Salil Garg, MD, PhD
Assistant Professor of Laboratory MedicineCards
About
Research
Publications
2024
The dynamics of hematopoiesis over the human lifespan
Li H, Côté P, Kuoch M, Ezike J, Frenis K, Afanassiev A, Greenstreet L, Tanaka-Yano M, Tarantino G, Zhang S, Whangbo J, Butty V, Moiso E, Falchetti M, Lu K, Connelly G, Morris V, Wang D, Chen A, Bianchi G, Daley G, Garg S, Liu D, Chou S, Regev A, Lummertz da Rocha E, Schiebinger G, Rowe R. The dynamics of hematopoiesis over the human lifespan. Nature Methods 2024, 22: 422-434. PMID: 39639169, PMCID: PMC11908799, DOI: 10.1038/s41592-024-02495-0.Peer-Reviewed Original ResearchConceptsHematopoietic stem cellsHematopoietic stemProgenitor cellsClassification of acute myeloid leukemiaDifferentiation of hematopoietic stem cellsAssociated with poor prognosisAcute myeloid leukemiaHuman hematopoietic stemWave of hematopoiesisGene expression networksMyeloid leukemiaPoor prognosisLineage outputMultilineage capacityDynamics of hematopoiesisCell ontogenyStem cellsLineage primingFate decisionsModel organismsTranscriptomic statesExpression networksHuman lifespanTranscriptional programsHematopoiesis
2022
Transcription factor antagonism regulates heterogeneity in embryonic stem cell states
Hu S, Metcalf E, Mahat D, Chan L, Sohal N, Chakraborty M, Hamilton M, Singh A, Singh A, Lees J, Sharp P, Garg S. Transcription factor antagonism regulates heterogeneity in embryonic stem cell states. Molecular Cell 2022, 82: 4410-4427.e12. PMID: 36356583, PMCID: PMC9722640, DOI: 10.1016/j.molcel.2022.10.022.Peer-Reviewed Original ResearchConceptsGene expression heterogeneityMurine embryonic stem cellsCell statesGeneration of diverse cell typesExpression heterogeneityCell-type identityDiverse cell typesEmbryonic stem cell stateChromatin programmingStem cell stateTranscribed enhancersDevelopmental robustnessTranscription factor KLF4Expressed genesTranscription processDampened variationsGene expressionEmbryonic stem cellsGenesRegulatory programsPositive feedback networkCell typesZfp281CO bindingKinetics of switchingDevelopmental Deconvolution for Classification of Cancer Origin
Moiso E, Farahani A, Marble H, Hendricks A, Mildrum S, Levine S, Lennerz J, Garg S. Developmental Deconvolution for Classification of Cancer Origin. Cancer Discovery 2022, 12: 2566-2585. PMID: 36041084, PMCID: PMC9627133, DOI: 10.1158/2159-8290.cd-21-1443.Peer-Reviewed Original Research
2021
Acquired resistance to PRMT5 inhibition induces concomitant collateral sensitivity to paclitaxel
Mueller H, Fowler C, Dalin S, Moiso E, Udomlumleart T, Garg S, Hemann M, Lees J. Acquired resistance to PRMT5 inhibition induces concomitant collateral sensitivity to paclitaxel. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2024055118. PMID: 34408017, PMCID: PMC8403834, DOI: 10.1073/pnas.2024055118.Peer-Reviewed Original ResearchMeSH KeywordsAdenocarcinoma of LungAnimalsAntineoplastic AgentsCell Line, TumorCell ProliferationDrug Resistance, NeoplasmDrug SynergismEpigenesis, GeneticGene Expression Regulation, NeoplasticGene Knockdown TechniquesHumansLung NeoplasmsMiceMutationPaclitaxelProtein-Arginine N-MethyltransferasesStathminConceptsProtein arginine methyltransferase 5Stathmin-2Sensitivity to paclitaxelPRMT5 inhibitorsLung adenocarcinomaPRMT5 inhibitionResponse to taxane treatmentCell linesArginine methyltransferase 5Resistance to inhibitorsRegression of tumorsMicrotubule regulationLung adenocarcinoma lineAcquisition of resistanceCancer cell linesPotential clinical relevanceEpigenetic regulationHuman cancer cell linesBarcoding experimentsMechanisms of resistanceTaxane treatmentPreexisting populationTaxanes paclitaxelCollateral sensitivityLung adenocarcinoma cellsLineages of embryonic stem cells show non-Markovian state transitions
