2023
MPL36, a major plasminogen (PLG) receptor in pathogenic Leptospira, has an essential role during infection
Zhu W, Passalia F, Hamond C, Abe C, Ko A, Barbosa A, Wunder E. MPL36, a major plasminogen (PLG) receptor in pathogenic Leptospira, has an essential role during infection. PLOS Pathogens 2023, 19: e1011313. PMID: 37486929, PMCID: PMC10399853, DOI: 10.1371/journal.ppat.1011313.Peer-Reviewed Original ResearchConceptsOuter membrane proteinsBacterial outer membrane proteinsC-terminal regionSurface-exposed regionsLeptospiral pathogenesisMDCK cell monolayersMembrane proteinsPathogenic LeptospiraImportant virulence factorMutant strainRecombinant proteinsPathogen's abilityVirulence mechanismsLysine residuesKoch's molecular postulatesBinding proteinPlasminogen receptorPathogen disseminationPathogenic spirochetesVirulence factorsProteinBacterial pathogensEssential roleMutantsHost tissues
2022
Heme-induced genes facilitate endosymbiont (Sodalis glossinidius) colonization of the tsetse fly (Glossina morsitans) midgut
Runyen-Janecky L, Scheutzow J, Farsin R, Cabo L, Wall K, Kuhn K, Amador R, D’Souza S, Vigneron A, Weiss B. Heme-induced genes facilitate endosymbiont (Sodalis glossinidius) colonization of the tsetse fly (Glossina morsitans) midgut. PLOS Neglected Tropical Diseases 2022, 16: e0010833. PMID: 36441823, PMCID: PMC9731421, DOI: 10.1371/journal.pntd.0010833.Peer-Reviewed Original ResearchConceptsTsetse midgutHeme environmentDNA-binding proteinsGene Ontology termsBlood mealTsetse fly midgutVertebrate bloodBacterial genesHost midgutOntology termsDisease-causing pathogensBiological functionsGene expressionMutant strainFucose transporterInsertion mutationsMetabolic processesHost competencyEnvironmental stressorsFly midgutMidgutGenesSodalisTsetse fliesHeme
2021
BB0259 Encompasses a Peptidoglycan Lytic Enzyme Function for Proper Assembly of Periplasmic Flagella in Borrelia burgdorferi
Xu H, Hu B, Flesher DA, Liu J, Motaleb MA. BB0259 Encompasses a Peptidoglycan Lytic Enzyme Function for Proper Assembly of Periplasmic Flagella in Borrelia burgdorferi. Frontiers In Microbiology 2021, 12: 692707. PMID: 34659138, PMCID: PMC8517470, DOI: 10.3389/fmicb.2021.692707.Peer-Reviewed Original ResearchPeriplasmic flagellaFlagellar rodProper assemblyFlagellar hookCryo-electron tomographyPG sacculusProtein homologsΕ-ProteobacteriaPeptidoglycan sacculusLytic transglycosylaseΓ-ProteobacteriaDistinct proteinsFlgJOuter membraneFunctional flagellaFilament assemblyFunctional domainsEnzyme functionCell wallMutant strainLyme disease spirocheteFlagellaEnzyme activityAssemblySacculus
2018
Lvr, a Signaling System That Controls Global Gene Regulation and Virulence in Pathogenic Leptospira
Adhikarla H, Wunder EA, Mechaly AE, Mehta S, Wang Z, Santos L, Bisht V, Diggle P, Murray G, Adler B, Lopez F, Townsend JP, Groisman E, Picardeau M, Buschiazzo A, Ko AI. Lvr, a Signaling System That Controls Global Gene Regulation and Virulence in Pathogenic Leptospira. Frontiers In Cellular And Infection Microbiology 2018, 8: 45. PMID: 29600195, PMCID: PMC5863495, DOI: 10.3389/fcimb.2018.00045.Peer-Reviewed Original ResearchConceptsResponse regulatorTwo-component system proteinsDNA-binding response regulatorGlobal transcriptional regulationHybrid histidine kinaseGlobal gene regulationNovel signal pathwayComprehensive genomic analysisPhosphotransfer assaysTCS genesHistidine kinaseGene duplicationTranscriptional regulationGene regulationCorresponding genesPhylogenetic analysisGenomic analysisMolecular basisMutant strainSystem proteinsSignaling systemInfection processGenesBranched pathwayVirulence
2014
Large-scale identification of chemically induced mutations in Drosophila melanogaster
