2015
Combining Membrane Potential Imaging with Other Optical Techniques
Jaafari N, Vogt KE, Saggau P, Leslie LM, Zecevic D, Canepari M. Combining Membrane Potential Imaging with Other Optical Techniques. Advances In Experimental Medicine And Biology 2015, 859: 103-125. PMID: 26238050, PMCID: PMC5675139, DOI: 10.1007/978-3-319-17641-3_4.Peer-Reviewed Original ResearchConceptsMembrane potential imagingOptical techniquesPotential imagingUncaging techniquesChannelrhodopsin stimulationVoltage-sensitive dyeVariety of applicationsSpatial mappingElectrical signalsImportant applicationsVoltageFluorescence intensityImagingParticular moleculeSignalsMembrane potential changesGateNovel investigationOptogeneticsMeasurementsIntensityTechniqueCurrent
2013
Combining calcium imaging with other optical techniques.
Canepari M, Zecevic D, Vogt KE, Ogden D, De Waard M. Combining calcium imaging with other optical techniques. Cold Spring Harbor Protocols 2013, 2013: 1125-31. PMID: 24298025, PMCID: PMC5225261, DOI: 10.1101/pdb.top066167.Peer-Reviewed Original Research
2012
Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices.
Popovic M, Gao X, Zecevic D. Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices. Journal Of Visualized Experiments 2012, e4261. PMID: 23222505, PMCID: PMC3565854, DOI: 10.3791/4261.Peer-Reviewed Original ResearchConceptsLaser light sourceSub-micrometer resolutionLight sourceOptical recordingDetectable pharmacological effectsCurrent sensitivityVoltage-sensitive dyeSubthreshold eventsElectrical propertiesDetailed measurementsMultiple-site optical recordingVoltage-sensitive dye recordingFluorescence signalOptimal wavelengthsMembrane potential transientsElectrical signalsIndividual dendritic spinesExperimental evidenceWavelengthMeasurement oneMeasurementsBroadeningDye loadingPotential transientsBrightness
2010
Combined Voltage and Calcium Imaging and Signal Calibration
Canepari M, Saggau P, Zecevic D. Combined Voltage and Calcium Imaging and Signal Calibration. 2010, 43-52. DOI: 10.1007/978-1-4419-6558-5_4.Peer-Reviewed Original ResearchOptical measurementsVoltage-sensitive dye signalsVoltage imagingAbsolute scaleCapability of voltageVoltage-sensitive dyeFluorescent voltage-sensitive dyeSignal calibrationDye signalsSpatial mappingVoltageMeasurementsVoltage signalImagingMembrane potential changesSignalsCalibrationCombined voltageFundamental problem
2009
Imaging Membrane Potential with Voltage-Sensitive Dyes
Vucinic D, Kosmidis E, Falk C, Cohen L, Loew L, Djurisic M, Zecevic D. Imaging Membrane Potential with Voltage-Sensitive Dyes. 2009, 132-160. DOI: 10.1093/oso/9780195150445.003.0006.Peer-Reviewed Original ResearchMembrane-bound dyeOptical signalSecond harmonic generationVoltage-sensitive dyeDifferent optical propertiesHarmonic generationOptical measurementsOptical propertiesRaman absorptionOptical recordingRise timeSimultaneous measurementAbsorptionFigure 6.1BirefringenceFluorescenceMeasurementsDichroismDyeMolecular probesSignalsSquidKinds of results
2003
Imaging Nervous System Activity with Voltage‐Sensitive Dyes
Zecevic D, Djurisic M, Cohen LB, Antic S, Wachowiak M, Falk CX, Zochowski MR. Imaging Nervous System Activity with Voltage‐Sensitive Dyes. Current Protocols In Neuroscience 2003, 23: 6.17.1-6.17.29. PMID: 18428582, DOI: 10.1002/0471142301.ns0617s23.Peer-Reviewed Original ResearchConceptsNumber of photonsVoltage-sensitive dyeVoltage-sensitive dye measurementsLight sourceShot noiseOptical recordingOptical recording methodSynaptic inputsChoice of dyeSimultaneous measurementNervous system activityAction potential outputAplysia abdominal ganglionLarge signalNoise ratioAbdominal ganglionNervous systemPhotonsDye measurementsOpticsMotor outputSystem activitySensory stimuliMeasurementsVertebrate brain[20] Optical monitoring of neural activity using voltage-sensitive dyes
Djurisic M, Zochowski M, Wachowiak M, Falk CX, Cohen LB, Zecevic D. [20] Optical monitoring of neural activity using voltage-sensitive dyes. Methods In Enzymology 2003, 361: 423-451. PMID: 12624923, DOI: 10.1016/s0076-6879(03)61022-0.Peer-Reviewed Original ResearchConceptsOptical monitoringSecond harmonic generationVoltage-sensitive dyeDifferent optical propertiesMembrane-bound dyeHarmonic generationOptical propertiesOptical measurementsFluorescence resonance energy transferRaman absorptionEnergy transferResonance energy transferSimultaneous measurementAbsorptionBirefringenceMeasurementsDichroismDyeMolecular probesPopulation signalsFluorescenceProbe