Udomlumleart T, Hu S, Garg S. Lineages of embryonic stem cells show non-Markovian state transitions. IScience 2021, 24: 102879. PMID: 34401663, PMCID: PMC8353490, DOI: 10.1016/j.isci.2021.102879.Peer-Reviewed Original ResearchEnhancers drive heterogeneity in embryonic stem cells
Hu S, Mahat DB, Chakraborty M, Hamilton M, Waterton S, Singh A, Lees J, Sharp P, Garg S. Enhancers drive heterogeneity in embryonic stem cells. Preprint. http://dx.doi.org/10.2139/ssrn.3836130.Peer-Reviewed Original Research
2020
MicroRNAs organize intrinsic variation into stem cell states
Chakraborty M, Hu S, Visness E, Del Giudice M, De Martino A, Bosia C, Sharp P, Garg S. MicroRNAs organize intrinsic variation into stem cell states. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 6942-6950. PMID: 32139605, PMCID: PMC7104302, DOI: 10.1073/pnas.1920695117.Peer-Reviewed Original ResearchConceptsGene expression statesGene regulatory networksCell statesRegulatory networksEmbryonic stem cellsExpression statesAbsence of external gradientsPromoter state transitionsDevelopmental gene expression programsDiverse array of cellsGene expression programsStem cell stateLoss of miRNAsMorphogen gradientsEnvironmental signalsPluripotent embryonic stem cellsTarget genesGenesExpression programsAdult vertebratesPluripotency genesDiverse arrayExternal gradientDistinct activitiesVariable miRNAs
2016
Single-cell variability guided by microRNAs
Garg S, Sharp P. Single-cell variability guided by microRNAs. Science 2016, 352: 1390-1391. PMID: 27313022, DOI: 10.1126/science.aag1097.Peer-Reviewed Original Research
2015
MHC Class II Presentation Is Controlled by the Lysosomal Small GTPase, Arl8b
Michelet X, Garg S, Wolf B, Tuli A, Ricciardi-Castagnoli P, Brenner M. MHC Class II Presentation Is Controlled by the Lysosomal Small GTPase, Arl8b. The Journal Of Immunology 2015, 194: 2079-2088. PMID: 25637027, DOI: 10.4049/jimmunol.1401072.Peer-Reviewed Original ResearchMeSH KeywordsADP-Ribosylation FactorsAnimalsAntigen PresentationAntigensBone Marrow CellsCD4-Positive T-LymphocytesCell LineChickensDendritic CellsEndosomesGene Expression RegulationHistocompatibility Antigens Class IILysosomesMiceMice, Inbred C57BLOvalbuminPrimary Cell CultureProtein TransportRNA, Small InterferingSignal TransductionSpleenConceptsDendritic cellsMHC-II presentationMHC II-peptide complexesII presentationII-peptide complexesT cellsCell surfaceCD4(+) T cellsPrime naive T cellsAg-presenting moleculesMHC class II presentationNaive T cellsMHC class IIT cell recognitionMHC II compartmentsGTPase Rab5Small GTPasesClass II presentationMHC II moleculesEndocytic vesiclesComplex formationArl8bAg deliveryRegulate formationExogenous AgChapter 7 RNAi screens of lysosomal trafficking
Garg S, Brenner M. Chapter 7 RNAi screens of lysosomal trafficking. Methods In Cell Biology 2015, 126: 119-138. PMID: 25665444, DOI: 10.1016/bs.mcb.2014.10.030.ChaptersConceptsShort hairpin RNA screenRNA interference screenStudy lysosome functionCargo traffickingInterference screenTrafficking proteinsGene candidatesMammalian cellsArray screeningRNA screeningLysosomal functionMicrobial killingAssay developmentLysosomesScreening systemPresentation to T cellsAntigen presentation to T cellsRNATraffickingProteinVesiclesScreeningT cellsBiologyCentral position
News & Links
News
- October 10, 2024Source: Yale News
Salil Garg, MD, Receives NIH 'High-Risk, High-Reward' Award
- October 09, 2024Source: Yale News
Six Yale Researchers Receive NIH ‘High-Risk, High-Reward’ Awards
- February 28, 2024
Grants Awarded at YCC • 2024
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