2020
Molecular Characterization and Experimental Utility of Monoclonal Antibodies with Specificity for Aliphatic Di- and Polyisocyanates
Wisnewski AV, Liu J. Molecular Characterization and Experimental Utility of Monoclonal Antibodies with Specificity for Aliphatic Di- and Polyisocyanates. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 2020, 39: 66-73. PMID: 32302507, PMCID: PMC7310211, DOI: 10.1089/mab.2020.0006.Peer-Reviewed Original ResearchConceptsBALB/c miceMonoclonal antibodiesOccupational asthmaC miceSerologic detectionDifferent carrier proteinsNew mAbsEpitope specificityUseful research reagentsDisease preventionImmune recognitionStable hybridomasWestern blotAlbumin adductsMouse skinMAbsSurveillance approachSurveillance effortsAntibodiesMolecular determinantsHybridomasAliphatic diComplementary determining regionsMolecular characterizationResearch reagents
2016
The influence of diisocyanate antigen preparation methodology on monoclonal and serum antibody recognition
Hagerman LM, Law BF, Bledsoe TA, Hettick JM, Kashon ML, Lemons AR, Wisnewski AV, Siegel PD. The influence of diisocyanate antigen preparation methodology on monoclonal and serum antibody recognition. Journal Of Occupational And Environmental Hygiene 2016, 13: 829-839. PMID: 27124286, PMCID: PMC5016257, DOI: 10.1080/15459624.2016.1183013.Peer-Reviewed Original ResearchConceptsOccupational asthmaDifferent conjugatesPolyclonal antibody responsePanel of antigensSpecific antibody clonesHuman serumMDI conjugationPolyclonal serum antibodiesSpecific IgEAntibody responseSerum antibodiesSerologic detectionStandard antigenMonoclonal antibodiesAntibody clonesAntibodiesAsthmaSignificant differencesAntigenSerumMAbsAntibody recognitionAntigenicityMinimal differencesConjugates
2013
Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate
Wisnewski AV, Liu J. Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate. Molecular Immunology 2013, 54: 233-237. PMID: 23295252, PMCID: PMC3563841, DOI: 10.1016/j.molimm.2012.11.017.Peer-Reviewed Original ResearchConceptsEpitope specificityAutologous serum proteinsLow molecular weight chemicalsOccupational asthmaOccupational allergensMolecular determinantsDisease pathogenesisSecreting clonesCDR3 regionWestern blotGene usageMonoclonal antibodiesMajor causeWeight chemicalsIgG1 mAbGermline gene usageHuman albuminImmune specificityMAbsSerum proteinsELISADiagnostic reagentsUseful diagnostic reagentHybridomasSomatic hypermutation
1998
Functional and Molecular Characterization of Human Monoclonal Antibody Reactive with the Immunodominant Region of HIV Type 1 Glycoprotein 41
Cavacini L, Emes C, Wisnewski A, Power J, Lewis G, Montefiori D, Posner M. Functional and Molecular Characterization of Human Monoclonal Antibody Reactive with the Immunodominant Region of HIV Type 1 Glycoprotein 41. AIDS Research And Human Retroviruses 1998, 14: 1271-1280. PMID: 9764911, DOI: 10.1089/aid.1998.14.1271.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, MonoclonalBlotting, WesternCloning, MolecularComplement System ProteinsGenes, ImmunoglobulinHIV Envelope Protein gp120HIV Envelope Protein gp41HIV InfectionsHIV-1HumansImmunodominant EpitopesImmunoglobulin Heavy ChainsImmunoglobulin Light ChainsMiceMolecular Sequence DataSequence Analysis, DNAConceptsHuman monoclonal antibodyHuman Monoclonal Antibody ReactiveHIV-1-infected individualsImmunodominant regionCD4-binding siteAntigen-driven responseComplement-dependent mannerHIV Type 1 Glycoprotein 41Presence of complementMonoclonal Antibodies ReactiveLaboratory isolatesSoluble CD4Nonprotective antibodiesPrecursor gp160Antibody reactiveAntigen specificityViral infectionGp41 regionExtensive somatic mutationCytometric analysisAntibody clonotypesMonoclonal antibodiesPepscan analysisUninfected cellsFunctional activity
1997
In vitro inhibition of Cryptosporidium parvum infection by human monoclonal antibodies
Elliot BC, Wisnewski AV, Johnson J, Fenwick-Smith D, Wiest P, Hamer D, Kresina T, Flanigan TP. In vitro inhibition of Cryptosporidium parvum infection by human monoclonal antibodies. Infection And Immunity 1997, 65: 3933-3935. PMID: 9284173, PMCID: PMC175560, DOI: 10.1128/iai.65.9.3933-3935.1997.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyC. parvum infectionParvum infectionEnzyme-linked immunosorbent assayMonoclonal antibodiesHuman immunodeficiency virus-seronegative womenCell linesHuman enterocyte cell lineHigher specific antibody titersCryptosporidium parvum infectionAnti-Cryptosporidium antibodiesPeripheral blood lymphocytesSpecific antibody titersMonoclonal cell linesEnterocyte cell lineAntibody-secreting clonesC. parvum sporozoitesImmunoglobulin therapyAntibody titersBlood lymphocytesFatal illnessPotential therapyHypo-osmolar electrofusionTumor cell linesInfection
1994
Human monoclonal antibodies heterogeneously express a human cross-reactive idiotype associated with immune function in Schistosoma japonicum infection.
