Lin Shao, PhD
Director, MicroscopyCards
About
Research
Publications
2025
Evolutionarily divergent Mycobacterium tuberculosis CTP synthase filaments are under selective pressure
Lynch E, Lu Y, Park J, Shao L, Kollman J, Rego E. Evolutionarily divergent Mycobacterium tuberculosis CTP synthase filaments are under selective pressure. Nature Communications 2025, 16: 5993. PMID: 40593557, PMCID: PMC12219555, DOI: 10.1038/s41467-025-60847-6.Peer-Reviewed Original ResearchConceptsCytidine triphosphate synthaseChromosome segregationCytidine triphosphateClinical isolatesClinical isolates of M. tuberculosisHigher-order filamentsCell wall synthesisTree of lifeIsolates of M. tuberculosisM. tuberculosisMutant enzymesWall synthesisCritical nucleotidesPyrimidine biosynthesisPositive selectionSelection pressureHigher-order structureActive conformationFilament formationProduct inhibitionUnusual architectureChromosomeMycobacterium tuberculosisEnzymeCellular techniquesThe bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage
Wang X, Xu P, Bentley-DeSousa A, Hancock-Cerutti W, Cai S, Johnson B, Tonelli F, Shao L, Talaia G, Alessi D, Ferguson S, De Camilli P. The bridge-like lipid transport protein VPS13C/PARK23 mediates ER–lysosome contacts following lysosome damage. Nature Cell Biology 2025, 27: 776-789. PMID: 40211074, PMCID: PMC12081312, DOI: 10.1038/s41556-025-01653-6.Peer-Reviewed Original ResearchConceptsDisease genesResponse to lysosomal damageSurface of lysosomesER–lysosome contactsParkinson's disease genesDelivery to lysosomesLipid transport proteinsLysosomal damageVPS13 proteinsLysosomal surfaceDisease proteinsGenetic studiesDamaged lysosomesVPS13CLysosomal stressLipid transportLysosomesInhibited stateMembrane perturbationRab7Lysosomal dysfunctionProteinVps13LipidGenesA quantitative ultrastructural timeline of nuclear autophagy reveals a role for dynamin-like protein 1 at the nuclear envelope
Mannino P, Perun A, Surovtsev I, Ader N, Shao L, Rodriguez E, Melia T, King M, Lusk C. A quantitative ultrastructural timeline of nuclear autophagy reveals a role for dynamin-like protein 1 at the nuclear envelope. Nature Cell Biology 2025, 27: 464-476. PMID: 39920277, PMCID: PMC11908896, DOI: 10.1038/s41556-025-01612-1.Peer-Reviewed Original ResearchConceptsInner nuclear membraneDynamin-like protein 1Membrane fissionNuclear envelopeMembrane fission stepNon-canonical rolesDouble-membrane vesiclesProtein 1Nuclear envelope remodelingLattice light-sheet microscopyFission stepDnm1Nuclear autophagyIntact nucleiPerinuclear spaceNuclear membraneAutophagic mechanismsNucleophagyCorrelative lightLight-sheet microscopyFissionElectron tomographyVesiclesVacuolesAtg11
2024
Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM
Ouyang Z, Wang Q, Li X, Dai Q, Tang M, Shao L, Gou W, Yu Z, Chen Y, Zheng B, Chen L, Ping C, Bi X, Xiao B, Yu X, Liu C, Chen L, Fan J, Huang X, Zhang Y. Elucidating subcellular architecture and dynamics at isotropic 100-nm resolution with 4Pi-SIM. Nature Methods 2024, 22: 335-347. PMID: 39715887, PMCID: PMC11810797, DOI: 10.1038/s41592-024-02515-z.Peer-Reviewed Original ResearchConceptsIsotropic optical resolutionThree-dimensional structured illumination microscopyStructured illumination microscopyWide-field microscopyDetected wavefrontSimultaneous imagingInterferometric microscopyDynamic behaviorOptical sectioningVolumetric imagesImage distortionOptical resolutionResolutionResolution results
2023
Membrane remodeling properties of the Parkinson’s disease protein LRRK2