Haelterman NA, Jiang L, Li Y, Bayat V, Sandoval H, Ugur B, Tan KL, Zhang K, Bei D, Xiong B, Charng WL, Busby T, Jawaid A, David G, Jaiswal M, Venken KJ, Yamamoto S, Chen R, Bellen HJ. Large-scale identification of chemically induced mutations in Drosophila melanogaster. Genome Research 2014, 24: 1707-1718. PMID: 25258387, PMCID: PMC4199363, DOI: 10.1101/gr.174615.114.Peer-Reviewed Original ResearchConceptsWhole-genome sequencingDrosophila X chromosomeThousands of polymorphismsEukaryotic model organismForward genetic screenChemical mutagenesis screenFunctions of thousandsPhenotype of interestLarge-scale identificationTime-consuming identificationHundreds of strainsSingle nucleotide variantsGenetic screenEssential genesDrosophila melanogasterModel organismsMutagenesis screenRescue lethalityX chromosomeMutant strainCandidate mutationsMolecular lesionsLarge duplicationChemical mutagensRough mapping
2010
mip1 containing mutations associated with mitochondrial disease causes mutagenesis and depletion of mtDNA in Saccharomyces cerevisiae
Stumpf J, Bailey C, Spell D, Stillwagon M, Anderson K, Copeland W. mip1 containing mutations associated with mitochondrial disease causes mutagenesis and depletion of mtDNA in Saccharomyces cerevisiae. Human Molecular Genetics 2010, 19: 2123-2133. PMID: 20185557, PMCID: PMC2865372, DOI: 10.1093/hmg/ddq089.Peer-Reviewed Original ResearchConceptsMitochondrial dysfunctionHuman pol gammaSaccharomyces cerevisiae orthologAssociated with mitochondrial diseasesDecreased polymerase activityAtaxia-neuropathy syndromeDepletion of mtDNADNA polymerase gammaDisease-associated mutationsMutations in vivoIncreased nucleotide poolOrthologous human mutationMtDNA replication defectsMtDNA mutagenesisMtDNA replicationProgressive external ophthalmoplegiaSaccharomyces cerevisiaeMutant strainMutant enzymesPol gammaHuman orthologPolymerase gammaConserved regionMtDNA depletionMtDNA
2009
Pseudomonas aeruginosa OspR is an oxidative stress sensing regulator that affects pigment production, antibiotic resistance and dissemination during infection
Lan L, Murray TS, Kazmierczak BI, He C. Pseudomonas aeruginosa OspR is an oxidative stress sensing regulator that affects pigment production, antibiotic resistance and dissemination during infection. Molecular Microbiology 2009, 75: 76-91. PMID: 19943895, PMCID: PMC2881571, DOI: 10.1111/j.1365-2958.2009.06955.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAnimalsAnti-Bacterial Agentsbeta-Lactam Resistancebeta-LactamsFemaleGene DeletionGene Expression Regulation, BacterialGlutathione PeroxidaseHydrogen PeroxideMiceMice, Inbred C57BLModels, BiologicalMutagenesis, Site-DirectedOxidative StressPigments, BiologicalPneumoniaPseudomonas aeruginosaPseudomonas InfectionsQuorum SensingRepressor ProteinsSignal TransductionStress, PhysiologicalTyrosineVirulenceConceptsOxidative stress sensingCys-24Stress sensingPigment productionNull mutant strainOxidative stressSerine substitution mutantsGlobal regulatorPromoter DNASubstitution mutantsAdditional genesInside hostsQuorum sensingCys residuesMutant strainConstitutive expressionMultiple pathwaysRegulatory effectsBeta-lactam resistanceGenesSignificant inductionRegulatorTyrosine metabolismOSPRP. aeruginosaPapA3 Is an Acyltransferase Required for Polyacyltrehalose Biosynthesis in Mycobacterium tuberculosis *
Hatzios SK, Schelle MW, Holsclaw CM, Behrens CR, Botyanszki Z, Lin FL, Carlson BL, Kumar P, Leary JA, Bertozzi CR. PapA3 Is an Acyltransferase Required for Polyacyltrehalose Biosynthesis in Mycobacterium tuberculosis *. Journal Of Biological Chemistry 2009, 284: 12745-12751. PMID: 19276083, PMCID: PMC2676004, DOI: 10.1074/jbc.m809088200.Peer-Reviewed Original ResearchConceptsUnusual cell wallBiosynthetic gene clusterSulfolipid-1Cell wall moleculesTrehalose-based glycolipidsGene clusterBiosynthetic machineryPutative acyltransferaseBacterial lipid extractsCell wallMutant strainBiosynthesisWall moleculesPalmitoyl groupsGenesGlycolipidsM. tuberculosisPathogenic M. tuberculosisAcyltransferaseAcyltransferase activityPAT productionTrehaloseLipid extractsComplementationMycobacterium tuberculosis
