Prostate-specific membrane antigen: a novel folate hydrolase in human prostatic carcinoma cells.
Pinto J, Suffoletto B, Berzin T, Qiao C, Lin S, Tong W, May F, Mukherjee B, Heston W. Prostate-specific membrane antigen: a novel folate hydrolase in human prostatic carcinoma cells. Clinical Cancer Research 1996, 2: 1445-51. PMID: 9816319.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, SurfaceCarboxypeptidasesCell FractionationCell MembraneFolic AcidGamma-Glutamyl HydrolaseGlutamate Carboxypeptidase IIHumansHydrogen-Ion ConcentrationHydrolasesImmunohistochemistryMaleMethotrexatePolyglutamic AcidProstatic NeoplasmsPteroylpolyglutamic AcidsSubstrate SpecificityTumor Cells, CulturedConceptsFolate hydrolase activityProtein prostate-specific membrane antigenHuman prostate cancerPC-3 cellsProstate cancerMembrane-bound enzymeHydrolase activityMonoclonal antibodiesLNCaP cellsTransfection of PC-3 cellsResistance to methotrexate therapyClass II membrane proteinHuman prostate cancer cell linesTreatment of prostate cancerMembrane fractionProstate cancer cell linesHuman prostate carcinoma cellsLNCaP cell membranesProstate-specific membraneHuman prostate adenocarcinoma cellsPhase 3 trialProstate carcinoma cellsProstate adenocarcinoma cellsAbsence of thiol reagentsEukaryotic expression vector