2009
Cholera toxin inhibits IL-12 production and CD8α+ dendritic cell differentiation by cAMP-mediated inhibition of IRF8 function
la Sala A, He J, Laricchia-Robbio L, Gorini S, Iwasaki A, Braun M, Yap GS, Sher A, Ozato K, Kelsall B. Cholera toxin inhibits IL-12 production and CD8α+ dendritic cell differentiation by cAMP-mediated inhibition of IRF8 function. Journal Of Experimental Medicine 2009, 206: 1227-1235. PMID: 19487420, PMCID: PMC2715075, DOI: 10.1084/jem.20080912.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8 AntigensCD8-Positive T-LymphocytesCell DifferentiationCells, CulturedCholera ToxinCyclic AMPDendritic CellsFemaleGTP-Binding Protein alpha Subunits, GsHumansInterferon Regulatory Factor-1Interferon Regulatory FactorsInterferon-gammaInterleukin-12Interleukin-12 Subunit p40MiceMice, Inbred BALB CSpleenToxoplasmosisConceptsIL-12 productionDendritic cellsPlasmacytoid DCsCholera toxinSerum IL-12 levelsIL-12 levelsPlasmacytoid dendritic cellsConventional dendritic cellsIL-12p40 promoterDendritic cell differentiationConventional DCsP40 gene expressionBone marrow cellsInterferon regulatory factor 8Regulatory factor 8Th1 responseDC differentiationIL-12p35Lymphoid organsToxoplasma gondiiMarrow cellsDibutyryl cAMPIRF8Factor 8Common mechanism
2004
Recognition of single-stranded RNA viruses by Toll-like receptor 7
Lund JM, Alexopoulou L, Sato A, Karow M, Adams NC, Gale NW, Iwasaki A, Flavell RA. Recognition of single-stranded RNA viruses by Toll-like receptor 7. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 5598-5603. PMID: 15034168, PMCID: PMC397437, DOI: 10.1073/pnas.0400937101.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAntigens, DifferentiationBone Marrow CellsChick EmbryoChloroquineCytokinesDendritic CellsEndosomesInterferon-alphaMacrophagesMembrane GlycoproteinsMiceMice, KnockoutMyeloid Differentiation Factor 88OrthomyxoviridaePeritoneumReceptors, Cell SurfaceReceptors, ImmunologicRhabdoviridae InfectionsRNA, ViralSpleenToll-Like Receptor 7Vesicular stomatitis Indiana virusConceptsVesicular stomatitis virusRNA virusesHigh CpG contentGenomes of virusesToll-like receptorsStomatitis virusMammalian genomesGenomic nucleic acidsAdaptor protein MyD88Endocytic pathwayLigand recognitionCpG contentViral infectionTLR adaptor protein MyD88Innate immune responseToll-like receptor 7Molecular signaturesPlasmacytoid dendritic cellsInnate immune cellsProduction of cytokinesGenomeProtein MyD88Types of pathogensNucleic acidsVivo infection
2003
CD11b+ Peyer’s Patch Dendritic Cells Secrete IL-6 and Induce IgA Secretion from Naive B Cells
Sato A, Hashiguchi M, Toda E, Iwasaki A, Hachimura S, Kaminogawa S. CD11b+ Peyer’s Patch Dendritic Cells Secrete IL-6 and Induce IgA Secretion from Naive B Cells. The Journal Of Immunology 2003, 171: 3684-3690. PMID: 14500666, DOI: 10.4049/jimmunol.171.7.3684.Peer-Reviewed Original ResearchConceptsPP dendritic cellsNaive B cellsDendritic cellsIL-6B cellsIgA secretionDC subsetsIgA productionPeyer's patch dendritic cellsSecrete IL-6Exogenous IL-6Cell coculture systemDC populationsLymphoid organsCytokine secretionAb productionT cellsHigh levelsSpleenSecretionCoculture systemCellsIgAUnique roleDistinct capacities
2001
Unique Functions of CD11b+, CD8α+, and Double-Negative Peyer’s Patch Dendritic Cells
Iwasaki A, Kelsall B. Unique Functions of CD11b+, CD8α+, and Double-Negative Peyer’s Patch Dendritic Cells. The Journal Of Immunology 2001, 166: 4884-4890. PMID: 11290765, DOI: 10.4049/jimmunol.166.8.4884.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDB7-1 AntigenB7-2 AntigenCD8 AntigensCell LineageCell SeparationDendritic CellsEpithelial CellsEpitopes, T-LymphocyteFemaleHistocompatibility Antigens Class IIImmunophenotypingInterferon-gammaInterleukin-10Interleukin-12Interleukin-4Lectins, C-TypeLymphocyte ActivationLymphocyte SubsetsMacrophage-1 AntigenMembrane GlycoproteinsMiceMice, Inbred BALB CMice, Inbred C57BLMice, TransgenicMinor Histocompatibility AntigensMyeloid CellsPeyer's PatchesReceptors, Cell SurfaceSpleenT-LymphocytesUp-RegulationConceptsMyeloid dendritic cellsDendritic cellsCD40 ligand trimerDC subsetsIL-12p70IL-10T cellsPeyer's patch dendritic cellsIFN-gamma productionSoluble CD40 ligand trimerMucosal lymphoid tissuesNaive T cellsFollicle-associated epitheliumMurine Peyer's patchesNonmucosal sitesDC subpopulationsSubepithelial domeIL-4Lymphoid tissuePeyer's patchesMicrobial stimuliInterfollicular regionsIFN-gammaSurface phenotypeMucosal tissues