2008
Defining the role of the sugar transporter Sts1 in sexual development of the mushroom Schizophyllum commune
Kasper D, Hickernell T, Horton J. Defining the role of the sugar transporter Sts1 in sexual development of the mushroom Schizophyllum commune. The FASEB Journal 2008, 22: 822.7-822.7. DOI: 10.1096/fasebj.22.1_supplement.822.7.Peer-Reviewed Original ResearchB mating type locusMating-type locusType locusMushroom developmentPutative sugar transporter genesSugar transporter genesMating-type specificityNull mutant strainB mating lociSchizophyllum communeFungus Schizophyllum communeMushroom Schizophyllum communeLipopeptide pheromonesMating lociHaploid individualsFunctional copyTransporter geneMutant strainG proteinsType specificitySTS1LociSexual developmentType pathwayCompatible strains
2007
Enabling a Community to Dissect an Organism: Overview of the Neurospora Functional Genomics Project
Dunlap JC, Borkovich KA, Henn MR, Turner GE, Sachs MS, Glass NL, McCluskey K, Plamann M, Galagan JE, Birren BW, Weiss RL, Townsend JP, Loros JJ, Nelson MA, Lambreghts R, Colot HV, Park G, Collopy P, Ringelberg C, Crew C, Litvinkova L, DeCaprio D, Hood HM, Curilla S, Shi M, Crawford M, Koerhsen M, Montgomery P, Larson L, Pearson M, Kasuga T, Tian C, Baştürkmen M, Altamirano L, Xu J. Enabling a Community to Dissect an Organism: Overview of the Neurospora Functional Genomics Project. Advances In Genetics 2007, 57: 49-96. PMID: 17352902, PMCID: PMC3673015, DOI: 10.1016/s0065-2660(06)57002-6.Peer-Reviewed Original ResearchConceptsFunctional genomics projectsGenomics projectsFilamentous fungiFilamentous fungus NeurosporaFunctional genomic analysisNon-yeast fungiFunctional genomicsNeurospora genomeFungus NeurosporaNovel genesPositional cloningNeurospora crassaAntisense transcriptsGenomic analysisSNP mapAlternative promotersCDNA libraryExpression analysisGene replacementMutant strainSystematic disruptionExpression dataPhenotypic analysisNeurosporaConditions of growth
2005
Construction and Characterization of Genetically Modified Synechocystis sp. PCC 6803 Photosystem II Core Complexes Containing Carotenoids with Shorter π-Conjugation than β-Carotene*
Bautista J, Tracewell C, Schlodder E, Cunningham F, Brudvig G, Diner B. Construction and Characterization of Genetically Modified Synechocystis sp. PCC 6803 Photosystem II Core Complexes Containing Carotenoids with Shorter π-Conjugation than β-Carotene*. Journal Of Biological Chemistry 2005, 280: 38839-38850. PMID: 16159754, DOI: 10.1074/jbc.m504953200.Peer-Reviewed Original ResearchMeSH Keywordsbeta CaroteneCarotenoidsCationsChlorophyllChromatographyChromatography, High Pressure LiquidElectronsGene DeletionLightManganeseModels, ChemicalModels, MolecularMutationOxidation-ReductionOxidoreductasesOxygenPhotosynthetic Reaction Center Complex ProteinsPhotosystem II Protein ComplexPigmentationRhodobacter capsulatusSpectrophotometrySpectrophotometry, InfraredSynechocystisTemperatureTime FactorsTyrosineConceptsPhytoene desaturase geneII core complexesDesaturase genePS II core complexesSynechocystis spCore complexPS II assemblyCarotene desaturase genePhotosystem II core complexPCC 6803Rhodobacter capsulatusWild typeMutant strainRedox functionPhotosystem IISecondary electron transfer pathwayGenesElectron transfer pathwayLight-induced formationCarotenoidsSpChlorophyllConjugated pi-electron systemPathwayComplexes
2000
Visualizing protein dynamics in yeast with green fluorescent protein
