Kallol Gupta, PhD
Assistant Professor of Cell BiologyCards
Appointments
Additional Titles
Directors of Graduate Admission- BBS MCGD Track , Department of Cell Biology
Cell Biology DEI Committee member, Cell Biology
Yale West Campus DEI Committee member, Yale West Campus
Contact Info
Appointments
Additional Titles
Directors of Graduate Admission- BBS MCGD Track , Department of Cell Biology
Cell Biology DEI Committee member, Cell Biology
Yale West Campus DEI Committee member, Yale West Campus
Contact Info
Appointments
Additional Titles
Directors of Graduate Admission- BBS MCGD Track , Department of Cell Biology
Cell Biology DEI Committee member, Cell Biology
Yale West Campus DEI Committee member, Yale West Campus
Contact Info
About
Titles
Assistant Professor of Cell Biology
Directors of Graduate Admission- BBS MCGD Track , Department of Cell Biology; Cell Biology DEI Committee member, Cell Biology; Yale West Campus DEI Committee member, Yale West Campus
Biography
Trained as a chemist, I was introduced to the world of biology through the aquatic route, where my goal was to uncover the molecular diversity present in the venoms of marine cone snails and understand how it impairs the functions of key ion channels and transporters. This introduced and made me fascinated by the world of membrane proteins.
I subsequently moved to the University of Oxford as a Fellow of the 1851 Royal Commission. Here, my research led to a transformative advancement that enabled top-down mass spectrometric (MS) analysis of membrane protein-lipid complexes. Application of this approach yielded an atomistic understanding of how lipids act as key signaling molecules to regulate the structural and functional organization of membrane proteins.
In the Fall of 2018, I crossed the pond to start my own lab at Yale. Combining native mass spectrometry with an array of orthogonal experimental and computational approaches, our lab is focused on developing platforms that enable quantitative analysis of macromolecular protein complexes directly from the cellular environment. Applying this, the lab aims to understand how the spatiotemporal organization of membrane proteins and lipids regulates cellular physiology. For more, check out https://www.theguptalab.com/
Appointments
Cell Biology
Assistant ProfessorPrimary
Other Departments & Organizations
Education & Training
- Non Degree Program
- University of Oxford, Department of Chemistry (2018)
- Fellow of the 1851 Royal Commission
- Oxford University (2018)
- PhD
- Indian Institute of Science, Molecular Biophysics Unit (2013)
- BSc
- Presidency College, Chemistry (2005)
Research
Overview
Medical Subject Headings (MeSH)
ORCID
0000-0002-3557-3242- View Lab Website
Gupta Lab
Research at a Glance
Yale Co-Authors
Publications Timeline
Research Interests
Moitrayee Bhattacharyya, PhD
Aniruddha Panda
Jeff Coleman, PhD
Mandar Deepak Muzumdar, MD
Nikhil S. Malvankar
Sathish Ramakrishnan, PhD
Mass Spectrometry
Cell Membrane Permeability
Publications
2024
Author Correction: Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity
Gu Y, Guberman-Pfeffer M, Srikanth V, Shen C, Giska F, Gupta K, Londer Y, Samatey F, Batista V, Malvankar N. Author Correction: Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity. Nature Microbiology 2024, 1-1. PMID: 38684912, DOI: 10.1038/s41564-024-01702-0.Peer-Reviewed Original ResearchAltmetricConceptsA proteome-wide quantitative platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs.
Brown C, Ghosh S, McAllister R, Kumar M, Walker G, Sun E, Aman T, Panda A, Kumar S, Li W, Coleman J, Liu Y, Rothman JE, Bhattacharyya M, Gupta K. A proteome-wide quantitative platform for nanoscale spatially resolved extraction of membrane proteins into native nanodiscs. BioRxiv 2024 PMID: 38405833, DOI: 10.1101/2024.02.10.579775.Peer-Reviewed Original ResearchImaging nanoscale-spatial oligomeric organization of membrane proteins directly from native membranes at single-molecule resolution
Walker G, Brown C, Ge X, Kumar S, Muzumdar M, Gupta K, Bhattacharyya M. Imaging nanoscale-spatial oligomeric organization of membrane proteins directly from native membranes at single-molecule resolution. Biophysical Journal 2024, 123: 348a. DOI: 10.1016/j.bpj.2023.11.2115.Peer-Reviewed Original Research
2023
Oligomeric organization of membrane proteins from native membranes at nanoscale spatial and single-molecule resolution
Walker G, Brown C, Ge X, Kumar S, Muzumdar M, Gupta K, Bhattacharyya M. Oligomeric organization of membrane proteins from native membranes at nanoscale spatial and single-molecule resolution. Nature Nanotechnology 2023, 19: 85-94. PMID: 38012273, PMCID: PMC10981947, DOI: 10.1038/s41565-023-01547-4.Peer-Reviewed Original ResearchCitationsAltmetricConceptsMembrane proteinsNative membranesOligomeric organizationDiverse membrane proteinsMembrane protein biologyNative cell membranesTarget membrane proteinsNative membrane environmentSingle-molecule resolutionSingle-molecule platformReceptor tyrosine kinasesOligomeric distributionNative nanodiscsOligomerization statusProtein biologySmall GTPaseGrowth factor bindingMembrane environmentOligomeric assembliesTyrosine kinaseCritical regulatorOncogenic mutationsCell membraneProteinMembraneRoles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery
