2024
Heterogeneous Cardiac-Derived and Neural Crest–Derived Aortic Smooth Muscle Cells Exhibit Similar Transcriptional Changes After TGFβ Signaling Disruption
Ren P, Jiang B, Hassab A, Li G, Li W, Assi R, Tellides G. Heterogeneous Cardiac-Derived and Neural Crest–Derived Aortic Smooth Muscle Cells Exhibit Similar Transcriptional Changes After TGFβ Signaling Disruption. Arteriosclerosis Thrombosis And Vascular Biology 2024, 45: 260-276. PMID: 39697172, PMCID: PMC12053597, DOI: 10.1161/atvbaha.124.321706.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaAortic AneurysmCell LineageDisease Models, AnimalGene Expression ProfilingHomeobox Protein Nkx-2.5HumansMaleMarfan SyndromeMiceMice, Inbred C57BLMice, KnockoutMuscle, Smooth, VascularMyocytes, Smooth MuscleMyosin Heavy ChainsNeural CrestPhenotypeReceptor, Transforming Growth Factor-beta Type IIReceptors, Transforming Growth Factor betaSignal TransductionSingle-Cell AnalysisTranscription, GeneticTranscriptomeTransforming Growth Factor betaWnt1 ProteinConceptsSmooth muscle cell clustersSmooth muscle cellsAortic smooth muscle cellsNeural crest-derived smooth muscle cellsCardiac derivativesMurine aortic smooth muscle cellsNeural crest originReceptor deletionAortic rootAdult miceNeural crest progenitorsNKX2-5Proximal aortaTranscriptional changesMouse modelTGFB signalingMuscle cellsConditional deletionAdult human aortaEmbryological originIncreased expressionAnalyzed single-cell transcriptomesTGFB receptorsBasal stateAortic homeostasis
2019
Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State
Chen Z, Lu Y, Zhang K, Xiao Y, Lu J, Fan R. Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State. Advanced Science 2019, 6: 1801361. PMID: 31065513, PMCID: PMC6498135, DOI: 10.1002/advs.201801361.Peer-Reviewed Original ResearchImmune cellsLigand lipopolysaccharideTime pointsToll-like receptor 4 ligand lipopolysaccharideBasal stateIndividual immune cellsActivation stateHuman macrophage responseEffector responsesMultiple time pointsInflammatory programSingle-cell RNA sequencingMacrophage responsePathogenic challengeSecretion assaysCell populationsSame single cellTime courseHomogeneous cell populationSecretion analysisLongitudinal trackingRNA sequencingCellsHeterogeneous responseSequential measurements
2016
Platelet WDR1 suppresses platelet activity and is associated with cardiovascular disease
Montenont E, Echagarruga C, Allen N, Araldi E, Suarez Y, Berger JS. Platelet WDR1 suppresses platelet activity and is associated with cardiovascular disease. Blood 2016, 128: 2033-2042. PMID: 27609643, PMCID: PMC5073182, DOI: 10.1182/blood-2016-03-703157.Peer-Reviewed Original ResearchConceptsPlatelet activityCardiovascular diseaseMEG-01 cellsHyperreactive platelet phenotypeBasal intracellular calcium concentrationPathogenesis of atherothrombosisSex-matched controlsIntracellular calcium concentrationMessenger RNAMEG-01Healthy controlsClinical significancePlatelet-related genesPlatelet phenotypeBasal stateMegakaryoblastic cell line MEG-01Human megakaryoblastic cell line MEG-01Thrombin activationDiseaseCalcium concentrationKD phenotypeProtein levelsF-actin contentPlatelet messenger RNAPlatelet RNA
2015
Imaging human brown adipose tissue under room temperature conditions with 11C-MRB, a selective norepinephrine transporter PET ligand
Hwang JJ, Yeckel CW, Gallezot JD, Aguiar RB, Ersahin D, Gao H, Kapinos M, Nabulsi N, Huang Y, Cheng D, Carson RE, Sherwin R, Ding YS. Imaging human brown adipose tissue under room temperature conditions with 11C-MRB, a selective norepinephrine transporter PET ligand. Metabolism 2015, 64: 747-755. PMID: 25798999, PMCID: PMC4408242, DOI: 10.1016/j.metabol.2015.03.001.Peer-Reviewed Original ResearchConceptsBrown adipose tissueDistribution volume ratioSympathetic nervous systemAdipose tissueRole of BATCold stimulationFDG PET-CT imagingNorepinephrine transporterSupraclavicular brown adipose tissueHuman brown adipose tissueTotal body fatLean body massCold-stimulated conditionsBody temperaturePET-CT imagingBioelectrical impedance analysisCore body temperatureFDG uptakePET-CTOccipital cortexBody fatNervous systemBody compositionPET ligandBasal state
2011
Lymphocyte Surface Molecule Expression on Basal State and After Challenge: Association of Clinical Profile, Inflammation, Hematological and Biochemical Markers in Sickle Cell Anemia Patients