Burd C. Visualizing protein dynamics in yeast with green fluorescent protein. Methods In Enzymology 2000, 327: 61-69. PMID: 11044974, DOI: 10.1016/s0076-6879(00)27267-4.Peer-Reviewed Original ResearchConceptsGreen fluorescent proteinProtein dynamicsUse of GFPFluorescent proteinYeast cell biologyProtein sortingRNA localizationIndividual cellular componentsChromosomal dynamicsProtein localizationKinase signalingCell biologyMutant strainMolecular tagsCellular componentsYeastProteinNucleocytoplasmicBroad arrayLocalizationSignalingBiologySortingMajor impactTags
1999
Differential Regulation of Salmonella typhimurium Type III Secreted Proteins by Pathogenicity Island 1 (SPI-1)-Encoded Transcriptional Activators InvF and HilA
Eichelberg K, Galán J. Differential Regulation of Salmonella typhimurium Type III Secreted Proteins by Pathogenicity Island 1 (SPI-1)-Encoded Transcriptional Activators InvF and HilA. Infection And Immunity 1999, 67: 4099-4105. PMID: 10417179, PMCID: PMC96710, DOI: 10.1128/iai.67.8.4099-4105.1999.Peer-Reviewed Original ResearchConceptsExpression of componentsSPI-1Secretion systemRegulatory proteinsType III protein secretion systemType III secretion machineryCellular responsesHost cellsFunction mutationsType III secretion systemInvasion-associated type III secretion systemProtein secretion systemTranscriptional regulatory proteinsComplex regulatory mechanismsPathogenicity island 1Secretion machineryCentisome 63Different genesRegulatory mechanismsMutant strainPathogenicity islandInvFIsland 1Differential regulationProtein
1998
Maize mitochondrial seryl-tRNA synthetase recognizes Escherichia coli tRNASer in vivo and in vitro
Rokov J, Söll D, Weygand-Durašević I. Maize mitochondrial seryl-tRNA synthetase recognizes Escherichia coli tRNASer in vivo and in vitro. Plant Molecular Biology 1998, 38: 497-502. PMID: 9747857, DOI: 10.1023/a:1006088516228.Peer-Reviewed Original ResearchConceptsSeryl-tRNA synthetaseMitochondrial seryl-tRNA synthetasePutative mature proteinSeryl-tRNA synthetasesEscherichia coliStructure/function relationshipsMature proteinGene sequencesMutant strainSignificant similarityFunctional identityN-terminalYeast tRNAMitochondrial functionFunction relationshipsProteinPoor substrateSynthetaseColiSynthetasesTRNAVivoCDNAMaizeEnzymeOsteocalcin production in primary osteoblast cultures derived from normal and Hyp mice.
Carpenter T, Moltz K, Ellis B, Andreoli M, McCarthy T, Centrella M, Bryan D, Gundberg C. Osteocalcin production in primary osteoblast cultures derived from normal and Hyp mice. Endocrinology 1998, 139: 35-43. PMID: 9421395, DOI: 10.1210/endo.139.1.5677.Peer-Reviewed Original ResearchConceptsPrimary osteoblast culturesOsteoblast culturesRegulation of osteocalcinMessenger RNAMurine osteoblastsOsteocalcin productionOsteocalcin messenger RNASpecies-specific effectsPrimary murine osteoblastsMaturation-dependent fashionHyp mouse modelHyp miceMutant strainOsteoblast differentiationMurine cellsCultured cellsHYP culturesMurine culturesOsteoblastsRNACell viabilityPrimary culturesRegulationAltered responseNormal litter matesOsteocalcin Production in Primary Osteoblast Cultures Derived from Normal and Hyp Mice
Carpenter T, Moltz K, Ellis B, Andreoli M, McCarthy T, Centrella M, Bryan D, Gundberg C. Osteocalcin Production in Primary Osteoblast Cultures Derived from Normal and Hyp Mice. Endocrinology 1998, 139: 35-43. DOI: 10.1210/en.139.1.35.Peer-Reviewed Original ResearchExposure to 1,25(OH)2D3Hyp micePrimary osteoblast culturesOsteoblast culturesOsteocalcin productionHuman X-linked hypophosphatemiaMurine osteoblastsMaturation-dependent fashionOsteocalcin messenger RNAHyp mouse modelEffects of 1,25(OH)2D3Response to 1,25(OH)2D3X-linked hypophosphatemiaMessenger RNASpecies-specific effectsInhibit osteoblast differentiationRegulation of osteocalcinMurine culturesIn vivo milieuMutant strainPrimary culturesStimulate osteocalcinDay 9MiceOsteocalcin
1995