2000
Primary Role for GI Protein Signaling in the Regulation of Interleukin 12 Production and the Induction of T Helper Cell Type 1 Responses
He J, Gurunathan S, Iwasaki A, Ash-Shaheed B, Kelsall B. Primary Role for GI Protein Signaling in the Regulation of Interleukin 12 Production and the Induction of T Helper Cell Type 1 Responses. Journal Of Experimental Medicine 2000, 191: 1605-1610. PMID: 10790434, PMCID: PMC2213427, DOI: 10.1084/jem.191.9.1605.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine Diphosphate RiboseAnimalsCD8 AntigensCell DifferentiationDendritic CellsGTP-Binding Protein alpha Subunits, Gi-GoInterferon-gammaInterleukin-10Interleukin-12Interleukin-4Leishmaniasis, CutaneousLymph NodesMiceMice, Inbred BALB CMice, Mutant StrainsPertussis ToxinProtein Processing, Post-TranslationalSignal TransductionSpleenTh1 CellsTumor Necrosis Factor-alphaVirulence Factors, BordetellaConceptsPertussis toxinGi-protein signalingTh1 responseIL-12T helper cell type 1 responseGi proteinsNormal BALB/c miceBALB/c miceLymphoid dendritic cellsIL-12 productionInterleukin-12 productionType 1 responseCapacity of splenocytesIL-12 p40Tumor necrosis factorRegulation of interleukinT cell differentiationNonmicrobial stimuliDendritic cellsIL-10Lymph nodesC miceTNF-alphaNecrosis factorProtein signalingLocalization of Distinct Peyer's Patch Dendritic Cell Subsets and Their Recruitment by Chemokines Macrophage Inflammatory Protein (Mip)-3α, Mip-3β, and Secondary Lymphoid Organ Chemokine
Iwasaki A, Kelsall B. Localization of Distinct Peyer's Patch Dendritic Cell Subsets and Their Recruitment by Chemokines Macrophage Inflammatory Protein (Mip)-3α, Mip-3β, and Secondary Lymphoid Organ Chemokine. Journal Of Experimental Medicine 2000, 191: 1381-1394. PMID: 10770804, PMCID: PMC2193144, DOI: 10.1084/jem.191.8.1381.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceChemokine CCL19Chemokine CCL20Chemokines, CCChemotaxisDendritic CellsDNA PrimersFemaleGene ExpressionIn Situ HybridizationMacrophage Inflammatory ProteinsMiceMice, Inbred BALB CMicroscopy, ConfocalModels, BiologicalPeyer's PatchesReceptors, CCR6Receptors, CCR7Receptors, ChemokineRNA, MessengerSpleenConceptsDendritic cell subsetsInterfollicular regionsDC subsetsCell subsetsInflammatory proteinPeyer's patchesSecondary lymphoid organ chemokineChemokine macrophage inflammatory proteinSplenic DC subsetsSubepithelial dome regionDistinct dendritic cell subsetsRole of chemokinesT cell responsesMacrophage inflammatory proteinT-cell regionsFollicle-associated epitheliumMurine Peyer's patchesTranscriptase-polymerase chain reaction analysisFunctional CCR7Lymphoid DCsMIP-3βPP DCsMyeloid DCsCCR7 expressionPolymerase chain reaction analysis
1999
Freshly Isolated Peyer's Patch, but Not Spleen, Dendritic Cells Produce Interleukin 10 and Induce the Differentiation of T Helper Type 2 Cells
Iwasaki A, Kelsall B. Freshly Isolated Peyer's Patch, but Not Spleen, Dendritic Cells Produce Interleukin 10 and Induce the Differentiation of T Helper Type 2 Cells. Journal Of Experimental Medicine 1999, 190: 229-240. PMID: 10432286, PMCID: PMC2195574, DOI: 10.1084/jem.190.2.229.Peer-Reviewed Original ResearchConceptsPP dendritic cellsDendritic cellsNaive T cellsPeyer's patchesT cellsIL-10DC populationsImmune responseT cell receptor transgenic miceAllogeneic T cell proliferationT helper type 2 cellsT helper cell responsesMajor histocompatibility complex (MHC) class II moleculesIsolated Peyer's patchesSurface phenotypic analysisVivo dendritic cellsHelper cell responsesIL-10 secretionPrime T cellsDistinct immune responsesReceptor transgenic miceAntigen-presenting cellsIFN-gamma productionProduction of interleukinT cell proliferation