Wisnewski AV, Olds GR, Kresina TF. Human monoclonal antibodies heterogeneously express a human cross-reactive idiotype associated with immune function in Schistosoma japonicum infection. Human Antibodies 1994, 5: 178-82. PMID: 7756581.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyEpstein-Barr virusSchistosoma japonicum infectionCross-reactive idiotypeJaponicum infectionImmune functionMonoclonal antibodiesSoluble egg antigenS. japonicum infectionWestern blot profilesSchistosomiasis patientsBarr virusEgg antigenHuman idiotypeIdiotype expressionReactive idiotypeMultiple antigensSerological analysisS. japonicumIdiotypeInfectionAntigenHeterogeneous expressionLevel of suppressionPatients
1992
Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens
Klock G, Wisnewski AV, el-Bassiouni EA, Ramadan MI, Gessner P, Zimmermann U, Kresina TF. Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 1992, 11: 469-481. PMID: 1398684, DOI: 10.1089/hyb.1992.11.469.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyMonoclonal antibodiesIgG antibodiesParasite antigensHypo-osmolar electrofusionS. mansoniSchistosoma mansoni wormsCell linesWestern blot analysisEgg antigenParasitic antigensAntigen reactivityAntigenAntibodiesSplenocytesBlot analysisHybridomasCandidate moleculesMansoniHybridoma generationMitogenCell fusionSomatic cell fusionGreater numberELISA
1991
Screening of murine monoclonal antibodies against living schistosomula of Schistosoma mansoni by radioimmunoassay
Wiest P, Wisnewski A, Johnson J, Ramirez B, Kresina T, Olds G. Screening of murine monoclonal antibodies against living schistosomula of Schistosoma mansoni by radioimmunoassay. International Journal For Parasitology 1991, 21: 449-454. PMID: 1917285, DOI: 10.1016/0020-7519(91)90102-d.Peer-Reviewed Original ResearchConceptsMurine monoclonal antibodiesSurface antigenSoluble adult worm antigenSchistosoma mansoniMonoclonal antibodiesS. mansoni paramyosinS. mansoni infectionSoluble egg antigenAdult worm antigenEnzyme-linked immunosorbentMansoni infectionEgg antigenLung stageWorm antigenS. mansoniSecrete antibodiesAntigenSchistosomulaAdult wormsAntibodiesRadioimmunoassayMansoniImmunoblot analysisOld parasitesMurine hybridomasAn immunoregulatory human monoclonal antibody in schistosomiasis japonica
Kresina T, Guan X, Posner M, Wisnewski A, Olds G. An immunoregulatory human monoclonal antibody in schistosomiasis japonica. Human Antibodies 1991, 2: 42-45. PMID: 1651785, DOI: 10.3233/hab-1991-2107.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyMonoclonal antibodiesEgg antigenAntigen-induced blastogenesisAdult wormsEpstein-Barr virusSoluble egg antigenOS cell linesWestern blot analysisChronic schistosomiasisSoluble eggBarr virusChinese patientsChronic diseasesAntibody-producing hybridomasAntigen reactivitySchistosomiasis japonicaCarbohydrate antigensWestern blotDecompression operationMurine splenocytesSplenocytesAntibodiesAntigenSchistosoma japonicum