Characterization of a Novel Schizosaccharomyces pombe Multidrug Resistance Transporter Conferring Brefeldin A Resistance
Turi T, Rose J. Characterization of a Novel Schizosaccharomyces pombe Multidrug Resistance Transporter Conferring Brefeldin A Resistance. Biochemical And Biophysical Research Communications 1995, 213: 410-418. PMID: 7646493, DOI: 10.1006/bbrc.1995.2147.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acid SequenceATP-Binding Cassette TransportersBase SequenceBinding SitesBrefeldin ACloning, MolecularCyclopentanesDNA, FungalDrug Resistance, MultipleFungal ProteinsMolecular Sequence DataOpen Reading FramesPancreatitis-Associated ProteinsRestriction MappingSchizosaccharomycesSchizosaccharomyces pombe ProteinsSequence AnalysisSequence HomologyConceptsTranscription factorsHbA2 expressionWild-type S. pombeS. pombe proteinPap1 transcription factorSignificant sequence similarityWild type SchizosaccharomycesMultiple transcription factorsPombe proteinsCRM1 genesS. pombeSequence similarityMammalian cellsTransport proteinsDifferent genesMutant strainBrefeldin AGolgi complexEndoplasmic reticulumProtein secretionGenesMultiple copiesSchizosaccharomycesCRM1A ResistanceRegulation of Salmonella Virulence by Two‐Component Regulatory Systems
Groisman E, Heffron F. Regulation of Salmonella Virulence by Two‐Component Regulatory Systems. 1995, 319-332. DOI: 10.1128/9781555818319.ch20.Peer-Reviewed Original ResearchTwo-component systemEnvZ/OmpR systemTwo-component regulatory systemClassical bacterial geneticsIdentification of lociPhoP/PhoQCrucial virulence factorTranscription regulatory factorsOuter membrane porins OmpCTissue culture model systemTranscriptional regulationBacterial geneticsVirulence defectBacterial pathogenesisMicrobial pathogenesisEnvironmental cuesCulture model systemSalmonella virulenceMutant strainRegulatory factorsPorins OmpCS. typhimuriumRegulatory systemVirulence determinantsAdaptive response
1994
The origin and evolution of species differences in Escherichia coli and Salmonella typhimurium
Ochman H, Groisman EA. The origin and evolution of species differences in Escherichia coli and Salmonella typhimurium. EXS 1994, 69: 479-493. PMID: 7994120, DOI: 10.1007/978-3-0348-7527-1_27.Peer-Reviewed Original ResearchConceptsSpecies-specific sequencesSalmonella chromosomeEscherichia coliCodon usage patternsOpen reading frameHost epithelial cellsCommon ancestorMap positionPhenotypic charactersReading frameBase compositionHorizontal transferSalmonella typhimuriumMutant strainGenetic differencesEnteric speciesBacterial speciesGenomePoint mutationsPhenotypic characteristicsSpeciesCorresponding regionChromosomesSpecies differencesEpithelial cells
1990
Yeast suppressor mutations and transfer RNA processing
Nichols M, Willis I, Söll D. Yeast suppressor mutations and transfer RNA processing. Methods In Enzymology 1990, 181: 377-394. PMID: 2199758, DOI: 10.1016/0076-6879(90)81137-j.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBlotting, NorthernChromosomes, FungalGenes, FungalIndicators and ReagentsMolecular Sequence DataMutationNucleic Acid ConformationNucleic Acid HybridizationRNA Polymerase IIIRNA Processing, Post-TranscriptionalRNA, TransferRNA, Transfer, SerSaccharomyces cerevisiaeSuppression, GeneticTranscription FactorsTranscription, GeneticConceptsTRNA genesMature-sized tRNAsRNA processing reactionsPrimer-directed mutagenesisAminoacyl-tRNA synthetaseTransfer RNA moleculesCognate aminoacyl-tRNA synthetaseRNA processingSuppressor mutationsTRNA locusElongation factorProtein biosynthesisRibosomal interactionsRNA moleculesMutant strainStructural proteinsPink coloniesTranscription efficiencyProcessing reactionsProtein synthesisSuppressor functionTRNALow template concentrationsGenesLoci
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply