2021
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
Kroll F, Powell GT, Ghosh M, Gestri G, Antinucci P, Hearn TJ, Tunbak H, Lim S, Dennis HW, Fernandez JM, Whitmore D, Dreosti E, Wilson SW, Hoffman EJ, Rihel J. A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes. ELife 2021, 10: e59683. PMID: 33416493, PMCID: PMC7793621, DOI: 10.7554/elife.59683.Peer-Reviewed Original ResearchConceptsKnockout methodComplex mutant phenotypesLarge genetic screenRapid genetic screeningGenetic screenMutant phenotypeEffective CRISPRBiallelic knockoutMolecular rhythmsHuman genesInjected embryosCircadian clockLarval zebrafishBehavioral phenotypesMultiple genesComplex phenotypesCurrent CRISPRTriple knockoutAttractive modelGenesGenetic contributionZebrafishPhenotypeKnockoutCRISPR
2018
Zebrafish Models of Neurodevelopmental Disorders: Past, Present, and Future
Sakai C, Ijaz S, Hoffman EJ. Zebrafish Models of Neurodevelopmental Disorders: Past, Present, and Future. Frontiers In Molecular Neuroscience 2018, 11: 294. PMID: 30210288, PMCID: PMC6123572, DOI: 10.3389/fnmol.2018.00294.Peer-Reviewed Original ResearchGene functionZebrafish modelCRISPR/Cas9 geneSmall-molecule suppressorTransparent embryosZebrafish systemHigh-throughput pharmacological screensNeurodevelopmental disordersGenetic manipulationCas9 geneBehavioral phenotypesLarge progenyFunctional analysisLarval stagesPharmacological screensZebrafishRisk genesGenesModel systemExternal developmentPhenotypeCircuit-level mechanismsBasic mechanismsNeural circuitsNervous system
2010
Progress in Cytogenetics: Implications for Child Psychopathology
Hoffman EJ, State MW. Progress in Cytogenetics: Implications for Child Psychopathology. Journal Of The American Academy Of Child & Adolescent Psychiatry 2010, 49: 736-751. PMID: 20643309, DOI: 10.1016/j.jaac.2010.03.016.Peer-Reviewed Original ResearchConceptsChromosomal structureHuman genetic variationSequence of DNAChromosomal variationMicroarray-based detectionGenetic variationGenetic basisMolecular cytogeneticsGenetic underpinningsMicroarray technologySynapse functionKey discoveriesStructural variationsRecent findingsUnaffected individualsChromosomal studiesCytogenetic investigationsDevelopmental neuropsychiatric disordersCytogeneticsLight microscopyChromosomesGeneticsDNADramatic increasePhenotype