Cerqueira B, Vilas-Boas W, Zanette A, Clarencio J, Salvino M, Cezar R, Andrade D, Reis M, Goncalves M. Lymphocyte Surface Molecule Expression on Basal State and After Challenge: Association of Clinical Profile, Inflammation, Hematological and Biochemical Markers in Sickle Cell Anemia Patients. Blood 2011, 118: 2146. DOI: 10.1182/blood.v118.21.2146.2146.Peer-Reviewed Original ResearchSickle cell anemia patientsSoluble adhesion moleculesSickle cell anemiaSurface molecule expressionSCA patientsControl groupAnemia patientsAdhesion moleculesLymphocyte expressionLPS challengeMedical historyCD18 expressionMolecule expressionSoluble vascular adhesion moleculeBasal statePro-inflammatory interactionsEffector immune responsesVaso-occlusive episodesExpression of CD62LMarkers of inflammationChronic inflammatory stateTNF-alpha valuesVascular adhesion moleculesComplete medical historyExpression of CD11b
1998
In vivo phosphorylation of the epithelial sodium channel
Shimkets R, Lifton R, Canessa C. In vivo phosphorylation of the epithelial sodium channel. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 3301-3305. PMID: 9501257, PMCID: PMC19736, DOI: 10.1073/pnas.95.6.3301.Peer-Reviewed Original ResearchMeSH KeywordsAldosteroneAmilorideAmino Acid SequenceAnimalsColforsinCyclic AMP-Dependent Protein KinasesDogsEpithelial CellsEpithelial Sodium ChannelsInsulinMolecular Sequence DataNephronsPeptide MappingPhosphopeptidesPhosphorylationProtein Kinase CRatsSodium Channel AgonistsSodium ChannelsTransfectionConceptsCarboxyl terminusEpithelial sodium channelAlpha subunitGamma subunitsDe novo phosphorylationSubunit of ENaC.Stable cotransfectionVivo phosphorylationProtein kinaseEpithelial cell lineSodium channelsMolecular mechanismsActivity of ENaCPhosphorylationSubunitsCell linesTerminusProteinBetaKinaseCotransfectionBasal stateSerineThreonineENaC.
1995
Fluid absorption in isolated perfused colonic crypts.
Singh S, Binder H, Boron W, Geibel J. Fluid absorption in isolated perfused colonic crypts. Journal Of Clinical Investigation 1995, 96: 2373-2379. PMID: 7593625, PMCID: PMC185889, DOI: 10.1172/jci118294.Peer-Reviewed Original ResearchConceptsCrypt cellsNM vasoactive intestinal peptideFluid absorptionLarge intestinal functionColonic cryptsVasoactive intestinal peptideLamina propria cellsNet fluid secretionRat distal colonNet fluid absorptionDibutyryl cyclic AMPIntestinal peptideDistal colonIntestinal functionLamina propriaPathophysiological paradigmCrypt preparationMM acetylcholineBasal stateNeurohumoral agonistsFluid secretionCellular elementsEpithelial cellsIntestinal cryptsCrypts
1992
Na-K-Cl cotransport in the shark rectal gland. I. Regulation in the intact perfused gland
Forbush B, Haas M, Lytle C. Na-K-Cl cotransport in the shark rectal gland. I. Regulation in the intact perfused gland. American Journal Of Physiology 1992, 262: c1000-c1008. PMID: 1566806, DOI: 10.1152/ajpcell.1992.262.4.c1000.Peer-Reviewed Original ResearchConceptsNa-K-ClNa-K-Cl cotransport systemNa-K-Cl cotransporterVasoactive intestinal peptideCotransport systemVIP-stimulated increasesStimulation of secretionShark rectal glandK exitBasolateral membranePerfused glandShark Squalus acanthiasIntestinal peptideArterial-venous differencesDogfish shark Squalus acanthiasIntact glandsPerfusion solutionPerfusion periodBasal statePerfusionGlandInvestigate regulationRectal glandRinger's solutionTime course
1983
Splanchnic and Peripheral Disposal of Oral Glucose in Man
Katz L, Glickman M, Rapoport S, Ferrannini E, DeFronzo R. Splanchnic and Peripheral Disposal of Oral Glucose in Man. Diabetes 1983, 32: 675-679. PMID: 6862113, DOI: 10.2337/diab.32.7.675.Peer-Reviewed Original ResearchConceptsSplanchnic glucose outputOral glucoseGlucose ingestionVein catheterizationTotal splanchnic glucose outputGlucose uptakeNet splanchnic glucose outputLeg glucose uptakeFemoral vein catheterizationMin xOral glucose loadHepatic vein catheterizationBlood flow measurementsPeripheral disposalBasal periodGlucose loadMuscle uptakeGlucose disposalNormal menSplanchnic removalGlucose outputPeripheral tissuesPostabsorptive stateYoung volunteersBasal state
1975
Glucagon secretion in acute and chronic pancreatitis.
DONOWITZ M, HENDLER R, SPIRO H, BINDER H, FELIG P. Glucagon secretion in acute and chronic pancreatitis. Annals Of Internal Medicine 1975, 83: 778-81. PMID: 1200523, DOI: 10.7326/0003-4819-83-6-778.Peer-Reviewed Original ResearchConceptsAcute pancreatitisPancreatic insufficiencyAlpha-cell responsivenessChronic pancreatic insufficiencyBasal glucagon levelsPancreatic glucagon concentrationsInfusion of alaninePrevious pancreatitisGlucagon responseAcute episodeGlucagon levelsGlucagon secretionChronic pancreatitisPlasma glucagonGlucagon concentrationsInitial episodeHealthy controlsPancreatitisCell responsivenessPatientsBasal statePrevious attacksSensitive indexInitial attackInsufficiency
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