2023
Electrical properties of dendritic spines
Zecevic D. Electrical properties of dendritic spines. Biophysical Journal 2023, 122: 4303-4315. PMID: 37837192, PMCID: PMC10698282, DOI: 10.1016/j.bpj.2023.10.008.Peer-Reviewed Original ResearchConceptsDendritic spinesIntracellular calcium concentration changesCortical pyramidal neuronsExcitatory synaptic transmissionCalcium concentration changesBasal dendritesPyramidal neuronsMushroom spinesSpine synapsesSynaptic transmissionBrain slicesVoltage-sensitive dyeNervous systemSpine neckSignificant physiological roleSynaptic signalingTwo-photon uncagingElectrical compartmentsSpineOnly experimental studiesBiochemical compartmentalizationPhysiological roleDifferent preparationsAnatomical structuresAdequate sensitivity
2017
The stochastic nature of action potential backpropagation in apical tuft dendrites
Short SM, Oikonomou KD, Zhou WL, Acker CD, Popovic MA, Zecevic D, Antic SD. The stochastic nature of action potential backpropagation in apical tuft dendrites. Journal Of Neurophysiology 2017, 118: 1394-1414. PMID: 28566465, PMCID: PMC5558024, DOI: 10.1152/jn.00800.2016.Peer-Reviewed Original ResearchConceptsDendritic CaPyramidal neuronsApical tuftCortical pyramidal neuronsAction potential backpropagationRat brain slicesSpontaneous synaptic inputsDifferent cortical layersVoltage-gated CaAP backpropagationAP burstsGlutamate iontophoresisSynaptic contactsSomatic APsApical trunkAP frequencyBrain slicesDendritic physiologySynaptic inputsCortical layersChannel inactivationLocal NaNeuronsSpike-timing dependent plasticityTrials
2015
Combining Membrane Potential Imaging with Other Optical Techniques
Jaafari N, Vogt KE, Saggau P, Leslie LM, Zecevic D, Canepari M. Combining Membrane Potential Imaging with Other Optical Techniques. Advances In Experimental Medicine And Biology 2015, 859: 103-125. PMID: 26238050, PMCID: PMC5675139, DOI: 10.1007/978-3-319-17641-3_4.Peer-Reviewed Original ResearchConceptsMembrane potential imagingOptical techniquesPotential imagingUncaging techniquesChannelrhodopsin stimulationVoltage-sensitive dyeVariety of applicationsSpatial mappingElectrical signalsImportant applicationsVoltageFluorescence intensityImagingParticular moleculeSignalsMembrane potential changesGateNovel investigationOptogeneticsMeasurementsIntensityTechniqueCurrentImaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines
Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg BM, Grinvald A, Antic SD, Canepari M, Zecevic D. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines. Advances In Experimental Medicine And Biology 2015, 859: 57-101. PMID: 26238049, PMCID: PMC5671121, DOI: 10.1007/978-3-319-17641-3_3.Peer-Reviewed Original ResearchConceptsIndividual neuronsMembrane potential changesVoltage-sensitive dye recordingAction potential initiationIndividual dendritic spinesSite of originAxon collateralsIndividual nerve cellsMembrane potential transientsVoltage-sensitive dyeDendritic spinesRegional electrical propertiesDendritic treeNerve cellsNeuronal processesSingle axonsPotential initiationComplex operational unitsBehavioral modificationNeuronal network analysisNeuronsInput-output functionMultisite recordings
2012
Cortical Dendritic Spine Heads Are Not Electrically Isolated by the Spine Neck from Membrane Potential Signals in Parent Dendrites
Popovic MA, Gao X, Carnevale NT, Zecevic D. Cortical Dendritic Spine Heads Are Not Electrically Isolated by the Spine Neck from Membrane Potential Signals in Parent Dendrites. Cerebral Cortex 2012, 24: 385-395. PMID: 23054810, PMCID: PMC3888368, DOI: 10.1093/cercor/bhs320.Peer-Reviewed Original Research
2010
Rapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons
Holthoff K, Zecevic D, Konnerth A. Rapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons. The Journal Of Physiology 2010, 588: 1085-1096. PMID: 20156851, PMCID: PMC2852997, DOI: 10.1113/jphysiol.2009.184960.Peer-Reviewed Original ResearchConceptsRapid time courseAction potentialsDendritic spinesIndividual dendritic spinesLayer 5 pyramidal neuronsTime courseTiming-dependent synaptic plasticityDendritic branchesCentral mammalian neuronsTerminal dendritic branchesVisual cortex neuronsTerminal branchesSomatic action potentialsIndividual spinesRemote dendritesPyramidal neuronsApical dendritesCortex neuronsExcitatory synapsesSupralinear integrationSynaptic inputsVoltage-sensitive dyeJuvenile miceSynaptic plasticityVisual cortex
2003
Imaging Nervous System Activity with Voltage‐Sensitive Dyes
Zecevic D, Djurisic M, Cohen LB, Antic S, Wachowiak M, Falk CX, Zochowski MR. Imaging Nervous System Activity with Voltage‐Sensitive Dyes. Current Protocols In Neuroscience 2003, 23: 6.17.1-6.17.29. PMID: 18428582, DOI: 10.1002/0471142301.ns0617s23.Peer-Reviewed Original ResearchConceptsNumber of photonsVoltage-sensitive dyeVoltage-sensitive dye measurementsLight sourceShot noiseOptical recordingOptical recording methodSynaptic inputsChoice of dyeSimultaneous measurementNervous system activityAction potential outputAplysia abdominal ganglionLarge signalNoise ratioAbdominal ganglionNervous systemPhotonsDye measurementsOpticsMotor outputSystem activitySensory stimuliMeasurementsVertebrate brain