academy Of Sciences 2005, 1044: 117-124. PMID: 15958704, DOI: 10.1196/annals.1349.015.Peer-Reviewed Original ResearchConceptsBone marrow-derived cellsMarrow-derived cellsEpithelial cellsMarrow-derived epithelial cellsNonhematopoietic cellsLevel of engraftmentCell plasticityStem cell plasticityAllogeneic settingAdult stem cell plasticityBM cellsBuccal mucosaGastrointestinal tractBone marrowTissue damageEngraftmentFunctional epithelial cellsTherapeutic relevanceNonhematopoietic cell typesPrecursor cellsMost reportsDiseased organsLungMarrowDifferent phenotypesEngraftment of bone marrow-derived epithelial cells
Van Arnam JS, Herzog E, Grove J, Bruscia E, Ziegler E, Swenson S, Krause DS. Engraftment of bone marrow-derived epithelial cells. Stem Cell Reviews And Reports 2005, 1: 21-27. PMID: 17132871, DOI: 10.1385/scr:1:1:021.Peer-Reviewed Original ResearchConceptsBM-derived cellsEpithelial cellsBM transplantationBone marrow-derived epithelial cellsMarrow-derived epithelial cellsPreclinical mouse modelsType II pneumocytesHematopoietic systemGene therapyFalse-positive cellsGastrointestinal tractHost epithelial cellsMouse modelFemale recipientsPositive cellsBone marrowCre-lox systemTherapeutic potentialTherapeutic useTissue-specific markersTransplantationTherapyPotential gene therapy applicationsCellsHuman diseases
2004
Bone Marrow-Derived Cells Contribute to Epithelial Engraftment during Wound Healing
Borue X, Lee S, Grove J, Herzog EL, Harris R, Diflo T, Glusac E, Hyman K, Theise ND, Krause DS. Bone Marrow-Derived Cells Contribute to Epithelial Engraftment during Wound Healing. American Journal Of Pathology 2004, 165: 1767-1772. PMID: 15509544, PMCID: PMC1618655, DOI: 10.1016/s0002-9440(10)63431-1.Peer-Reviewed Original ResearchConceptsBone marrow-derived cellsEpithelial cellsMarrow-derived epithelial cellsEngraftment of BMDCsDonor-derived cellsMarrow-derived cellsWound healingDegree of engraftmentLevel of engraftmentAbsence of injuryEarly wound healingFemale miceBone marrowCytokeratin 5Cre-lox systemEngraftmentSkin damageWound edgeKeratinocytesInjuryWound siteTransit-amplifying cellsStem cellsEpidermal stem cellsRecent findingsLack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia
Harris RG, Herzog EL, Bruscia EM, Grove JE, Van Arnam JS, Krause DS. Lack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia. Science 2004, 305: 90-93. PMID: 15232107, DOI: 10.1126/science.1098925.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GalactosidaseBone Marrow CellsBone Marrow TransplantationCell DifferentiationCell FusionCobra Cardiotoxin ProteinsElapid VenomsEpithelial CellsFemaleGreen Fluorescent ProteinsHepatocytesKeratinocytesKeratinsLuminescent ProteinsMaleMiceMice, TransgenicMuscle CellsRadiation, IonizingRecombinasesRecombination, GeneticReverse Transcriptase Polymerase Chain ReactionStem CellsX ChromosomeY ChromosomeConceptsCell-cell fusionBone marrow-derived cellsCre/lox systemGreen fluorescent protein expressionFluorescent protein expressionEpithelial cellsDevelopmental plasticityLox systemCell fusionProtein expressionMarrow-derived cellsTransgenic miceCellsBone marrowFusionFusion requirementsPlasticityExpression
2002
Radiation pneumonitis in mice A severe injury model for pneumocyte engraftment from bone marrow
Theise ND, Henegariu O, Grove J, Jagirdar J, Kao PN, Crawford JM, Badve S, Saxena R, Krause DS. Radiation pneumonitis in mice A severe injury model for pneumocyte engraftment from bone marrow. Experimental Hematology 2002, 30: 1333-1338. PMID: 12423687, DOI: 10.1016/s0301-472x(02)00931-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersBone Marrow TransplantationCell DifferentiationCell LineageEpithelial CellsFemaleGraft SurvivalIn Situ Hybridization, FluorescenceLungMaleMiceModels, AnimalPulmonary AlveoliPulmonary Surfactant-Associated Protein BRadiation ChimeraRadiation PneumonitisRNA, MessengerStem Cell TransplantationStem CellsY ChromosomeConceptsBone marrow transplantType II pneumocytesBone marrow cellsFemale miceLethal irradiationAge-matched male donorsWhole bone marrow transplantsMarrow cellsDay 5 posttransplantAlveolar lining cellsFluorescence-activated cell sorterSevere injury modelType I cellsAlveolar breakdownEntire alveoliRadiation pneumonitisB messenger RNAHistologic evidenceMarrow transplantAcute injuryMonth 2Injury modelLung tissueLining cellsBone marrow
2001
Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Krause D, Theise N, Collector M, Henegariu O, Hwang S, Gardner R, Neutzel S, Sharkis S. Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell. Cell 2001, 105: 369-377. PMID: 11348593, DOI: 10.1016/s0092-8674(01)00328-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CD34Antigens, LyBone Marrow CellsCell LineageCell MovementEpithelial CellsFemaleFluorescent DyesHematopoietic Stem Cell TransplantationHematopoietic Stem CellsHumansImmunohistochemistryIn Situ Hybridization, FluorescenceIntestine, SmallKeratinsLungMaleMembrane ProteinsMiceMice, KnockoutOrganic ChemicalsPulmonary SurfactantsStem CellsY ChromosomeConceptsLong-term repopulationSingle bone marrowMulti-lineage engraftmentAdult bone marrow cellsProperties of HSCHematopoietic stemSecondary hostsGenetic diseasesStem cellsBone marrow cellsExpression increasesDifferentiative capacityBone marrowEpithelial cellsSerial transplantationRare cellsTissue repairMarrow cellsCellsDifferentiationHostSecondary recipientsGI tractPhenotypeMarrow