Sundaram R, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst A, Gupta K, Rothman J, Krishnakumar S. Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2309516120. PMID: 37590407, PMCID: PMC10450444, DOI: 10.1073/pnas.2309516120.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsCore protein machineryRelease-ready vesiclesSynaptic vesicle primingVesicle primingProtein machinerySingle-molecule imagingSNAREpin assemblyFunctional intermediatesFunctional reconstitutionMunc13DiacylglycerolCoordinated actionMunc18VesiclesMachineryComplete reconstitutionNew roleSelective effectDetailed characterizationChaperonesRate of caReconstitutionVAMP2ComplexinMutationsStudying Membrane Protein–Lipid Specificity through Direct Native Mass Spectrometric Analysis from Tunable Proteoliposomes
Panda A, Brown C, Gupta K. Studying Membrane Protein–Lipid Specificity through Direct Native Mass Spectrometric Analysis from Tunable Proteoliposomes. Journal Of The American Society For Mass Spectrometry 2023, 34: 1917-1927. PMID: 37432128, PMCID: PMC10932607, DOI: 10.1021/jasms.3c00110.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsIntegral membrane proteinsMembrane proteinsNative mass spectrometryTrafficking pathwaysPlasma membraneEukaryotic integral membrane proteinsEndoplasmic reticulumBiophysical propertiesMembrane protein assemblySynaptic vesiclesCellular trafficking pathwaysOrganellar membranesLipid specificityTransmembrane proteinProtein assembliesMembrane contextMass spectrometric analysisProteinNative mass spectrometric analysesVAMP2Lipid compositionExogenous ligandsLipid membranesIndividual lipidsMembraneATG9 vesicles comprise the seed membrane of mammalian autophagosomes
Olivas T, Wu Y, Yu S, Luan L, Choi P, Guinn E, Nag S, De Camilli P, Gupta K, Melia T. ATG9 vesicles comprise the seed membrane of mammalian autophagosomes. Journal Of Cell Biology 2023, 222: e202208088. PMID: 37115958, PMCID: PMC10148236, DOI: 10.1083/jcb.202208088.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsAtg9 vesiclesMammalian autophagosomesStyrene maleic acid lipid particlesLipid scramblase activityLC3-IIAutophagosomes formAutophagosome membraneMature autophagosomesScramblase activityAutophagosome formationAtg9Lipid transportMembrane growthAutophagosomesNanoscale organizationProtein-mediated transferProteinMembrane surface areaOrganellesVesiclesSeed membraneMembraneLipid particlesLipidsDifferent stagesDirect determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
Panda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsIntegral membrane proteinsMembrane proteinsOligomeric organizationOligomeric stateNative mass spectrometry analysisFunctional oligomeric stateKey membrane componentMass spectrometry analysisNMS analysisTarget membraneLipid bindingMembrane componentsProteolipid vesiclesMembrane compositionLipid compositionSpectrometry analysisLipid membranesNeurotransmitter releaseProteinMembraneLipidsMembrane propertiesDirect determinationBilayersTransportersStructure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity
Gu Y, Guberman-Pfeffer M, Srikanth V, Shen C, Giska F, Gupta K, Londer Y, Samatey F, Batista V, Malvankar N. Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity. Nature Microbiology 2023, 8: 284-298. PMID: 36732469, PMCID: PMC9999484, DOI: 10.1038/s41564-022-01315-5.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsNanowire assembliesHigh electron conductivityExtracellular electron transportNanowire networksExtracellular electron acceptorsG. sulfurreducensElectron conductivityCryogenic electron microscopy structureNanowiresHigh conductivityElectron microscopy structureSerine proteasesDifferent biochemical environmentsElectron transportMicroscopy structureDiverse speciesGeobacter speciesSulfurreducensConductivityImportant bacteriaCharge interactionsElectron acceptorBiochemical environmentSpeciesIdentifies mechanisms
2022
Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation
Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, Reinisch KM. Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation. Journal Of Cell Biology 2022, 221: e202207022. PMID: 36282247, PMCID: PMC9597353, DOI: 10.1083/jcb.202207022.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsEndoplasmic reticulumLipid dropletsProtein-mediated transferLipid transport proteinsLipid droplet formationLD biologyMitochondrial proteinsSecretory pathwayMass spectrometry analysisTerminal domainMitochondrial morphologyTransport proteinsLipid transportersCellular membranesLD metabolismMembrane contactX-ray structureSpectrometry analysisOrganellesGlycerophospholipidsProteinHydrophobic cavityFatty acidsLipidsMembrane
Academic Achievements and Community Involvement
activity American Society for Mass Spectrometry
Professional OrganizationsMemberDetails2019 - Presentactivity Biophysical Society
Professional OrganizationsMemberDetails2021 - Presenthonor Emerging Investigator
International AwardAmerican Society for Mass SpectrometryDetails06/01/2023United Statesactivity Direct detection of oligomeric membrane proteins from intact customizable lipid membranes
Oral PresentationSanibel Conference on Membrane Protein Mass SpectrometryDetails01/19/2023 - 01/23/2023St. Petersburg, FL, United StatesSponsored by American Society for Mass Spectrometryactivity Direct detection of oligomeric membrane proteins from intact customizable lipid membranes
Oral PresentationAnnual meeting of American Society for Mass SpectrometryDetails07/04/2022 - 07/08/2022Minneapolis, MN, United StatesSponsored by American Society for Mass Spectrometry
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West Campus Integrative Science & Technology Center
850 West Campus Drive
West Haven, CT 06516