Wang X, Espadas J, Wu Y, Cai S, Ge J, Shao L, Roux A, De Camilli P. Membrane remodeling properties of the Parkinson’s disease protein LRRK2. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2309698120. PMID: 37844218, PMCID: PMC10614619, DOI: 10.1073/pnas.2309698120.Peer-Reviewed Original ResearchCell cycle controls long-range calcium signaling in the regenerating epidermis
Moore J, Bhaskar D, Gao F, Matte-Martone C, Du S, Lathrop E, Ganesan S, Shao L, Norris R, Sanz N, Annusver K, Kasper M, Cox A, Hendry C, Rieck B, Krishnaswamy S, Greco V. Cell cycle controls long-range calcium signaling in the regenerating epidermis. Journal Of Cell Biology 2023, 222: e202302095. PMID: 37102999, PMCID: PMC10140546, DOI: 10.1083/jcb.202302095.Peer-Reviewed Original ResearchThree-dimensional structured illumination microscopy with enhanced axial resolution
Li X, Wu Y, Su Y, Rey-Suarez I, Matthaeus C, Updegrove T, Wei Z, Zhang L, Sasaki H, Li Y, Guo M, Giannini J, Vishwasrao H, Chen J, Lee S, Shao L, Liu H, Ramamurthi K, Taraska J, Upadhyaya A, La Riviere P, Shroff H. Three-dimensional structured illumination microscopy with enhanced axial resolution. Nature Biotechnology 2023, 41: 1307-1319. PMID: 36702897, PMCID: PMC10497409, DOI: 10.1038/s41587-022-01651-1.Peer-Reviewed Original Research
2022
Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9
Yang S, Park D, Manning L, Hill SE, Cao M, Xuan Z, Gonzalez I, Dong Y, Clark B, Shao L, Okeke I, Almoril-Porras A, Bai J, De Camilli P, Colón-Ramos DA. Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9. Neuron 2022, 110: 824-840.e10. PMID: 35065714, PMCID: PMC9017068, DOI: 10.1016/j.neuron.2021.12.031.Peer-Reviewed Original ResearchConceptsSynaptic vesicle cycleVesicle cyclePresynaptic autophagyAutophagosome biogenesisATG-9Only transmembrane proteinTrans-Golgi networkCellular degradation pathwayPresynaptic sitesActivity-dependent mannerTransmembrane proteinSynaptojanin 1Synaptic fociBiogenesisAutophagyNeuronal healthDegradation pathwayTraffickingPathwayParkinson's diseaseSynaptic activityNeuronal activityElegansSitesEndocytosis
2021
Differential adhesion regulates neurite placement via a retrograde zippering mechanism
Sengupta T, Koonce NL, Vázquez-Martínez N, Moyle MW, Duncan LH, Emerson SE, Han X, Shao L, Wu Y, Santella A, Fan L, Bao Z, Mohler W, Shroff H, Colón-Ramos DA. Differential adhesion regulates neurite placement via a retrograde zippering mechanism. ELife 2021, 10: e71171. PMID: 34783657, PMCID: PMC8843091, DOI: 10.7554/elife.71171.Peer-Reviewed Original ResearchConceptsDifferential adhesionDifferential adhesion mechanismsSYG-1SYG-2Developmental programEmbryonic developmentNeurite tipsZippering mechanismBiophysical principlesNeurite shaftSynaptic specificityBrain neuropilSingle neuriteLayers occursAlternate mechanismAdhesion mechanismExpressionNeuritesZipperingAdhesionMechanismOutgrowthStructural and developmental principles of neuropil assembly in C. elegans
Moyle MW, Barnes KM, Kuchroo M, Gonopolskiy A, Duncan LH, Sengupta T, Shao L, Guo M, Santella A, Christensen R, Kumar A, Wu Y, Moon KR, Wolf G, Krishnaswamy S, Bao Z, Shroff H, Mohler WA, Colón-Ramos DA. Structural and developmental principles of neuropil assembly in C. elegans. Nature 2021, 591: 99-104. PMID: 33627875, PMCID: PMC8385650, DOI: 10.1038/s41586-020-03169-5.Peer-Reviewed Original ResearchConceptsSpecific sensory organsNerve ringCaenorhabditis elegansC. elegansMuscle quadrantsNeuropil organizationDevelopmental principlesTissue organizationSensory organsBehavioral circuitsElegansPioneer neuronsCell positionDevelopmental sequenceStratified architectureTemporal progressionPrecise circuitsPacked neuronsUnique morphologyNeuronsSequenceOutgrowthAssemblyHierarchical developmentNeuropil
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